Method for promoting enzymolysis of cellulose

A cellulase and cellulose technology, which is applied in the field of cellulose enzymolysis, can solve the problems of low cellulose enzymolysis efficiency and low fermentable sugar concentration, and achieves the effect of improving production process, obvious effect and expanding varieties.

Inactive Publication Date: 2016-07-06
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low enzymatic hydrolysis efficiency of cellulose and low concentration of fermentable sugars existing in the current enzymatic hydrolysis process, the object of the present invention is to provide a method using 1-hexadecylpyridinium chloride and 1,2-propanediol as enzymes. Method for improving cellulose enzymatic hydrolysis by using hydrolysis aids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Add 4 parts by mass of 1-hexadecylpyridinium chloride salt and 1 part by mass of 1,2-propanediol to 90 parts by mass of citric acid-sodium citrate buffer solution with a pH of 5, and then add 8 FPU / g cellulase, After fully stirring evenly, 5 parts by mass of cellulose were added and reacted at a temperature of 50° C. for 45 hours to obtain a reducing sugar hydrolyzate containing 55.4%.

Embodiment 2

[0017] 6 parts by mass of 1-hexadecylpyridinium chloride salt and 2 parts by mass of 1,2-propanediol were added to 86 parts by mass of citric acid-sodium citrate buffer with a pH of 5.5, and then 9 FPU / g cellulase was added, After fully stirring evenly, 6 parts by mass of cellulose were added and reacted at a temperature of 50° C. for 50 hours to obtain a reducing sugar hydrolyzate containing 65.3%.

Embodiment 3

[0019] 8 parts by mass of 1-hexadecylpyridinium chloride salt and 3 parts by mass of 1,2-propanediol were added to 83 parts by mass of citric acid-sodium citrate buffer with a pH of 5.3, and then 10 FPU / g cellulase was added, After fully stirring evenly, 6 parts by mass of cellulose were added, and reacted at a temperature of 50°C for 55 hours to obtain a reducing sugar hydrolyzate containing 70.5%.

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Abstract

The invention discloses a method for promoting enzymolysis of cellulose. The method comprises the following steps: (1) adding 1-hexadecyl chloropyridine salt and 1,2-propylene glycol into a citric acid-sodium citrate buffer solution with a certain pH value; (2) adding cellulase into the solution obtained in the step (1); and (3) adding cellulose into the solution obtained in the step (2), and reacting at 40-60 DEG C for 24-72 hours, so as to obtain hydrolysate containing reducing sugar. According to the method provided by the invention, a method for promoting the enzymolysis of cellulose by virtue of 1,2-propylene glycol and an ionic liquid type cationic surfactant, namely 1-hexadecyl chloropyridine salt is disclosed for the first time, the content of reducing sugar is high, the production process is simple, and the environmental pollution is avoided; and the method is a safe production process beneficial to the improvement of the enzymolysis effect of cellulose.

Description

technical field [0001] The invention belongs to the field of cellulose enzymolysis, and in particular relates to a method for improving cellulose enzymolysis by using 1-hexadecylpyridinium chloride salt and 1,2-propanediol as enzymolysis auxiliary agents. Background technique [0002] Cellulose is one of the most abundant biomass in nature, accounting for 50% of the world's total biomass, including agricultural and forestry waste, waste paper, energy crops, etc., but most of it is not used and discarded at present, and the carbohydrate content is as high as 75%, which can serve as an important source of fermentable sugars to produce liquid fuels and other chemicals. Therefore, using cellulosic biomass as raw material and converting it into biomass energy that can replace fossil energy is one of the effective ways to alleviate the energy crisis and environmental pollution, and it will not endanger the world's food security while developing biofuels. With good development pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/02
CPCC12P19/02C12P19/12C12P19/14
Inventor 周雅文徐宝财韩富张桂菊赵莉刘红芹景姝王姗
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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