6results about How to "Increased reducing sugar content" patented technology

This invention discloses a nicotine-resistant pectin lyase mutant and its application in tobacco leaf treatment. Using pectin lyase PL6 as the starting enzyme, this invention designs and optimizes mutation sites, designing single-site saturation mutations based on the two hydrogen bond formation sites between the enzyme and nicotine, Lys-107 and Asp-84, to construct mutants. The pectin lyase mutants D84A and K107R of this invention retain 83.63% and 81.30% of their enzyme activities at a 1% nicotine concentration, respectively. Spraying tobacco leaves with D84A and K107R enzyme solutions significantly improves tobacco leaf quality due to pectin degradation.

The present application provides a bacillus licheniformis strain HSCY3018, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCCM20221831, and the preservation date is November 28, 2022. The present application also provides the application of the HSCY3018 strain and a microbial inoculum thereof in food vinegar brewing. The HSCY3018 has high amylase and protease activities in food vinegar brewing, and can significantly improve the raw material utilization rate of food vinegar brewing. Compared with a control group, the starch utilization rate is increased by 58.50%, the lactic acid content in organic acid is increased by 72.95%, the total amount of amino acid is increased by 66.18%, the reducing sugar content is increased by 43.96%, and the total ester content is increased by 275.74%.

The invention provides a composite enzymatic hydrolysis preparation as well as a preparation method and application thereof in aquaculture, and particularly belongs to the technical field of aquaculture. The compound enzymolysis preparation is prepared from cellulase, xylanase, medium-temperature amylase, low-temperature amylase, saccharifying enzyme, yeast extract powder and auxiliary materials. After corn flour is subjected to enzymolysis by using the composite enzymolysis preparation, the content of reducing sugar in the corn flour can be rapidly increased, and after the composite enzymolysis preparation is applied to a culture water body, the carbon nitrogen ratio of the water body can be increased, growth of indigenous microorganisms is promoted, and the self-cleaning capacity of the water body is improved; meanwhile, the compound enzymolysis preparation has a remarkable decomposition effect on moss in aquaculture water, and has a potential application prospect in moss prevention and control in shrimp and crab culture.

A strain of Bacillus licheniformis, its screening method and application, in the field of microbial fermentation technology, wherein the Bacillus licheniformis is classified as Bacillus licheniformis ( Bacillus licheniformis BL-2501, with accession number CCTCC NO: M20251206, accession date May 28, 2025. In this invention, BL-2501, using corn stalks as a carbon source for fermentation, achieved degradation rates of 38.52% for NDF and 31.61% for ADF. During the fermentation of corn stalks to prepare feed, it effectively increased the crude protein content and reducing sugar content of the feed, and significantly improved the sulfur-containing amino acid content, with methionine and cysteine ​​reaching 5.88 mg / g and 0.73 mg / g, respectively, thus significantly improving the nutritional value of the feed.

This invention relates to the field of animal feed technology, specifically to a herbivore feed and its preparation method. The herbivore feed includes silage, wheat straw, corn flour, brewer's grains, beet pulp, wrapped corn silage, and soybean meal; the silage includes dried apple pomace, wheat straw, and hybrid Napier grass. The preparation method of this herbivore feed is simple. Adding dried apple pomace to the silage to adjust moisture content helps increase the reducing sugar content of the silage, thereby increasing foraging activity in ruminants in southern pastoral areas, mitigating heat stress in ruminants during summer, maintaining stable milk production in dairy cows, and increasing economic benefits.

This invention belongs to the field of bioengineering technology, specifically disclosing the application of xylanase AlXyn10A in the degradation of xylans containing complex side chains. The amino acid sequence of the xylanase AlXyn10A provided by this invention is shown in SEQ ID NO:1, and this enzyme is derived from *Aspergillus foetida*. Aspergillus leporis The specific activity of the pure enzyme reached 306.4 U / mg, and Co 2+ It exhibits significant activation and excellent hydrolytic performance. Structural analysis shows that this xylanase can simultaneously recognize both FAX3 and MeGA substrates, effectively degrading xylans with complex side chains. It has broad application prospects in various fields such as food, agricultural waste resource utilization, textiles, papermaking, and environmental remediation. Applying this xylanase to the pear juice preparation process can significantly increase the juice yield and reducing sugar content while effectively preserving ascorbic acid.