Feed additive produced from waste liquid and dregs in pharmaceutical factory and preparation method of feed additive
A feed additive and traditional Chinese medicine waste residue technology, applied in animal feed, animal feed, application, etc., can solve problems such as unstable effect, allergic reaction, drug residue, etc., to improve immunity, avoid negative effects, and reduce emissions.
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Embodiment 1
[0020] (1) Preparation of culture medium:
[0021] Peptone 3.7%, glucose 4%, salt 0.5%, liver ointment 0.5% kg, drinking water 90.3%. Put the above medium components (except glucose) in quantitative purified water and heat to dissolve them, add glucose, then adjust the pH to 6.5, dispense into 50ml test tubes with 1% calcium carbonate, 1000ml Erlenmeyer flask, 15000ml round In the bottom flask, aliquot 25ml for each test tube, 450ml for each Erlenmeyer flask, and 9000ml for each round-bottom flask. Sterilize at 121°C for 30 minutes, and leave to cool for later use.
[0022] (2) Preparation of liquid culture medium
[0023] The Enterococcus faecalis strain (No. 140623, Latin name: E. faecalis ) Inoculate the above-mentioned test tube culture medium, shake culture at 37°C for 24 hours, then inoculate into Erlenmeyer flask culture medium with an inoculum amount of 2%, shake culture at 39°C for 24 hours, and finally inoculate in round bottom flask culture medium, inoculate The amount ...
Embodiment 2
[0026] (1) Preparation of culture medium:
[0027] Peptone 3%, glucose 3%, salt 0.5%, liver ointment 0.5% kg, drinking water 92%. Put the above medium components (except glucose) in quantitative purified water and heat to dissolve them, add glucose, then adjust the pH to 6.5, dispense into 50ml test tubes with 1% calcium carbonate, 1000ml Erlenmeyer flask, 15000ml round In the bottom flask, aliquot 25ml for each test tube, 450ml for each Erlenmeyer flask, and 9000ml for each round-bottom flask. Sterilize at 121°C for 30 minutes, and leave to cool for later use.
[0028] (2) Preparation of liquid culture medium
[0029] The Enterococcus faecalis strain (No. 140623, Latin name: E. faecalis ) Inoculate in the above-mentioned test tube culture medium, shake culture at 39℃ for 20 hours, then inoculate into Erlenmeyer flask culture medium, the inoculation amount is 5%, shake culture at 39℃ for 24 hours, and finally inoculate into round bottom flask culture medium, inoculate The amount i...
Embodiment 3
[0032] (1) Preparation of culture medium:
[0033] Peptone 4%, glucose 4%, salt 1%, liver ointment 1% kg, drinking water 89%. Put the above medium components (except glucose) in quantitative purified water and heat to dissolve them, add glucose, then adjust the pH to 6.5, dispense into 50ml test tubes with 1% calcium carbonate, 1000ml Erlenmeyer flask, 15000ml round In the bottom flask, aliquot 25ml for each test tube, 450ml for each Erlenmeyer flask, and 9000ml for each round-bottom flask. Sterilize at 121°C for 30 minutes, and leave to cool for later use.
[0034] (2) Preparation of liquid culture medium
[0035] The Enterococcus faecalis strain (No. 140623, Latin name: E. faecalis ) Inoculate in the above-mentioned test tube culture medium, culture with shaking at 38°C for 23 hours, then inoculate into the Erlenmeyer flask culture medium, the inoculation amount is 4%, culture with shaking at 38°C for 22 hours, and finally inoculate the culture medium in a round bottom flask, Th...
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