EST-SSR molecular marker developed based on lycoris aurea transcriptome sequence and application thereof

A technology of transcriptome sequence and labeling, which can be used in recombinant DNA technology, DNA/RNA fragments, determination/inspection of microorganisms, etc., and can solve problems such as non-specific molecular markers.
CN108359740AInactive Publication Date: 2018-08-03INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Publication Date
2018-08-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a set of EST-SSR molecular marker developed based on a lycoris aurea transcriptome sequence. The EST-SSR molecular marker comprises 20 pairs of primers, wherein nucleotide sequences are as shown in a sequence table SEQ ID NO. 1-40. The 20 pairs of EST-SSR marked primers are designed according to the transcriptome sequence, are proved to be definite SSR markers through genome DNA amplification and sequencing, have advantages of rich polymorphism, stable amplification, good repeatability, codominance and convenience in detection and the like, and can better distinguish the diversity of genetic germplasm of lycoris aurea. The set of markers fills up the lack of specific codominant markers of lycoris aurea, and can be applied to fields such as genetic diversity of germplasm resources, varietal purity identification, variety genetic lineage analysis and assisted breeding of lycoris aurea, thereby greatly promoting research, application and development of germplasm resources of lycoris aurea.
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Description

technical field

[0001] The invention relates to molecular marker technology, in particular to 20 EST-SSR molecular markers and applications thereof. Background technique

[0002] It is a perennial herb of Amaryllidaceae (Amaryllidaceae), which has important medicinal value and ornamental value. It not only contains lycorine, galanthamine, narciclasine and other amaryllidaceae alkaloids with specific pharmacological activity, but also has important ornamental value. Observable leaves. However, there are great differences in the germplasm resources of different sources, not only in the external morphology and karyotype, but also in the alkaloid content. The use of molecular markers to carry out the fingerprint analysis of Kudixiao germplasm resources can not only investigate the diversity of Kudixiao germplasm resources, but also has important significance for the efficient utilization and development of Kudixiao germplasm resources in the future, such as the high alkaloid c...

Claims

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