Special marine biological fertilizer for tobacco as well as preparation method and application of fertilizer

A technology of marine organisms and special fertilizers, applied in fertilization methods, applications, inorganic fertilizers, etc., to achieve the effects of improving the quality of tobacco leaves, enhancing the ability of disease resistance and promoting growth, and having a good market application prospect

Active Publication Date: 2015-06-24
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low boiling point of cadmium, most of the cadmium in cigarettes enters the smoke (about 70%), and the cadmium provided by smoking 40 cigarettes per day is equivalent to twice that of food, which poses a potential and huge health risk to both active and passive smokers

Method used

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  • Special marine biological fertilizer for tobacco as well as preparation method and application of fertilizer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Extraction process of exopolysaccharide of Bacillus floraceus SJ

[0026] Take the Bacillus japonica SJ strain (preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC No.7571), the colony shape is round, with neat edges, 4-6mm in diameter, and white mucus. Under laboratory conditions, it has obvious inhibitory effect on tobacco fusarium, tobacco red spot disease, tobacco black shank and other fungal diseases, and tobacco virus disease (TMV).

[0027] Preparation of seed culture solution: Inoculate the slant of the strain on the seed medium, the seed medium is: peptone 5-10g, beef extract 1-3g, sodium chloride 1-5g, ferric chloride 0.05-0.3g, distilled water 1000ml, pH6. 8~7.4. Cultivate at a temperature of 30-35° C. and a rotational speed of 170 rpm for 18-24 hours to obtain a seed culture solution.

[0028] Fermentation production: the seed culture solution is inoculated int...

Embodiment 2

[0034] Example 2, exopolysaccharides inhibiting effect on TMV

[0035]Inhibitory effect of extracellular polysaccharides on TMV: Take the extracellular polysaccharides of the strain SJ, dilute them 100 times, 500 times, 1000 times, 2000 times, mix them with 40 times the same volume of TMV virus juice for 15 minutes, inoculate Sansheng NN cigarettes, and Sterile water mixed with an equal volume of TMV was used as a control to detect its scab inhibition rate.

[0036] Table 1 Inhibitory effect of crude polysaccharide extracted from strain LJ-2 on TMV

[0037]

Embodiment 3

[0038] Embodiment 3, enteromorpha polysaccharide water retention, adsorption heavy metal ion experiment

[0039] 1) Determination of water retention of Enteromorpha polysaccharides

[0040] The water retention capacity of Enteromorpha polysaccharides was determined by calculating the evaporation rate of soil water. Take a cube with a length, width, and height of 10 cm and a volume of 1 decimeter as a container. The soil is ordinary loess. After drying at 105°C for 4 hours to constant weight, fill it into the container and compact it, and record the soil mass (Ms) Take tap water as contrast, make soil fully absorb water, write down used tap water quality (Mw); Respectively with the enteromorpha polysaccharide polysaccharide solution of 1 / 100,000 (A), 1 / 10,000 (B) concentration as experimental object, will The soil and exopolysaccharide solution of the same quality as the control were filled into empty containers, and placed at a constant temperature of 30°C for 3 to 15 days, r...

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Abstract

The invention provides a special marine biological fertilizer for tobacco as well as a preparation method and an application of the fertilizer. The special marine biological fertilizer for the tobacco is prepared from enteromorpha polysaccharides having an obvious promoting effect on the appearance and the inherent quality of the tobacco, and exopolysaccharides generated by a screened bacillus vallismortis SJ strain resistant to viral and fungal diseases, wherein the bacillus vallismortis SJ strain is collected in China General Microbiological Culture Collection Center with the collection number of CGMCC No.7571. The special marine biological fertilizer is capable of reducing the content of heavy metals in the tobacco leaves, increasing the total sugar and reducing sugar content of the tobacco leaves and enhancing the disease resisting and growth promoting ability of the tobacco. The marine biological fertilizer is applied to the tobacco and capable of achieving the purposes of environmental protection, high efficiency and new type; and besides, the marine biological fertilizer is capable of obviously improving the tobacco leaf quality, contributes for increasing the income of peasants and the local finance and taxation, and has a favorable market application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a special marine biological fertilizer for tobacco and its preparation method and application. Background technique [0002] Seaweed polysaccharide is the main active substance in seaweed, which can enhance crop resistance, enhance the osmotic pressure of cells by regulating cell osmosis, thereby enhancing the ability of cold resistance, drought resistance, and salt-alkali resistance; it can participate in the body's photosynthesis and indirectly improve the body's immunity It can prevent and control viral and fungal diseases, and has an anti-absorption effect on heavy metals and other toxic substances, effectively improving crop quality. As an additive, seaweed polysaccharide can enhance the stability and solubilization of the product, enhance the emulsification, water absorption and chelation of inorganic ions and heavy metals in the system. Polysaccharides are importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
CPCA01C21/00C05B7/00C05G3/00C05G3/80C05C3/00C05C9/00C05D1/02C05D9/02
Inventor 单俊伟翟熙伦王海华
Owner 青岛海大生物集团股份有限公司
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