Preparation method for compound enzyme system for high-efficiency degradation for lignocellulose and application thereof

Abstract

The invention discloses a preparation method for a compound enzyme system for high-efficiency degradation for lignocellulose and an application thereof. The preparation method comprises the following steps of: step 1, activating Trichoderma reesei strains or the derivative strains thereof; step 2, preparing spore suspension from the activated Trichoderma reesei strains or the derivative strains thereof, inoculating into a seed culture medium, and culturing for 24-36 hours in a shaking manner on a shaking table with a rotational speed of 170-200 r / min and at a temperature of 28-30 DEG C; and step 3, inoculating the seed culture medium inoculated with the Trichoderma reesei strains or the derivative strains thereof into a fermentation culture medium using microcrystalline cellulose and corncobs as a carbon source in a mixing ratio of 1:4, and performing fermentation tank culture. The compound enzyme system is used for treating corncobs, gas-detonating straw and the corn straw pre-treated by NaOH at a normal temperature. Via the method disclosed by the invention, the saccharification and hydrolysis capacities of the compound enzyme system are enhanced, the preparation cost of enzymes is reduced, and the pollution to environment is low.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a preparation method and application of a composite enzyme system for efficiently degrading lignocellulose. Background technique [0002] With the depletion of non-renewable fossil energy, more and more researches are focused on the development and utilization of new energy. Biomass energy is favored due to its abundant sources, low cost and renewable nature. As the most abundant renewable resource on earth, cellulose provides a rich source of biomass energy. According to reports, the total amount of cellulose produced by photosynthesis in the world every year can reach 10 11 Ton. However, cellulose is a linear high-molecular polymer composed of glucose molecules bound by 1,4-glycosidic bonds. Cellulose molecules are difficult to be utilized directly and must be degraded into small molecular sugars before they can be utilized. Cellulose can be degraded directly by acid, alkali ...

AI Technical Summary

Problems solved by technology

The ventilation effect of solid-state fermentation is not very good, the operability is poor, and it is not suitable for industrial scale-up

The carbon source used for enzyme production under liquid fermentation conditions is microcrystalline cellulose or pulp. Although the enzyme activity is high, the cost is high, the environment is seriously polluted, and it is not suitable for large-scale production; there are also cheap raw materials such as bran, straw and wheat. Straw, but the enzyme activity is low; there is also acid-treated corncob residue as raw material for fermentation, because the residue after acid treatment contains toxic by-products such as furfural, which can inhibit the growth of bacteria, so the enzyme activity is low

None of the existing production methods for cellulase can meet the requirement of producing a relatively complete enzyme system while ensuring high enzyme activity.

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