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Total coptis alkaloid preparation method

A technology of total alkaloids and coptis, applied in the direction of organic chemistry, can solve the problems of time-consuming concentration, large volume of decoction, and high impurity content, and achieve the effect of large sample loading, removal of impurities, and shortening the concentration process

Inactive Publication Date: 2006-02-08
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems of large volume of water decoction, time-consuming concentration and high impurity content when extracting medicinal materials such as Coptidis Rhizoma, and adopts styrene-type macroporous resin to prepare Coptis Rhizoma total alkaloids. The specific content is as follows:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Weigh 350g of Coptidis Rhizoma that has been properly crushed, and decoct twice with 9 and 6 times the amount of water respectively. -1 , add the supernatant to the pretreated DS-401 resin column (5.1×75cm glass column), wash with water, elute with 20% aqueous ethanol, concentrate the aqueous ethanol eluate to precipitate a large amount of alkaloid precipitation , and dried under reduced pressure to obtain 23g. With berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride, and coptisine sulfate as reference substances, the total alkaloid HPL of Coptidis Rhizoma (, the measurement results are shown in Table 1, and the known alkaloid content in the extract is 40.26%.

[0018] sample

Embodiment 2

[0020] Weigh 500g of Coptidis Rhizoma that has been properly crushed, and decoct it in water for 3 times with 9, 6, and 6 times the amount respectively, and the decoction is centrifugally settled, and the flow rate of sample loading and cleaning is controlled at 10mL min -1 , control the flow rate, add the supernatant to the pretreated DA-201 resin column (8.0×96cm glass column), wash with water, elute with 30% aqueous ethanol, and concentrate the aqueous ethanol eluent to precipitate a large amount of biological Alkali precipitation, drying that is 35.421g. The total alkaloid content of the extract is shown in Table 2.

[0021] sample

Embodiment 3

[0023] Weigh the Phellodendron Phellodendri that has been properly crushed, decoct twice with 9 and 6 times the amount of water respectively, filter the decoction, and control the flow rate of loading and cleaning to 15mL min -1 , control the flow rate and add the supernatant to the pretreated D-101-I type resin column (8.0 × 96cm glass column), wash with water, and elute with 30% aqueous ethanol, and the aqueous ethanol eluent Concentrate to precipitate a large amount of alkaloid precipitate, and dry under reduced pressure. The total alkaloid content of the extract is shown in Table 3.

[0024] sample

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PUM

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Abstract

Disclosed is a process for preparing total coptis root alkaloids through a novel phenylethene type microporous resin, the process consists of weighing right amount of disintegrated coptis medicinal materials, grilling with water, charging the supernatant onto pretreated resin column, water scrubbing, eluting with water-containing ethanol, concentrating the eluent to obtain precipitation of alkaloids, finally drying. HPLC detection shows that the content of total coptis root alkaloids is 25-100%.

Description

technical field [0001] The invention relates to a method for extracting and separating effective parts of traditional Chinese medicines, in particular to a method for extracting and separating total alkaloids of effective parts from Chinese medicine Coptis chinensis. Background technique [0002] Coptis chinensis (RHIZOMA COPTIDIS) is the dried rhizome of Coptis chinensis, Coptidis triangularis or Yunlian, which is recorded in the 2005 edition of Chinese Pharmacopoeia. ) commonly used drugs, mainly containing original berberine-type alkaloids such as berberine, palmatine, jatrorrhizine, coptisine and other alkaloid components. [0003] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature. They are the active ingredients of many Chinese herbal medicines. They often exist in the form of cations under neutral and acidic conditions, so they can generally be separated and enriched from the extract with cation exchange resins. , if the to...

Claims

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Application Information

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IPC IPC(8): C07D455/03
Inventor 魏英勤班青
Owner QILU UNIV OF TECH
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