The invention provides a method for preparing
sapogenin through
chenopodium quinoa willdL seed coat and a separation and quantification method of the
sapogenin, and belongs to the technical field of
biological product processing. The method for preparing the
sapogenin through the
chenopodium quinoa willdL seed coat comprises the following steps that firstly, the
chenopodium quinoa willdL seed coat, complex
enzyme and deionized water are mixed and enzymolyzed to obtain enzymatic
hydrolysate; then the enzymatic
hydrolysate and ethyl
alcohol are mixed, chenopodium quinoa willdL
saponin in the enzymatic
hydrolysate is extracted through ultrasonic
waves,
solid-liquid separation is conducted, and a chenopodium quinoa willdL
saponin extracting solution is obtained; then the chenopodium quinoa willdL
saponin extracting solution and
hydrochloric acid are mixed and subjected to acidolysis to obtain acidolysis liquid; and finally, the acidolysis liquid is extracted through
petroleum ether to obtain extract liquor, and a
solvent in the extract liquor is removed through
evaporation, and the sapogenin is obtained. According to the method, the chenopodium quinoa willdL seed coat serves as a
raw material to prepare the sapogenin, the natural sapogenin
raw material can be provided for a professional pharmaceutical factory, the comprehensive
utilization rate and the economic added value of chenopodium quinoa willdL can further be increased, and reuse and
sustainable development of resources are facilitated. The invention further provides a method for simultaneous separation and quantification of
oleanolic acid and
hederagenin in the chenopodium quinoa willdL seed coat through UPLC-MS.