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Novel and high-efficiency tissue culture and rapid propagation method of cymbidium

A technology of tissue culture and Cymbidium, which is applied in the field of artificial propagation and cultivation of plants, can solve the problems of low reproduction coefficient, achieve the effects of stable genetic traits, save land for seedling cultivation, and reduce production costs

Inactive Publication Date: 2013-09-11
NANJING YIMEI BIOTECHOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the tissue culture of Cymbidium is based on the new shoots with growth points as explants to induce pseudoprotocorms, thereby inducing plants to achieve regeneration, and the reproduction coefficient is low

Method used

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  • Novel and high-efficiency tissue culture and rapid propagation method of cymbidium
  • Novel and high-efficiency tissue culture and rapid propagation method of cymbidium
  • Novel and high-efficiency tissue culture and rapid propagation method of cymbidium

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Experimental program
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Effect test

Embodiment 1

[0029] Induction and cultivation of embodiment 1 adventitious buds

[0030] 1. Take the new leaves of Cymbidium, shake and wash them with 0.1% Amway washing solution on a shaker for 5-10 minutes, rinse them with running water for 10-20 minutes, and then rinse them with 75% alcohol for 30 seconds on an ultra-clean workbench. After washing 4 times with bacterial water, wash with 0.1% HgCl 2 Disinfect for 5 to 15 minutes (add a few drops of Tween 80 to mercuric chloride), rinse with sterile water for 4 to 5 times, and finally dry the surface with sterile filter paper.

[0031] 2. Cut the sterilized Cymbidium leaves into 1.0-1.5cm fragments and inoculate them into the following medium: add NAA0.1mg / L+BA0.4mg / L+2,4- D2.0mg / L, sucrose (edible sugar) 30g / L, appropriate pH value; culture temperature 28±1°C, light intensity 1000-3000lx, light time 10-16h, light culture, induce callus production.

[0032] 3. Inoculate the callus with good morphology and high vitality obtained after in...

Embodiment 2

[0033] Rooting, transplanting of induction of embodiment 2 clustering buds and test-tube plantlets

[0034] 1. After cultivating for 30 days, when the sprouts grow to 6-8cm, the sprouts are reinoculated into the subculture propagation medium of the present invention to carry out proliferation cultivation. Cluster bud induction medium is based on WPM medium, and added plant growth hormone NAA0.1mg / L+BA0.4mg / L, sucrose (edible sugar) 30g / L, for proliferation culture. After culturing for more than 30 days, the induction rate reaches 98%, and most of the sprouts can induce clustered buds to reach 3-5 plants.

[0035] 2. Choose robust test-tube plantlets, inoculate them on 1 / 2 MS basic medium, NAA0.5mg / L, sucrose (edible sugar) 15g / L, and carry out rooting induction. After 40 days of cultivation, the rooting rate reached 92%, and the average root number reached 3.5.

[0036] 3. After rooting and culturing the Cymbidium cymbidium seedlings for 1-1.5 months, start hardening and tra...

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Abstract

The invention provides a novel and high-efficiency tissue culture and rapid propagation method of cymbidium, which is used for realizing large-scale production and propagation to meet market demands. The tissue culture method of the cymbidium comprises the following steps of: A, sterilizing explants; B, inducing callus; C, inducing adventitious buds; D, inducing cluster buds; E, carrying out rooting culture; and F, exercising seedlings and transplanting. The cymbidium can be propagated by the plant tissue culture method, the method can not be influenced by external conditions and can be carried out all the year round, the floor space of nursery stocks can be saved, the production cost can be reduced, all the merits of a parent body can be maintained, and the inheritable character is stable. After the method is used, a mass of good test-tube plantlets can be formed within a short period of time, the large-scale and industrial production can be carried out, and a mass of seedlings can be provided for industries of ornamental horticulture and the like.

Description

technical field [0001] The invention relates to a rapid propagation method of cymbidium and belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique [0002] Cymbidium grandiflorium (Cymbidium grandiflorium), also known as Himbiran, is a perennial herbaceous plant belonging to the Orchidaceae family. Its source is the large-flowered epiphytic species, small-flowered vertical species and some ground orchids that have been artificially crossed for more than 100 years. The leaves are long and green, the flowers are rough and magnificent, and it is a world-renowned "orchid star". It has the elegant fragrance of Chinese orchid and the richness and variety of western orchid. It is very popular in the international flower market and is deeply loved by flower lovers. However, there are more and more manufacturers of Cymbidium, and the market competition is fierce. This requires that new varieties are constantly produced to g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 汪仁贺佳王广文王进全
Owner NANJING YIMEI BIOTECHOLOGY
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