Method for mass propagation of lycoris aurea through somatic embryogenesis
A large number of explant technology, applied in the field of artificial propagation and cultivation of plants, can solve the problems of low reproduction coefficient, low induction rate and redifferentiation rate, browning, etc., achieve stable genetic traits, reduce production costs, save Effect of nursery land occupation
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example 1
[0024] Induction and cultivation of example 1 callus
[0025] 1. Take the underground bulb of Hudixiao, peel off the outer scales, cut off the root, and cut off about 1 / 2 to 2 / 3 of the upper half of the bulb. Divide the bulbs with bulb discs into 4-8 pieces, shake and wash with 0.1% Amway washing liquid on the shaker for 15-20 minutes, rinse with running water for 60 minutes, and then rinse with 75% alcohol on the ultra-clean workbench for 30 seconds After cleaning 4 times with sterile water, sterilize with 1% sodium hypochlorite solution for 20-30 minutes (add a few drops of Tween 80 to the sodium hypochlorite solution), rinse with sterile water 4-5 times, and finally dry the small bulbs with sterile filter paper. surface moisture.
[0026] 2. Inoculate the sterilized Hudixiao bulb pieces directly into the adventitious bud induction medium: add in the MS basic medium, 3.0 mg / L of 6-benyl adenine (6-BA), indole acetic acid (IBA) 0.2mg / L, sucrose 30g / L, appropriate pH value; ...
example 2
[0029] The differentiation of example 2 callus and the rooting, transplanting of test-tube plantlet
[0030] 1. After culturing for 30-60 days, wait for the callus to grow to 2-3 cm, cut it and inoculate it again in the callus differentiation medium of the present invention, and carry out differentiation culture. The callus differentiation medium is MS medium as the basic medium, and the plant growth hormone 6-benzyl adenine (6-BA) 6.0mg / L, naphthaleneacetic acid (NAA) 0.2mg / L, sucrose 30g / L L, for proliferation culture. After more than 30 days of culture, the induction rate reaches 98%, and most of the callus can induce 5-6 clustered buds
[0031] 2. Choose robust test-tube plantlets, then inoculate them on 1 / 2 MS basic medium, indole butyric acid (IBA) 1.0mg / L, sucrose 60g / L, and carry out rooting induction. After 40 days of cultivation, the rooting rate reached 86%, and the average root number reached 8.5.
[0032]3. After rooting and cultivating the Hudixiao tissue cult...
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