A method for mass propagation of Hudixiao by somatic embryogenesis

Abstract

The invention provides a method for mass propagation of lycoris aurea through somatic embryogenesis. The lycoris aurea tissue culture method for scale production and propagation so as to meet the market demand comprises the steps of A, explant disinfection, B, adventitious bud induction, C, callus induction, D, callus propagation, E callus redifferentiation, F, rooting culture, and H, seedling exercising and transplanting. According to the method, herbicide picloram is applied to tissue culture of lycoris aurea for the first time, stable lycoris aurea tissues capable of being propagated on a large scale are obtained, the callus induction rate reaches 95% or above, and the propagation coefficient of the lycoris aurea is at least increased by 8-10 times. The production cost is reduced, furthermore, the whole fine properties of a parent body can be preserved, and the inheritable character is stable. Through the method, a large number of excellent test-tube seedlings can be formed within a short period of time, large-scale industrialized production is conducted, and a lot of raw materials are provided for ornamental horticulture, pharmacy and other industries as well as the follow-up study of the lycoris aurea.

Description

technical field

[0001] The invention relates to a method for mass propagation of somatic embryogenesis, and belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique

[0002] Hudixiao (Lycoris aurea Herb.), also known as Lycoris aurea Herb., is mainly produced in my country and distributed in 16 provinces in southwest, east, south, and northwest China, and a small amount is also distributed in Japan and Myanmar. The flowers and leaves of Hudixiao have high ornamental value. Its flowers are golden yellow, tall and straight, and the flower shape is peculiar; after flowering, the leaves can be seen in winter and spring. In some countries such as Japan and the Netherlands, it is widely cultivated in roads and gardens, and it is a popular garden ornamental plant.

[0003] In addition, Hudixiao also has high medicinal value, and its bulb is a good raw material for extracting galantamine. The content of galantamine in Hudixiao...

Examples

example 1

[0024] Induction and cultivation of example 1 callus

[0025] 1. Take the underground bulb of Hudixiao, peel off the outer scales, cut off the root, and cut off about 1 / 2 to 2 / 3 of the upper half of the bulb. Divide the bulbs with bulb discs into 4-8 pieces, shake and wash with 0.1% Amway washing liquid on the shaker for 15-20 minutes, rinse with running water for 60 minutes, and then rinse with 75% alcohol on the ultra-clean workbench for 30 seconds After cleaning 4 times with sterile water, sterilize with 1% sodium hypochlorite solution for 20-30 minutes (add a few drops of Tween 80 to the sodium hypochlorite solution), rinse with sterile water 4-5 times, and finally dry the small bulbs with sterile filter paper. surface moisture.

[0026] 2. Inoculate the sterilized Hudixiao bulb pieces directly into the adventitious bud induction medium: add in the MS basic medium, 3.0 mg / L of 6-benyl adenine (6-BA), indole acetic acid (IBA) 0.2mg / L, sucrose 30g / L, appropriate pH value; ...

example 2

[0029] The differentiation of example 2 callus and the rooting, transplanting of test-tube plantlet

[0030] 1. After culturing for 30-60 days, wait for the callus to grow to 2-3 cm, cut it and inoculate it again in the callus differentiation medium of the present invention, and carry out differentiation culture. The callus differentiation medium is MS medium as the basic medium, and the plant growth hormone 6-benzyl adenine (6-BA) 6.0mg / L, naphthaleneacetic acid (NAA) 0.2mg / L, sucrose 30g / L L, for proliferation culture. After more than 30 days of culture, the induction rate reaches 98%, and most of the callus can induce 5-6 clustered buds

[0031] 2. Choose robust test-tube plantlets, then inoculate them on 1 / 2 MS basic medium, indole butyric acid (IBA) 1.0mg / L, sucrose 60g / L, and carry out rooting induction. After 40 days of cultivation, the rooting rate reached 86%, and the average root number reached 8.5.

[0032]3. After rooting and cultivating the Hudixiao tissue cult...

technical field

[0001] The invention relates to a method for mass propagation of somatic embryogenesis, and belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique

[0002] Hudixiao (Lycoris aurea Herb.), also known as Lycoris aurea Herb., is mainly produced in my country and distributed in 16 provinces in southwest, east, south, and northwest China, and a small amount is also distributed in Japan and Myanmar. The flowers and leaves of Hudixiao have high ornamental value. Its flowers are golden yellow, tall and straight, and the flower shape is peculiar; after flowering, the leaves can be seen in winter and spring. In some countries such as Japan and the Netherlands, it is widely cultivated in roads and gardens, and it is a popular garden ornamental plant.

[0003] In addition, Hudixiao also has high medicinal value, and its bulb is a good raw material for extracting galantamine. The content of galantamine in Hudixiao...

Examples

example 1

[0024] Induction and cultivation of example 1 callus

[0025] 1. Take the underground bulb of Hudixiao, peel off the outer scales, cut off the root, and cut off about 1 / 2 to 2 / 3 of the upper half of the bulb. Divide the bulbs with bulb discs into 4-8 pieces, shake and wash with 0.1% Amway washing liquid on the shaker for 15-20 minutes, rinse with running water for 60 minutes, and then rinse with 75% alcohol on the ultra-clean workbench for 30 seconds After cleaning 4 times with sterile water, sterilize with 1% sodium hypochlorite solution for 20-30 minutes (add a few drops of Tween 80 to the sodium hypochlorite solution), rinse with sterile water 4-5 times, and finally dry the small bulbs with sterile filter paper. surface moisture.

[0026] 2. Inoculate the sterilized Hudixiao bulb pieces directly into the adventitious bud induction medium: add in the MS basic medium, 3.0 mg / L of 6-benyl adenine (6-BA), indole acetic acid (IBA) 0.2mg / L, sucrose 30g / L, appropriate pH value; ...

example 2

[0029] The differentiation of example 2 callus and the rooting, transplanting of test-tube plantlet

[0030] 1. After culturing for 30-60 days, wait for the callus to grow to 2-3 cm, cut it and inoculate it again in the callus differentiation medium of the present invention, and carry out differentiation culture. The callus differentiation medium is MS medium as the basic medium, and the plant growth hormone 6-benzyl adenine (6-BA) 6.0mg / L, naphthaleneacetic acid (NAA) 0.2mg / L, sucrose 30g / L L, for proliferation culture. After more than 30 days of culture, the induction rate reaches 98%, and most of the callus can induce 5-6 clustered buds

[0031] 2. Choose robust test-tube plantlets, then inoculate them on 1 / 2 MS basic medium, indole butyric acid (IBA) 1.0mg / L, sucrose 60g / L, and carry out rooting induction. After 40 days of cultivation, the rooting rate reached 86%, and the average root number reached 8.5.

[0032]3. After rooting and cultivating the Hudixiao tissue cult...

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