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Method for extracting suaveolic acid A

A technology of behenic acid and behenic acid, which is applied in the production of steroids, bulk chemicals, organic chemistry, etc., can solve the problems such as no reports on the production process of preparing behenic acid A, and achieve easy large-scale production, high product purity and high quality. large sample size

Inactive Publication Date: 2013-01-23
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the production process for preparing behenic acid A

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use the aboveground part of the head-shaped mountain incense as raw material, cut it into sections of about 1 cm, put it into a high-speed tissue grinder, keep it at a speed of 18,000 rpm for 5 minutes, pass it through a 60-mesh sieve for later use, weigh 1 kg, and weigh 1 kg according to the ratio of material to liquid: 1: After adding 85% ethanol aqueous solution at a ratio of 18 (g / ml), add 0.5% cellulase, and enzymatically hydrolyze for 20 minutes under the conditions of ultrasonic power 500W and 41°C to obtain enzymatic hydrolysis raw materials, which are put into the extraction kettle for supercritical fluid Extraction, pumping CO into the extraction kettle 2 And entrainer, entrainer is 95% ethanol aqueous solution, consumption is 1ml / g crude drug, CO 2 The flow rate is 3ml / g crude drug / min, the extraction pressure is 28MPa, the extraction temperature is 55°C, the extraction time is 3h, the analysis pressure is 6MPa, the analysis temperature is 28°C, the extract ...

Embodiment 2

[0022] Use the aboveground part of the head-shaped mountain incense as raw material, cut it into sections of about 1 cm, put it into a high-speed tissue grinder, keep it at a speed of 18,000 rpm for 5 minutes, pass it through a 60-mesh sieve for later use, weigh 1 kg, and weigh 1 kg according to the ratio of material to liquid: 1: Add 85% ethanol aqueous solution at a ratio of 12 (g / ml), then add 0.5% cellulase, and perform enzymolysis at 500W ultrasonic power and 41°C for 20 minutes to obtain enzymatic hydrolysis raw materials, which are put into the extraction kettle for supercritical fluid Extraction, pumping CO into the extraction kettle 2 And entrainer, entrainer is 85% ethanol aqueous solution, consumption is 1ml / g crude drug, CO 2 The flow rate is 2ml / g crude drug / min, the extraction pressure is 42MPa, the extraction temperature is 35°C, the extraction time is 1.5h, the analysis pressure is 8MPa, the analysis temperature is 28°C, the extract is collected, the obtained ...

Embodiment 3

[0024] Use the aboveground part of the head-shaped mountain incense as raw material, cut it into sections of about 1 cm, put it into a high-speed tissue grinder, keep it at a speed of 18,000 rpm for 5 minutes, pass it through a 60-mesh sieve for later use, weigh 1 kg, and weigh 1 kg according to the ratio of material to liquid: 1: Add 85% ethanol aqueous solution at a ratio of 15 (g / ml), then add 0.5% cellulase, and perform enzymolysis for 15 minutes under the conditions of ultrasonic power 500W and 41°C to obtain enzymatic hydrolysis raw materials, which are put into the extraction kettle for supercritical fluid Extraction, pumping CO into the extraction kettle 2 And entrainer, entrainer is 90% ethanol aqueous solution, consumption is 1ml / g crude drug, CO 2 The flow rate is 3.5ml / g crude drug / min, the extraction pressure is 45MPa, the extraction temperature is 55°C, the extraction time is 1h, the analysis pressure is 7MPa, the analysis temperature is 36°C, the extract is col...

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PUM

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Abstract

The invention discloses a method for extracting suaveolic acid A. The method is characterized by comprising steps as follows: getting the aboveground part of head-shaped suaveolens as the raw material; crushing the tissue, and carrying out enzymolysis by virtue of cellulose, so as to obtain the raw material subjected to enzymolysis; carrying out supercritical CO2 extraction; separating and collecting to obtain triterpenes extractive; then purifying by high-pressure silica gelcolumn chromatography; collecting fraction of the suaveolic acid A; concentrating in a pressure reducing way; and recrystallizing to obtain the suaveolic acid A. The method disclosed by the invention is simple in operation, suitable for large-scale production, low in energy consumption, low in pollution and short in production period, and is an environment-friendly production technology.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine chemistry and relates to a method for extracting behenic acid A. Background technique [0002] Asprellic acid A (Asprellic acid A), the molecular formula is C 30 h 48 o 5 , molecular weight 488.71, colorless prism, mp.298-304℃. Behenic acid A is from the labiatae (Labiatae) capitula Hyptis capitata Jacq. A triterpenoid isolated from aerial parts. Pharmacological studies have shown that behenic acid A has cytotoxic activity against A-549, HCT-8, and P388. Its ED50 (μg / ml) is 5.9, 4.2, 6.7 in turn. [0003] In the prior art, there is no report on the production process for preparing behenic acid A. Contents of the invention [0004] The purpose of the present invention is to provide a method for extracting behenic acid A. [0005] To achieve the above object, the present invention adopts the following technical solutions: [0006] (1) Cut the above-ground part of Cinn...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCY02P20/54
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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