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The rapid propagation method of Acanthopanax in vitro

A kind of thin-column five plus fast technology, applied in the field of artificial propagation and cultivation of plants, can solve the problems of natural reproduction difficulties, weakened plant vigor, out of stock of five plus bark, etc., to save land for seedlings, stabilize genetic traits, and shorten growth cycle effect

Inactive Publication Date: 2016-03-02
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acanthopanax acanthus is a famous and precious nourishing and strong medicine. It has the specific effects of dispelling rheumatism, nourishing the liver and kidney, and strengthening bones and muscles. At present, there are no large-scale artificial planting reports at home and abroad. The market supply is mainly based on the collection of wild resources. Due to the difficulty of natural reproduction Due to severe damage to the excavated root bark, the bark of Wujia has been out of stock in the domestic Chinese medicine market for decades. out of stock
However, long-term cutting propagation is an important reason for the weakening of plant vigor and virus infection, and seriously affects the resistance and quality of Acanthopanax sativa to adversity, and restricts the breeding process of Acanthopanax sativa

Method used

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  • The rapid propagation method of Acanthopanax in vitro
  • The rapid propagation method of Acanthopanax in vitro
  • The rapid propagation method of Acanthopanax in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Induction and cultivation of embodiment 1 axillary buds

[0031] 1. Take the young stems of Acanthopanax acanthus, shake and wash them with 0.1% Amway washing solution on a shaker for 15-20 minutes, rinse them with running water for 60 minutes, and then rinse them with 75% alcohol for 30 seconds on an ultra-clean workbench. After washing 4 times with bacterial water, wash with 0.1% HgCl 2 Sterilize for 50 to 60 minutes (add a few drops of Tween 80 to mercuric chloride), rinse with sterile water for 4 to 6 times, and finally dry the water on the surface of the seeds with sterile filter paper.

[0032] 2. Inoculate the sterilized stem section of Acanthopanax acanthus directly into the axillary bud induction medium: add 30 g / L sucrose (edible sugar) to the basic medium of WPM, appropriate pH value; culture temperature 25°C, light intensity 3000 Lux, Lighting time 16h, light culture.

[0033] 3. Cultivate 20 days after axillary bud germination aseptic seedling stem tip an...

Embodiment 2

[0034] Rooting, transplanting of induction of embodiment 2 clustering buds and test-tube plantlets

[0035] 1. After culturing for 30 days, wait for the sprouts to grow to 6-8 cm, re-inoculate the sprouts into the cluster bud induction medium of the present invention, and carry out proliferation culture. Cluster bud induction medium is to take WPM medium as basic medium, and add plant growth hormone ZT1mg / L and IBA0.5mg / L, sucrose (edible sugar) 30g / L, carry out proliferation culture. After culturing for more than 30 days, the induction rate reaches 98%, and most of the sprouts can induce clustered buds to reach 3-5.

[0036] 2. Select the test-tube seedlings with strong growth, soak them in the sterilized solution containing NAA0.2mg / L and paclobutrazol 10.0mg / L for 10min, then inoculate them on 1 / 2WPM basic medium, sucrose (edible sugar) ) 15g / L for rooting induction. After 40 days of cultivation, the rooting rate reached 86%, and the average root number reached 18.5.

[...

Embodiment 3

[0038] The induction of embodiment 3 axillary buds and the selection of culture medium

[0039]The sterilized stem segments were inoculated on 6 kinds of media, 1-2 plants were inoculated in each bottle, and the composition of the media was shown in Table 1. Subculture every 10 days and count the growth of explants. The culture conditions are temperature of 25°C, light intensity of 3000Lux, and light time of 16h.

[0040] Table 1 Induction of axillary buds and composition of culture medium

[0041]

[0042] 30 days after inoculation, statistics produced clustered bud number, adventitious bud incidence rate and adventitious bud growth status (Table 2), as can be seen in the WPM basal medium, add phytohormone IBA2.0mg / L, BA0.5mg / L and NAA0.2mg / L L, sucrose 30g / L, light culture is the best culture condition.

[0043] Table 2 Effects of Different Media on the Proliferation and Cultivation of Acanthopanax Acanthopanax

[0044]

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Abstract

The invention provides an in-vitro quick propagation method for acanthopanax gracilistylus. The large-scale production and propagation are executed to meet a market requirement. Acanthopanax gracilistylus tissue culture steps comprise: A, disinfecting an explant; B, obtaining germfree seedlings; C, growing and culturing axillary buds; D, inducing cluster buds; E, performing rooting culture; and F, performing seedling hardening and transplanting. According to a plant tissue culture method for propagating the acanthopanax gracilistylus, the influence caused by the external conditions is avoided, and the method can be performed in four seasons; the land occupied by seedlings is saved, and the production cost is lowered; furthermore, all good characters of a parent can be stored, and the inheritable character is stable. The method can be used for forming a large number of high-quality test tube seedlings within short time; the large-scale and industrial production is realized, and a large quantity of raw materials can be supplied to the industries such as ornamental horticulture and pharmacy.

Description

technical field [0001] The invention relates to a method for rapid propagation of sarcophagus, and belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique [0002] Acanthopanax was first recorded in the book of Materia Medica "Shen Nong's Materia Medica", and it is called "Acanthopanax bark", which is listed as top grade. "The smell is pungent and warm. It is mainly used for hernia and abdominal pain in trusted subordinates. It can benefit qi and treat diarrhea. Children can't do it, and gangrene is eroded." Since then, it has been included in "Famous Doctors" and "Donghua Zhenren Boiled Stone" and other medical works of all dynasties. Added records and descriptions. However, due to the lack of detailed descriptions of the original plants of Acanthopanax in ancient literature, and the relatively similar morphology of plants in the genus Aralis, it has been verified by previous research that the bark of Acanthopanax co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑生智韦敏汪琼吕晔
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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