Method for degrading cassava starch by pullulanase synergistic dual enzymatic method

A technology of pullulanase and tapioca starch, which is applied in the direction of fermentation, etc., can solve the problems of the degree of hydrolysis and glucose yield, and achieve the effects of improving the degree of hydrolysis and glucose yield, accelerating the saccharification process, and improving the saccharification efficiency

Active Publication Date: 2010-08-25
云南万芳生物技术有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

(Wen Qibiao, Chen Ling, Luo Faxing. Grain and Feed Industry [J], 1997, (12): 33-34) But in fact, the dextrin and short-chain substrates produced by α-amylase after gelatinization first have great influence on saccharification The action point of the process has encapsulation and feedback inhibition, which inevitably has an impact on the degree of hydrolysis and glucose yield; however, since the starch granules formed by hydrolyzing the starch granules formed by heat absorption and swelling without adding pullulanase before starch gelatinization, the liquefaction still needs to maintain a temperature of 90 ℃

Method used

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  • Method for degrading cassava starch by pullulanase synergistic dual enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Adjust the tapioca starch and water into a starch paste at a ratio of 1:3, first add pullulanase, heat it in a water bath to a temperature of 60°C under stirring, and keep it for 30min; then raise the temperature to 70°C, add α- Amylase, keep for 30min; finally lower the temperature to 60°C, add glucoamylase, keep for 30min.

Embodiment 2

[0018] Example 2: Adjust the tapioca starch and water into a starch paste in a ratio of 1:3, heat it in a water bath to a temperature of 70°C under stirring, add amylase and pullulanase at the same time, and keep it for 30min; then lower the temperature to 60°C, Add glucoamylase and keep for 30min.

Embodiment 3

[0019] Example 3: Adjust the tapioca starch and water into a starch paste in a ratio of 1:3, heat it in a water bath to a temperature of 70°C under stirring, first add amylase, and keep it for 30 minutes; then lower the temperature to 60°C, add glucoamylase and common Lulanase, keep for 30min.

[0020] Table 1: Comparison of the order of addition of pullulanase, amylase, and glucoamylase to hydrolyze tapioca starch

[0021]

[0022] Table 2: The effect of the addition of pullulanase on the reducing sugar content of the system

[0023]

[0024]

[0025] It can be seen from the table that the content of reducing sugar in Experiment 1, where pullulanase was added first, was higher than that of other methods, and the content of reducing sugar in the system increased significantly.

[0026] (2) Dose-effect effect of pullulanase on cassava starch hydrolysis

[0027] According to the above experimental results, it is further adopted to add pullulanase to pretreat th...

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Abstract

The invention provides a method for degrading cassava starch by a pullulanase synergistic dual enzymatic method, and belongs to a method for separating starch and reducing sugar by using enzymes. The method comprises the following steps of: adding pullulanase, and then adding amylase and maltogenic amyiase to degrade the cassava starch, wherein dosages of the enzymes are: 10u of alpha amylase per gram of cassava starch, 200u maltogenic amyiase per gram of cassava starch and 20 to 40u of pullulanase per gram of cassava starch. When the dosage of the pullulanase is 30u per gram of cassava starch, the available reduced sugar content of the system is the highest. The method improves the reduced sugar content of the potato starch, increases hydrolysis rate, increases yield, and has an active significance for advancing the industrialization of the second-generation biological fuel of ethanol.

Description

technical field [0001] The invention belongs to a method for separating starch-reducing sugars by using enzymes. Background technique [0002] To promote the transformation of fuel ethanol production from the use of grain materials such as corn and wheat to the production of non-food crops such as cassava, sorghum, and sugarcane, it is necessary to improve the efficiency of enzymes or other saccharification agents to decompose non-food crop raw materials. Tapioca starch is one of the starch raw materials commonly used to produce fuel ethanol at present. It contains up to 85% amylopectin. The branched chain is a dendritic macromolecule formed by α-1,6-glucosidic bonds that only account for 4% to 5% of cassava starch, and has a certain influence on its saccharification. Pullulanase is a debranching enzyme that can hydrolyze starch and its hydrolysis products, such as α-1,6-glucosidic bonds in dextrin, and has good thermal stability and acid resistance. "The effect of pullula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/16
Inventor 尹芳韩伟刘士清黄文荣柳静王金浩
Owner 云南万芳生物技术有限公司
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