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In-vitro rapid propagation method of double-petal Jasminum sambac

A jasmine and in vitro technology, applied in the field of artificial propagation and cultivation of plants, can solve the problems of weakening plant vigor, carrying viruses, restricting the breeding process of jasmine, etc., and achieves the effects of reducing production costs, stabilizing hereditary traits, and saving the land occupied by seedlings.

Inactive Publication Date: 2012-11-28
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term cutting propagation is an important reason for the weakening of plant vigor and virus, and seriously affects the resistance of jasmine to adversity and the quality and quality of flower fragrance, and restricts the breeding process of jasmine

Method used

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  • In-vitro rapid propagation method of double-petal Jasminum sambac
  • In-vitro rapid propagation method of double-petal Jasminum sambac
  • In-vitro rapid propagation method of double-petal Jasminum sambac

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Induction and cultivation of embodiment 1 axillary buds

[0030] 1. Take the young stems of double-lobe jasmine, shake and wash them with 0.1% Amway washing liquid on a shaker for 15-20 minutes, rinse them with running water for 60 minutes, and then rinse them with 75% alcohol for 30 seconds on an ultra-clean workbench. After washing 4 times with water, wash with 0.1% HgCl 2 Sterilize for 50-60 minutes (add a few drops of Tween 80 to mercuric chloride), rinse with sterile water for 4-5 times, and finally dry the water on the surface of the seeds with sterile filter paper.

[0031] 2. Inoculate the sterilized double-lobe jasmine stems directly into the axillary bud induction medium: add 30 g / L sucrose (edible sugar) to the basic medium of WPM, appropriate pH value; culture temperature 28 ± 1 ° C, light intensity 1000 ~3000lx, light time 10~16h, carry out light cultivation.

[0032] 3. Cultivate the stem tips of aseptic seedlings for about 20 days after the germination ...

Embodiment 2

[0033] Rooting, transplanting of induction of embodiment 2 clustering buds and test-tube plantlets

[0034] 1. After culturing for 30 days, wait for the sprouts to grow to 6-8 cm, re-inoculate the sprouts into the cluster bud induction medium of the present invention, and carry out proliferation culture. Cluster bud induction medium is to take WPM medium as basic medium, and add plant growth hormone ZT1mg / L and IBA0.5mg / L, sucrose (edible sugar) 30g / L, carry out proliferation culture. After culturing for more than 30 days, the induction rate reaches 98%, and most of the sprouts can induce clustered buds to reach 3-5.

[0035] 2. Select the test-tube seedlings that grow robustly, soak them in a sterilized solution containing NAA450mg / L for 10min, then inoculate them on 1 / 2MS basic medium, and use 15g / L sucrose (edible sugar) to induce rooting . After 40 days of cultivation, the rooting rate reached 86%, and the average root number reached 18.5.

[0036] 3. After rooting and ...

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Abstract

The invention provides a rapid propagation method of double-petal Jasminum sambac, which is used for large-scale production and propagation to meet the market needs. The tissue culture of double-petal Jasminum sambac comprises the following steps: A, disinfecting explants; B, obtaining sterile seedlings; C, growing and cultivating axillary buds; D, inducing cluster buds; E, rooting and cultivating; and F, exercising and transplanting seedlings. As the double-petal Jasminum sambac is propagated by the plant tissue culture method, the propagation is not influenced by external conditions and can be performed in all seasons while the occupied land of the seedlings is saved and the production cost is reduced; and moreover, all good characters of the parent can be maintained, and the hereditary character is stable. By using the method, a great number of good test tube seedlings can be formed in short time for large-scale and factory production, thereby providing abundant raw materials for the industries such as ornamental horticulture, spices, pharmacy and the like.

Description

technical field [0001] The invention relates to a rapid propagation method of double-lobe jasmine, which belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique [0002] Jasmine (Jasminum sambac L.) is a perennial evergreen shrub or slightly vine-like shrub in the family Oleaceae (Oleaceae). It is warm and humid in nature. It is native to India and Arabia. Cultivation and domestication have adapted to the climate in most parts of southern my country. As an important aromatic plant, jasmine is an important raw material for extracting essential oil and scenting jasmine tea. In China, the root or whole plant of jasmine has been cultivated and used as a kind of Mingjian herbal medicine because of its medical value such as treating diarrhea, relieving abdominal pain, and anti-inflammatory. Other researchers have found that the low concentration of jasmine tea aroma can effectively relieve the pain caused by autonomic nerve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 夏冰贺佳何丽斯汪仁李晓丹江玉梅王忠
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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