Bacillus pumilus and application thereof

A technology of Bacillus pumilus and strains, applied in the field of applied microorganisms, can solve problems such as undiscovered public reports, and achieve the effects of promoting the healthy development of the cigarette industry, chemical quality coordination, and easy operation

Active Publication Date: 2010-09-08
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But through bibliographic search, find no public report identical with the content of the present invention

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  • Bacillus pumilus and application thereof
  • Bacillus pumilus and application thereof
  • Bacillus pumilus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Collection of test tobacco leaf samples

[0025] The tobacco leaf samples used in this study were all collected from the tobacco aging workshop of the raw material department of Hongyun Tobacco (Group) Co., Ltd. The specific information of the tobacco leaves is shown in Table 1.

[0026] Table 1. Tobacco leaf samples tested

[0027]

[0028] (2) The cultivation method of Bacillus pumilus Van35 (B. pumilus Van35).

[0029] 1) Test tube slant culture

[0030] A Luria-Bertani (LB) medium was used, and the formula was (gram / liter, g / v): 10 grams of peptone, 10 grams of sodium chloride, 5 grams of yeast extract, 20 grams of agar, and distilled water to a volume of 1000 milliliters. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate B. pumilus Van35 and culture at 37°C for 12 hours to obtain test tube species.

[0031] 2) Preparation of bacterial agent

[0032] A. Seed culture (LB medium), the formula is: 10 grams of peptone, 10 grams of sodium chlo...

Embodiment 2

[0063] (1) preparation of Bacillus pumilus Van35 bacterial agent: the liquid and solid culture of Bacillus pumilus Van35, the preparation method of bacterial agent is with embodiment 1.

[0064] (2) Tobacco leaf fermentation and analysis of main chemical components of tobacco leaves

[0065] Weigh 200g each of Baoshan Hongda B2F and C3F shredded tobacco that has been pre-balanced to 13% moisture, and spray 40mL of Van35 (B. pumilus Van35) bacterial agent, and the control group is sprayed with the same amount of sterile water. In this experiment, four kinds of tobacco leaves were treated, and three repetitions were set up. Then the treated tobacco leaf samples were put into 22°C, 60% constant temperature and humidity box to ferment for 20 days, the samples were taken to measure the main chemical components, and the measured data were averaged for analysis.

[0066] The test results of chemical components of tobacco leaves are as follows (Table 6 and Table 7). From the perspec...

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Abstract

The invention discloses bacillus pumilus and application thereof, which belong to the technical field of microbes. The produced strain of the invention is named Bacillus pumilus Van35 according to classification, and the preservation number of the strain is CGMCC No.3411. When the strain preparation is spayed, the soluble total sugar content and the reducing sugar content of tobaccos can be increased, while protein, total nitrogen and nicotine content are reduced. Therefore, specific values of the ratio of dextrose to nitrogen, the ratio of total sugar to betaine and the ratio of total nitrogen to total alkaloid tend to rise, the quality of chemicals is high and the quality of the smoke panel test is improved.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms, and more specifically, the invention relates to a bacillus pumilus. At the same time, it also relates to the new application of the bacillus pumilus in tobacco production. Background technique [0002] Tobacco is one of the important pillars of our country's finances. The State Tobacco Monopoly Administration clearly stated in Article 7 of the "Eleventh Five-Year" China Cigarette Science and Technology Development Outline that it is necessary to "overcome the key technologies that affect the aroma and quality of tobacco leaves" and narrow the quality gap with foreign tobacco leaves to improve domestic and foreign market competitiveness. . [0003] Tobacco leaf fermentation is the main technological link in the tobacco processing process. Improving the fermentation technology of tobacco leaves and improving the taste and quality of tobacco leaves has become an important means for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/18C12R1/08
Inventor 武怡段焰青者为杨金奎朱保昆张克勤李红娟
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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