The invention discloses a method for preparing vinegar with high amino acid and reducing sugar, and relates to a method for preparing vinegar. The method comprises the following steps of: mixing bran, wheat and pea meal to obtain a substrate, and inoculating aspergillus niger As3.3409 and aspergillus oryzae As3.951 to prepare a seed starter; mixing bran, wheat and pea meal to obtain a raw material, and inoculating the seed starter to prepare an uncooked material multi-strain daqu starter; stacking, loading into a pool, turning the starter, and forming the starter; and mixing sorghum flour, wheat flour and millet flour, adding water, adding the cooked material multi-strain daqu starter and a daqu starter into mixed staple food grain, stirring, performing alcoholic fermentation and acetic acid fermentation, sealing, after-ripening, smoking, pouring and drying the vinegar, and ageing to obtain a finished product. By the method, the content of the amino acid and the reducing sugar is greatly improved, the nutritional value of the vinegar is improved, and the irritation of acidity is reduced; and the starter is prepared from an uncooked material and has the advantages of complete enzyme system and multiple microbial fermentation populations of a biological fermentation agent.