36 results about "Lycoris aurea" patented technology

The invention discloses a GPA planting method of Lycoris aurea. Harvested flowers of the Lycoris aurea can be used as raw medicinal materials for extracting galanthamine. The method includes the six main steps of plantation building, propagation method implementation, planting, field management, pest control, and harvesting, processing and storage. By replacing wild resources with artificially-cultivated Lycoris aurea resources, the ecological environment can be effectively projected, water and soil loss can be prevented, and market supply of raw medicinal materials of the galanthamine and clinical medication safety of the galanthamine can be guaranteed as well. Because the overground flowers of the Lycoris aurea are used as the raw medicinal materials for extracting the galanthamine, labor intensity is small, the proportion of labor cost is small, and production cost is low. The standard operating procedure of each production link is worked out for the first time. The content of the galanthamine in the dried flowers, used as the raw medicinal materials, of the Lycoris aurea produced through the method is much higher than that in bulbs used as raw medicinal materials for producing galanthamine in a pharmaceutical factory in the prior art. Lycoris aurea industrialization and GAP standardized artificial cultivation are achieved through the method.

The invention discloses a method for extracting galanthamine by using lycoris aurea. The inflorescence of the lycoris aurea is taken as a raw material for extracting a galanthamine drug. An optimal method for recovering, drying and storing the inflorescence crude drug of the lycoris aurea and an optimal extraction technology are explored. The quality standard of the inflorescence crude drug of the lycoris aurea is worked out. By adopting the technical scheme, the extinct wild resource and ecological environment can be effectively protected, the clinical medication safety can be ensured by continuous long-term benefits; the method is low in labor intensity, small in proportion of labor costs, and low in production cost; the condition that the content of galanthamine in the produced dry flower of the crude drug lycoris aurea is much higher than that of the requirements of the crude drug bulb for producing the galanthamine in a pharmaceutical factory in the past is ensured, the extracted economic benefits, social benefits and ecological benefits are much superior to those of the traditional method, achievement of industrial production is facilitated, and the problem of supply of the galanthamine crude drug is thoroughly solved.

The invention relates to the technical field of veterinary medicine, in particular to a composition for controlling hog cholera. The composition comprises the following raw materials: winter cherry, Eucalyptus robusta leaves, Polygonum perfoliatum, atriplex centralasiatica, lycoris aurea, rhizoma anemarrhenae, pyrrosia lingua, inula herb, celandine, ardisiae japonica, cortex lycii radicis, blackberry lily, radices trichosanthis, Biebersteinia heterostemon, pegaeophyton scapiflorum, herba cepbalanoplosis segeti, corydalis boweri hemsl, elsholtzia, rehmanniachingiili, Oldenlandia diffusa and magnolia flower. The composition in the invention has an obvious control effect for hog cholera, so that the morbidity of hog cholera is controlled within 2.05%, the cure rate for hog cholera is more than 94%, the pathogenic bacteria and viruses thereof infected with hog cholera are effectively killed, and the composition has wide spectrum; moreover, the immune resistance for pigs is also improved, the stress resistance is improved, the risk of being infected with diseases is reduced, the control effect for hog cholera is effectively improved, and the composition has vitally important significance for improving pig production performance, reducing financial loss and promoting continuous and healthy development for pig breeding industry.

The invention discloses a method for rapid propagation of lycoris plant seedlings through hydroponics and aims at providing a lycoris plant asexual rapid propagation method with high reproductive rate for industrialization production of the lycoris plant seedlings. The technical scheme comprises mechanical cutting and induction of adventitious shoots through water pipelines. The method includes following steps: (1) cutting seedballs on a special lycoris seedball cutting machine according to a shape of a Chinese character mi; (2) soaking mother balls in culture solution for 30 minutes, fishing out and drying in shade; (3) inducing adventitious shoots through the water pipelines; and (4) transplanting in a greenhouse. The method for rapid propagation of lycoris plant seedlings through the hydroponics can greatly improve reproductive rate of lycoris aurea, reproduction ratio of one mother ball is 14 times of that of a natural son ball, and the method is suitable for industrialization large-scale production of the lycoris plant seedlings.

The invention discloses a plant facial cleansing fluid and a using method thereof. The facial cleansing fluid is prepared from mixed fresh juice comprising fresh hibiscus flower, fresh day lily flower, fresh mint leaf and auxiliary traditional Chinese medicine fresh lycoris aurea flower in a mass ratio of (2.5-3.5):(0.5-1.5):(0.2-0.8):(0.01-0.03), and optimally 3.0:1.0:0.5:0.02. The mixed plant juice is sealed in a small plastic bag, frozen and stored in a shadow place, and the preservation period is 6 months. Before the facial cleansing fluid is used for cleaning face, the facial cleansing fluid is unfrozen at normal temperature or in warm water at about 40 DEG C, and should be used up in 2 hours after unfreezing. The plant facial cleansing fluid is extracted from natural materials, and is good in facial cleansing effect; after the facial cleansing fluid is used for a period, the face is tender in hand feel, acne and the like can be improved, and face looks rosy. The facial cleansing fluid can be developed into a novel high-quality plant facial cleansing fluid commodity.

The invention relates to a cytochrome P450 reductase and a coding gene and application thereof. The invention discloses a cytochrome P450 reductase 2 of an amaryllidaceae plant lycoris aurea for the first time, and the cytochrome P450 reductase 2 has good coenzyme specificity and can assist a cytochrome P450 enzyme to develop the catalytic activity to oxidatively modify a substrate synthetic product thereof. The invention further discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier for expressing the cytochrome P450 reductase and a method for producing a caffeic acid.

The invention relates to cytochrome P450 reductase as well as coding gene and application thereof. The invention discloses cytochrome P450 reductase 1 of lycoris aurea as an amaryllidaceae plant for the first time; the cytochrome P450 reductase 1 has favorable coenzyme specificity and can be used for assisting a cytochrome P450 enzyme in exerting catalytic activity to modify a synthetic product of a substrate of the cytochrome P450 enzyme in an oxidative manner. The invention also discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier and a host cell for expressing the cytochrome P450 reductase and a method for producing p-coumaric acid.

The invention discloses a plant health care foot-soaking liquid and an application method thereof. The health care foot-soaking liquid is formed by the mixed fresh juice of fresh hibiscus bark, fresh day lily roots, rice oil bran and auxiliary Chinese herb lycoris aurea bulbs, wherein the mass ratio of the fresh hibiscus bark to the fresh day lily roots, the rice oil bran and the lycoris aurea bulbs in the mixed plant juice is (0.8-1.2): (1.2-1.8): (0.3-0.7): (0.02-0.04), preferably 1.0: 1.5: 0.5: 0.03. The mixed plant juice is packaged and sealed in small plastic bags, and kept in dark place after being frozen, and the quality guarantee period of the mixed plant juice is 6 months. Before soaking foot, the foot-soaking liquid is unfrozen at the normal temperature or in warm water at about 40 DEG C; after being unfrozen, the foot-soaking liquid must be used completely in 2 hours, and if in combination with foot bottom massage, the plant health care foot-soaking liquid is better in effect. The plant health care foot-soaking liquid can be developed into a novel good-quality plant health care foot-soaking liquid commodity.

The invention discloses a method for rapid propagation of lycoris plant seedlings through hydroponics and aims at providing a lycoris plant asexual rapid propagation method with high reproductive rate for industrialization production of the lycoris plant seedlings. The technical scheme comprises mechanical cutting and induction of adventitious shoots through water pipelines. The method includes following steps: (1) cutting seedballs on a special lycoris seedball cutting machine according to a shape of a Chinese character mi; (2) soaking mother balls in culture solution for 30 minutes, fishing out and drying in shade; (3) inducing adventitious shoots through the water pipelines; and (4) transplanting in a greenhouse. The method for rapid propagation of lycoris plant seedlings through the hydroponics can greatly improve reproductive rate of lycoris aurea, reproduction ratio of one mother ball is 14 times of that of a natural son ball, and the method is suitable for industrialization large-scale production of the lycoris plant seedlings.

The invention relates to a method for extracting galanthamine from lycoris aurea. The invention aims at carrying out improvement against the problems of low yield, high cost and weak market competitiveness of the existing method and establishing the method for extracting the galanthamine from the lycoris aurea, which has the industrial operatability. The method comprises the following steps: carrying out pretreatment on raw materials; carrying out the pretreatment by a cation exchange resin column; preparing the galanthamine by the thin-layer chromatography method (TCL method); extracting: smashing the lycoris aurea, then using methanol for heat reflux extraction, and concentrating extraction liquid till obtaining a liquid extract; separating: using 2% of acetic acid for dissolving the liquid extract and using resin for absorption; converting into galanthamine hydrobromide: introducing hydrogen bromide gas for obtaining a crude product II of galanthamine hydrobromide; and purifying the galanthamine hydrobromide. The method has the benefits that due to the adoption of the new extraction process, the method can simplify the operation, avoid the extraction process step, reduce the loss, improve the yield, reduce the cost and enhance the market competitiveness of the product.

The invention discloses a plant facial cleansing fluid and a using method thereof. The facial cleansing fluid is prepared from mixed fresh juice comprising fresh hibiscus flower, fresh day lily flower, fresh mint leaf and auxiliary traditional Chinese medicine fresh lycoris aurea flower in a mass ratio of (2.5-3.5):(0.5-1.5):(0.2-0.8):(0.01-0.03), and optimally 3.0:1.0:0.5:0.02. The mixed plant juice is sealed in a small plastic bag, frozen and stored in a shadow place, and the preservation period is 6 months. Before the facial cleansing fluid is used for cleaning face, the facial cleansing fluid is unfrozen at normal temperature or in warm water at about 40 DEG C, and should be used up in 2 hours after unfreezing. The plant facial cleansing fluid is extracted from natural materials, and is good in facial cleansing effect; after the facial cleansing fluid is used for a period, the face is tender in hand feel, acne and the like can be improved, and face looks rosy. The facial cleansing fluid can be developed into a novel high-quality plant facial cleansing fluid commodity.

The invention provides a method for mass propagation of lycoris aurea through somatic embryogenesis. The lycoris aurea tissue culture method for scale production and propagation so as to meet the market demand comprises the steps of A, explant disinfection, B, adventitious bud induction, C, callus induction, D, callus propagation, E callus redifferentiation, F, rooting culture, and H, seedling exercising and transplanting. According to the method, herbicide picloram is applied to tissue culture of lycoris aurea for the first time, stable lycoris aurea tissues capable of being propagated on a large scale are obtained, the callus induction rate reaches 95% or above, and the propagation coefficient of the lycoris aurea is at least increased by 8-10 times. The production cost is reduced, furthermore, the whole fine properties of a parent body can be preserved, and the inheritable character is stable. Through the method, a large number of excellent test-tube seedlings can be formed within a short period of time, large-scale industrialized production is conducted, and a lot of raw materials are provided for ornamental horticulture, pharmacy and other industries as well as the follow-up study of the lycoris aurea.

The invention relates to an ABC transport protein and a coding gene and application thereof. The invention discloses the ABC transport protein of lycoris aurea as an amaryllidaceae plant for the first time, and the ABC transport protein has the functions of being combined with lycorine and enabling the lycorine to be transported across cell membranes. The invention also discloses polynucleotide for coding the ABC transporter protein, a vector and a host cell for expressing the ABC transporter protein, and a method for realizing inward transport of lycorine across a biological membrane.