17results about How to "Increased reducing sugar content" patented technology

The invention relates to bacillus velezensis for producing alpha-amylase from a tobacco source and application of the bacillus velezensis, and belongs to the technical field of microorganisms. The bacillus velezensis for producing the alpha-amylase from the tobacco source is A8-1, and the preservation number of the bacillus velezensis is CCTCC (China Center For Type Culture Collection) NO: M 20232632. The activity of the alpha-amylase produced by the bacillus velezensis A8-1 can reach up to 147.85 + / -0.05 U / mL at the high temperature of 70 DEG C through determination, and the content of tobacco starch in tobacco products can be effectively reduced when the alpha-amylase is applied to the tobacco products, so that the quality of the tobacco products is improved. On one hand, the quality, applicability and utilization rate of low-grade and low-grade tobacco leaves can be improved; on the other hand, the quality grade of tobacco leaves can be improved, and defects of high-quality tobacco leaves are overcome. Meanwhile, valuable microbial resources are provided for the tobacco industry, and a new technology is provided for promoting high-quality development of tobacco industry enterprises.

The present invention relates to the field of biomedicine technology, and specifically to the use of Laiyang pear residue polysaccharide in the preparation of drugs for treating and / or preventing ulcerative colitis. The in vitro digestion characteristics of Laiyang pear residue polysaccharide, its mechanism of action in improving ulcerative colitis and related signal pathways were studied. Laiyang pear residue polysaccharide was partially degraded under simulated salivary-gastrointestinal digestion conditions, resulting in a significant increase in reducing sugar content and a change in molecular weight. By constructing a dextran sulfate sodium (DSS)-induced ulcerative colitis mouse model, it was proved that Laiyang pear residue polysaccharide has an improving effect on UC; at the same time, it provides a basis for the research and development of Laiyang pear residue polysaccharide drugs for the treatment of ulcerative colitis.

This invention discloses a nicotine-resistant pectin lyase mutant and its application in tobacco leaf treatment. Using pectin lyase PL6 as the starting enzyme, this invention designs and optimizes mutation sites, designing single-site saturation mutations based on the two hydrogen bond formation sites between the enzyme and nicotine, Lys-107 and Asp-84, to construct mutants. The pectin lyase mutants D84A and K107R of this invention retain 83.63% and 81.30% of their enzyme activities at a 1% nicotine concentration, respectively. Spraying tobacco leaves with D84A and K107R enzyme solutions significantly improves tobacco leaf quality due to pectin degradation.

The invention relates to a millet steamed cake premix powder and a millet steamed cake and a preparation method thereof. The preparation method comprises the steps of soaking and grinding, heating gelatinization, enzymatic sterilization, preparing liquid cake seeds, preparing solid cake seeds, and drying and powdering. Yellow millet is introduced as the main raw material of steamed cake to prepare a new millet steamed cake product with unique millet flavor, mouthfeel and golden color. The use of mixed fermentation of yeast and lactic acid bacteria can avoid the single flavor of fermentation of a single strain, so that the flavor of the product is richer and mellower, the mouthfeel is softer, and the sourness and sweetness are appropriate. The millet steamed cake premix powder prepared by the invention is used to produce millet steamed cake, which eliminates multiple cumbersome processes, is simple and time-saving to operate, has good product storage stability, and is excellent in texture, specific volume, sensory organ and flavor, and is conducive to the industrialized production of millet steamed cake.

The invention provides an efficient buckwheat thick slurry preparation method which comprises the following steps: dispersing one or more of high-temperature-resistant alpha-amylase, medium-temperature alpha-amylase and saccharifying enzyme in water to prepare enzymatic hydrolysate; performing twin-screw extrusion on the enzymatic hydrolysate and the sieved buckwheat flour to obtain coarse buckwheat pulp; and grinding, centrifuging, homogenizing, sterilizing and filling the coarse buckwheat pulp colloid to obtain the dense buckwheat pulp. According to the preparation method, an extrusion technology and an enzymolysis technology are combined, the effect of enzyme on a substrate is enhanced through extrusion, enzyme activation is induced, the utilization rate of the enzyme is increased, the cost is reduced, and the stability, the sweetness, the liquefaction degree and the taste of the buckwheat pulp are remarkably improved; the double-screw extrusion technology is adopted, the process is simple, time and labor are saved, energy is saved, and continuous and efficient production of the buckwheat thick slurry is achieved; different types of enzyme preparations are compounded, so that the stability and sensory performance of the buckwheat thick slurry are remarkably improved, the content of reducing sugar in the thick slurry is remarkably increased, and the sweetness of the thick slurry is improved.

The invention discloses a nicotine-tolerant pectate lyase mutant and an application thereof in tobacco leaf treatment. According to the invention, pectin lyase PL6 is taken as a starting enzyme, mutation sites are designed and optimized, and single-site saturated mutation is designed according to two sites Lys-107 and Asp-84 of hydrogen bonds formed by enzyme and nicotine, so that a mutant is constructed. The enzyme activity of the pectate lyase mutants D84A and K107R still remains 83.63% and 81.30% under the condition that the nicotine concentration is 1%; d84A and K107R enzyme solutions are sprayed on the tobacco leaves, and the pectin is degraded, so that the quality of the tobacco leaves is remarkably improved.

The invention discloses a selenium-rich liquid fertilizer and a selenium-rich coriander planting method, and belongs to the technical field of selenium-rich crop planting, and the selenium-rich liquid fertilizer comprises a basic selenium-rich liquid fertilizer and a growth regulator. The selenium-rich basic liquid fertilizer and the growth regulator are cooperatively used and are sprayed in a specific growth period, so that the selenium content, the reducing sugar content, the total flavone content, the hydroxyl radical scavenging rate and other indexes in the coriander are remarkably improved, and the coriander is rich in nutritional ingredients and remarkable in selenium content. In addition, the growth regulator and the selenium-rich liquid fertilizer provide a new thought for planting of aromatic crops including coriander.

The present application provides a bacillus licheniformis strain HSCY3018, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCCM20221831, and the preservation date is November 28, 2022. The present application also provides the application of the HSCY3018 strain and a microbial inoculum thereof in food vinegar brewing. The HSCY3018 has high amylase and protease activities in food vinegar brewing, and can significantly improve the raw material utilization rate of food vinegar brewing. Compared with a control group, the starch utilization rate is increased by 58.50%, the lactic acid content in organic acid is increased by 72.95%, the total amount of amino acid is increased by 66.18%, the reducing sugar content is increased by 43.96%, and the total ester content is increased by 275.74%.

The invention discloses screening and application of streptomyces, and belongs to the technical field of microorganisms. According to the invention, a strain Streptomyces sp.G57 with pectinase production capability is obtained by utilizing specific plate screening with pectin as a substrate, so that the content of reducing sugar and the content of aroma substances in tea leaves are increased. The enzyme activity of pectinase in the strain fermentation liquor is 1.82 U.L <-1 >. After the Streptomyces sp.-G57 strain is applied to fermentation of low-quality tea, pectin is hydrolyzed, the content of reducing sugar is increased by 28.53%, volatile substances such as L-beta-pinene (resin aroma), ethyl hexanoate (fruity aroma) and beta-cyclocitral (herbal aroma and rose aroma) with flower and fruit aroma properties are generated, and the purpose of improving the sweetness and aroma of the tea is achieved.

The present invention belongs to the technical field of deep processing of peanuts, and specifically relates to a method and application thereof for simultaneously improving the starch hydrolysis rate and protein quality in hot-pressed peanut meal. The specific method is that the peanut meal is pretreated first: water is added after ultrafine grinding, the pH is adjusted to 2, and constant temperature extraction is performed; after extraction, the pH is adjusted to 6, high temperature α-amylase is added, constant temperature oscillation is performed for enzymolysis, the pH is adjusted to 4.2-4.7, the enzyme is inactivated, and centrifugation is performed; protein extraction: water is added to the precipitate after centrifugation, the pH is adjusted to 8-10, and the precipitate is obtained by centrifugation; the precipitate is repeatedly extracted once, the supernatant is combined and the pH value is adjusted, and the precipitate is collected by centrifugation again; it is washed with deionized water, the pH is adjusted to 7.0-7.2, and the peanut meal protein is dried to obtain. The method of the present invention combines pretreatment with enzyme treatment technology; on the one hand, it can improve the degree of starch hydrolysis and increase the content of reducing sugars; on the other hand, the protein purity, emulsification, emulsification stability and the content of amino acids of peanut meal protein are improved; and the added value of the product is greatly improved.

The invention belongs to the field of tobacco gene engineering, and particularly relates to a tobacco helicase gene and application thereof, the tobacco helicase gene is an RECQL1 gene, the sequence of the tobacco helicase gene is as shown in SEQ ID NO.1, and after the RECQL1 gene is translated, the sequence of a protein coded by the tobacco helicase gene is as shown in SEQ ID NO.3. A CRISPR / Cas9 editing carrier used for knocking out the RECQL1 gene is constructed through a CRISPR / Cas9 mediated gene editing technology, a tobacco plant with the RECQL1 gene knocked out is obtained after editing material creation and molecular detection and identification, and compared with a control tobacco plant, the obtained RECQL1 gene knocked out editing tobacco plant has the advantages that the yield of the tobacco plant is increased, and the yield of the tobacco plant is increased. And the contents of chlorogenic acid, total sugar and reducing sugar in the tobacco leaves in the mature period are obviously improved.

The invention relates to a method for preparing thick corn pulp through enzyme addition and extrusion. The method comprises the following steps: crushing corn to obtain corn flour; uniformly dispersing at least one of medium-temperature alpha-amylase, high-temperature alpha-amylase and saccharifying enzyme in purified water to prepare enzymatic hydrolysate; performing twin-screw extrusion on corn flour and the enzymatic hydrolysate to obtain corn coarse pulp; and grinding, centrifuging, homogenizing, sterilizing and filling the coarse corn pulp to obtain the thick corn pulp. According to the method, the extrusion technology and the enzymolysis technology are combined, the effect of the enzyme on the extrusion substrate is enhanced by utilizing the efficient activity of the extrusion induction enzyme, the method is efficient in short time and energy-saving, and the continuous production of the thick corn slurry can be realized. The stability, liquefaction degree, sweetness and sensory quality of the thick corn pulp are remarkably improved, no extra stabilizer or thickener needs to be added, the thick corn pulp is green and safe, the requirement of consumers for the taste of the thick corn pulp is met, and the consumer market of the thick corn pulp is expanded.

The invention provides a composite enzymatic hydrolysis preparation as well as a preparation method and application thereof in aquaculture, and particularly belongs to the technical field of aquaculture. The compound enzymolysis preparation is prepared from cellulase, xylanase, medium-temperature amylase, low-temperature amylase, saccharifying enzyme, yeast extract powder and auxiliary materials. After corn flour is subjected to enzymolysis by using the composite enzymolysis preparation, the content of reducing sugar in the corn flour can be rapidly increased, and after the composite enzymolysis preparation is applied to a culture water body, the carbon nitrogen ratio of the water body can be increased, growth of indigenous microorganisms is promoted, and the self-cleaning capacity of the water body is improved; meanwhile, the compound enzymolysis preparation has a remarkable decomposition effect on moss in aquaculture water, and has a potential application prospect in moss prevention and control in shrimp and crab culture.

A strain of Bacillus licheniformis, its screening method and application, in the field of microbial fermentation technology, wherein the Bacillus licheniformis is classified as Bacillus licheniformis ( Bacillus licheniformis BL-2501, with accession number CCTCC NO: M20251206, accession date May 28, 2025. In this invention, BL-2501, using corn stalks as a carbon source for fermentation, achieved degradation rates of 38.52% for NDF and 31.61% for ADF. During the fermentation of corn stalks to prepare feed, it effectively increased the crude protein content and reducing sugar content of the feed, and significantly improved the sulfur-containing amino acid content, with methionine and cysteine ​​reaching 5.88 mg / g and 0.73 mg / g, respectively, thus significantly improving the nutritional value of the feed.

This invention relates to the field of animal feed technology, specifically to a herbivore feed and its preparation method. The herbivore feed includes silage, wheat straw, corn flour, brewer's grains, beet pulp, wrapped corn silage, and soybean meal; the silage includes dried apple pomace, wheat straw, and hybrid Napier grass. The preparation method of this herbivore feed is simple. Adding dried apple pomace to the silage to adjust moisture content helps increase the reducing sugar content of the silage, thereby increasing foraging activity in ruminants in southern pastoral areas, mitigating heat stress in ruminants during summer, maintaining stable milk production in dairy cows, and increasing economic benefits.

This invention belongs to the field of bioengineering technology, specifically disclosing the application of xylanase AlXyn10A in the degradation of xylans containing complex side chains. The amino acid sequence of the xylanase AlXyn10A provided by this invention is shown in SEQ ID NO:1, and this enzyme is derived from *Aspergillus foetida*. Aspergillus leporis The specific activity of the pure enzyme reached 306.4 U / mg, and Co 2+ It exhibits significant activation and excellent hydrolytic performance. Structural analysis shows that this xylanase can simultaneously recognize both FAX3 and MeGA substrates, effectively degrading xylans with complex side chains. It has broad application prospects in various fields such as food, agricultural waste resource utilization, textiles, papermaking, and environmental remediation. Applying this xylanase to the pear juice preparation process can significantly increase the juice yield and reducing sugar content while effectively preserving ascorbic acid.

The invention belongs to the field of tobacco gene engineering, and particularly relates to a tobacco dehydrogenation diphosphate monoglyceride synthase gene and application thereof, the tobacco dehydrogenation diphosphate monoglyceride synthase gene for regulating and controlling the tobacco plant height and the tobacco leaf total sugar and reducing sugar content is an At5g58770 gene, and the sequence of the gene is shown as SEQ ID NO.1. After the At5g58770 gene is translated, the content of the tobacco leaf total sugar and reducing sugar in the tobacco leaf total sugar and reducing sugar in the tobacco leaf total sugar and reducing sugar in the tobacco leaf total sugar and reducing sugar in the tobacco leaf total sugar and reducing sugar in the tobacco leaf total sugar can be regulated and controlled. And the sequence of the protein coded by the gene is shown as SEQ ID NO.3. A CRISPR / Cas9 editing carrier used for knocking out the At5g58770 gene is constructed through a CRISPR / Cas9 mediated gene editing technology, and a tobacco plant with the At5g58770 gene knocked out is obtained after editing material creation and molecular detection and identification. Compared with a control tobacco plant, the editing tobacco plant with the At5g58770 gene knocked out has the advantages that the normal plant height and the topped plant height are remarkably reduced, and the content of total sugar and reducing sugar in tobacco leaves in the mature period is remarkably increased.