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Preparation method of folium ginkgo biological feed additive

A technology of biological feed and ginkgo biloba, applied in animal feed, animal feed, application, etc., can solve the problems of low investment and weak technical force, and achieve increased reducing sugar content, improved nutritional value and biological activity, and improved transfer rate of flavonoids Effect

Active Publication Date: 2015-12-23
GUANGDONG JINXINNONG FEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the investment in the research and development of biological feed additives in my country is small and the technical force is weak, so that there are few biological feed additives with their own intellectual property rights and characteristics in my country.

Method used

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  • Preparation method of folium ginkgo biological feed additive
  • Preparation method of folium ginkgo biological feed additive
  • Preparation method of folium ginkgo biological feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The invention provides a preparation method of ginkgo biloba biological feed additive, which is mainly completed through the following processes:

[0035] 1) Preparation of seed medium

[0036] Glucose 20g, yeast extract 5g, peptone 5g, agar powder 10g, add water to boil, dilute to 1000mL. Sterilize at 121°C for 30 minutes.

[0037] 2) Preparation of solid fermentation medium

[0038] According to the mass content of Ginkgo biloba 91%, bran 4%, ammonium sulfate 5%, and the Cu with final concentration of 1.5mmol / L 2+ , 1.4mmol / LCa 2+ , 1.3mmol / LMn 2+ , Fe 2+ , Fe 3+ , 1mmol / L of Mg 2+ , Zn of 1.25mmol / L 2+ , moisture content 100%, sterilized at 121°C for 30min.

[0039] 3) Activation of strains

[0040] The original preserved Aspergillus niger strains in the refrigerator in the laboratory were taken out and inserted into the slant seed medium for activation. After the spores cover the slope, use sterile water to make a spore suspension, count with a microscope...

Embodiment 2

[0043] The optimization of embodiment 2 fermentation medium

[0044] 1) Selection of carbon source types

[0045] 2% of different carbon sources such as bran, cornmeal, glucose, sucrose, rice straw, rice bran, water-soluble starch and the like are respectively added into the basal medium for solid fermentation. Different carbon sources have different effects on the amount of reducing sugar produced by solid fermentation of Aspergillus niger from Ginkgo biloba leaves. The effects of different carbon sources on the content of reducing sugars produced are shown in Table 1 and figure 1shown.

[0046] Table 1 The impact of carbon source types on the content of reducing sugar produced

[0047] Type of carbon source

Reducing sugar content (mg / g)

bran

8.754

glucose

8.050

sucrose

8.153

soluble starch

6.985

cornmeal

7.964

the straw

7.534

rice bran

7.053

[0048] Add 2% bran, cornmeal, gl...

Embodiment 3

[0076] The optimization of embodiment 3 fermentation conditions

[0077] 1) The influence of water content

[0078] The water content of the culture medium is adjusted to 60%, 80%, 100%, 120%, 140%, 160%, and 180%, respectively, and the fermentation is carried out. The water content of the fermentation medium is different, and the content of reducing sugar produced by solid fermentation is different, such as Image 6 shown.

[0079] The water content of the culture medium is adjusted to 60%, 80%, 100%, 120%, 140%, 160%, and 180%, respectively, and the fermentation is carried out. The results show that when the water content is 60%-140%, the content of reducing sugar increases with the increase of water content, reaches the maximum when the water content is 140%, and decreases with the increase in the water content of 140%-180%. . When the water content is 140%, it reaches the maximum, and the content of reducing sugar is the highest.

[0080] 2) The influence of fermentat...

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Abstract

The invention discloses a preparation method of folium ginkgo biological feed additive. Solid fermentation is conducted under appropriate nutrient conditions and cultivation conditions with folium ginkgo as the main solid fermentation matrix and aspergillus niger as the bacterial strain, and the biological feed additive is obtained after drying and smashing; the biological feed additive contains various bioactive substances such as flavonoids compounds, and the organism immunity and oxidation resistance of animals can be improved. Meanwhile, the nutritional value and biological activity of the biotransformed product are greatly improved, and therefore a foundation is laid for deep research and development of the composite multifunctional folium ginkgo biological feed additive.

Description

technical field [0001] The invention belongs to the technical field of biological feed development, and in particular relates to a preparation method of a ginkgo biloba biological feed additive. Background technique [0002] Ginkgo biloba L. is a special fruit tree and garden plant in my country. Ginkgo is not only edible, but also medicinal. Ginkgo biloba contains a large amount of flavonoids, and its medicinal functions are mainly to treat coronary arteriosclerosis, cerebrovascular disease, angina pectoris, lower cholesterol, lower blood pressure, and inhibit certain bacteria and fungi. At present, there have been many reports on the development and utilization of ginkgo at home and abroad, such as ginkgo tea, ginkgo drinks, and ginkgo health products. With the improvement of consumers' requirements for the quality of animal products, the application of chemical feed additives such as hormones for disease prevention and growth promotion and antibiotics that are prone to ...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/16
Inventor 唐仕荣高兆建陈尚龙宋慧刘全德刘恩岐吴玉新
Owner GUANGDONG JINXINNONG FEED CO LTD
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