Pretreatment method for improving saccharification efficiency of rape stalks

A technology of rape straw and saccharification rate, which is applied in biosynthesis, biofuel, fermentation, etc., can solve the problems of cellulose and hemicellulose consumption, the impact of fermentation process, serious environmental pollution, etc., and achieve the reduction of crystallinity and low equipment requirements , the effect of low reaction temperature

Inactive Publication Date: 2014-02-19
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of acid and alkali used in this method is large and the recovery rate is low, which seriously pollutes the environment and requires high equipment. Usually, a large amount of by-products are produced, which affects the subsequent fermentation process.
The biological method has simple equipment, low energy consumption, no pollution, and mild conditions, but one of the biggest disadvantages of the microbial treatment method is that the treatment cycle is long, and many microorganisms also consume part of cellulose and hemicellulose while decomposing lignin.

Method used

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  • Pretreatment method for improving saccharification efficiency of rape stalks

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Effect test

Embodiment 1

[0030] The air-dried rape stalks were coarsely chopped and crushed to 30-mesh powder, put into a 1000mL white glass bottle, and subjected to 60 Co-γ-ray irradiation, the dose is 800kGy, irradiated at natural room temperature, the radiation cobalt source intensity is 9.99×10 15 Bq, dose rate 2kGy / h. All irradiation pretreatment experiments were conducted in the Hunan Provincial Irradiation Center 60 Co-γ-ray irradiation device completed. Take 1 gram of the above irradiated powder, add 0.5% sulfuric acid solution according to the solid-to-liquid ratio of 1:20, stir evenly, treat in a water bath at 80°C for 1.5 hours, cool and filter, wash the residue with water until it is neutral, and then dry it at 50°C; Take 1 gram of the dried residue and add it to the citric acid-sodium citrate buffer solution with pH 5.0 at a solid-to-liquid ratio of 1:25, then add Novozymes cellulase 225FPU / g, shake at 50°C and 150rpm, and perform enzymatic hydrolysis for 72 Hours, the measured reducin...

Embodiment 2

[0032] The air-dried rape stalks were roughly chopped and crushed to 20-mesh powder, put into a 1000mL white glass bottle, and subjected to 60 Co-γ-ray irradiation, the dose is 1000kGy, irradiated at natural room temperature, the radiation cobalt source intensity is 9.99×10 15 Bq, dose rate 2kGy / h. All irradiation pretreatment experiments were conducted in the Hunan Provincial Irradiation Center 60 Co-γ-ray irradiation device completed. Take 1 gram of the above irradiated powder, add 1.1% sulfuric acid solution at a solid-to-liquid ratio of 1:15, stir evenly, treat in a water bath at 90°C for 2.0 hours, cool and filter, wash the residue with water until it is neutral, and then dry it at 60°C; Take 1 gram of the dried residue and add it to the citric acid-sodium citrate buffer solution with pH 4.8 at a solid-to-liquid ratio of 1:30, then add Novozymes cellulase 250 FPU / g, shake at 55°C and 140 rpm, and enzymatically digest 96 Hours, the measured reducing sugar content reache...

Embodiment 3

[0034] The air-dried rape stalks were coarsely chopped and crushed to 40 mesh powder, put into a 1000mL white glass bottle, and subjected to 60 Co-γ-ray irradiation, the dose is 600kGy, irradiated at natural room temperature, the radiation cobalt source intensity is 9.99×10 15 Bq, dose rate 2kGy / h. All irradiation pretreatment experiments were conducted in the Hunan Provincial Irradiation Center 60 Co-γ-ray irradiation device completed. Take 1 gram of the above irradiated powder, add 2.1% sulfuric acid solution at a solid-to-liquid ratio of 1:10, stir evenly, treat in a water bath at 70°C for 1.0 hour, cool and filter, wash the residue with water until it is neutral, and then dry it at 45°C; Take 1 gram of the dried residue and add it to the citric acid-sodium citrate buffer solution with pH 4.5 at a solid-to-liquid ratio of 1:20, then add Novozymes cellulase 200 FPU / g, shake at 50°C and 145rpm, and perform enzymatic hydrolysis for 48 Hours, the measured reducing sugar cont...

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Abstract

The invention relates to a pretreatment method for lignocellulose raw materials, in particular to a pretreatment method for improving the saccharification efficiency of rape stalks. The pretreatment method comprises steps as follows: the rape stalks are crushed into coarse powder; the coarse powder is subjected to irradiation treatment by a ray; the coarse powder after the irradiation treatment is added into a sulfuric acid solution for drying; and residues after drying are subjected to enzymolysis. According to the pretreatment method, the rich waste rape stalk resource in China is used sufficiently, and a new way is provided for comprehensive utilization of the rape resource and development of renewable energy resources; and further, the pretreatment method is little in pollution, high in enzymolysis efficiency, good in saccharification effect, low in cost and simple in operation.

Description

technical field [0001] The invention relates to a pretreatment method for lignocellulosic raw materials, in particular to a pretreatment method for improving the saccharification rate of rapeseed stalks. Background technique [0002] Rapeseed is a lignocellulosic material rich in cellulose, which belongs to the annual herbaceous plant, and is widely cultivated in the north and south of my country with high yield. Rapeseed straw can replace traditional starches and sugars as fermentation raw materials to prepare ethanol. At the same time, it is widely used in fields such as construction, breeding, and land improvement. Its ecological value and economic value are increasingly recognized by scientific and technological workers. Attention and attention. However, as a lignocellulosic raw material to prepare fuel ethanol, its degradation faces a problem, that is, natural plant fiber raw materials such as rapeseed are mainly composed of cellulose, hemicellulose and lignin, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCY02E50/16Y02E50/10Y02P20/59
Inventor 张春艳谭兴和熊兴耀苏小军
Owner HUNAN AGRICULTURAL UNIV
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