Method for separating and screening out antagonistic bacterium from lycoris aurea bulb

Abstract

The invention relates to a method for separating and screening out an antagonistic endophytic bacterium from a lycoris aurea bulb. According to the method, by using an endophyte separation method, an endophytic bacterium is obtained by separating the endophytic bacterium from the lycoris aurea bulb; an improved confrontation culture method is adopted, test magnaporthe grisea is dripped in the center of a flat plate, and is cultured for 2-3 days; four crosswise corners around the magnaporthe grisea are inoculated with four bacterial strains respectively, and culture is carried out for 5-6 days; through observation, a bacterium having a bacteriostatic effect is the antagonistic bacterium obtained through screening. The antagonistic bacterium having an obvious inhibiting effect on magnaporthe grisea of rice is obtained; an antimicrobial spectrum experiment proves that the antagonistic bacterium also has an inhibiting effect on fusarium graminearum, aspergillus fumigatus and candida albicans. By adopting the method disclosed by the invention, the screening efficiency can be remarkably improved, and the antagonistic bacterium obtained by separation can be used as a biocontrol bacterium for being prepared into a microbial preparation, thus providing a basis for biological control over diseases.

Description

technical field [0001] The invention belongs to the field of biological control bacteria, and in particular relates to a method for isolating and screening antagonistic bacteria from Hudixiao bulbs. Background technique [0002] Bacteria living in the intercellular space or in cells of healthy plant tissues and organs are called endophytic bacteria, some of which are antagonistic microorganisms of pathogenic bacteria. As a member of the plant micro-ecosystem, some endophytic bacteria can inhibit pathogenic bacteria by producing antibacterial active substances such as antibiotics and hydrolytic enzymes. The use of microorganisms to control plant fungal diseases has the advantages of no pollution to the environment, no harm to humans and animals, and slowing down the drug resistance of pathogenic bacteria. Researchers have isolated antagonistic bacteria from rice, cotton, tomato and other plants, some of which have entered the stage of practical application and produced certa...

AI Technical Summary

Problems solved by technology

The use of disease-resistant varieties and chemical fungicides are still the main methods of controlling rice blast at present. However, the existing problems are that rice varieties resistant to rice blast are relatively single, and the physiological races of rice blast fungus are complex and have certain variability. Pesticides have certain toxicity, pollute the environment, and long-term use of chemical pesticides will cause pathogenic bacteria to develop drug resistance

These problems limit the control of rice blast and become the main obstacle to the sustainable development of agriculture.

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