25results about How to "Yield is not affected" patented technology

The invention discloses an application of an FgGCV1 gene and a knockout mutant thereof in prevention and control of fusarium graminearum, and belongs to the technical field of microorganisms. The invention discloses for the first time that after the FgGCV1 gene is knocked out, generation of ascospores in the sexual reproduction process of fusarium graminearum can be inhibited, the fusarium graminearum is prevented and controlled, wheat scab is prevented and controlled, and the nucleotide sequence of the FgGCV1 gene is as shown in SEQ ID NO.1. The FgGCV1 gene deletion mutant specifically regulates and controls maturation of ascospores in the sexual reproduction process of fusarium graminearum, and the ascospores serve as an important primary infection source of wheat scab, so that the transmission path of the wheat scab can be blocked after the FgGCV1 gene is knocked out, and the FgGCV1 gene deletion mutant plays an important role in fusarium graminearum prevention and control and green ecological prevention and control; the method is also an important resource for researching the sexual reproduction process of filamentous ascomycetes.

The invention belongs to the field of molecular genetics, and particularly relates to a method for reducing the plant height of corn. The invention provides a method for reducing the corn plant height through precise point mutation.

The invention discloses a method for creating cadmium-resistant rice with low grain cadmium accumulation and application. The rice oshma3oslct1 double mutant expressing the Cd binding autophagy adapter protein AIM-SpMTL-GFP is constructed through CRISPR-Cas9, so that the transfer of Cd from xylem to phloem and the accumulation of Cd in root vacuoles are reduced, meanwhile, the Cd binding autophagy adapter protein AIM-SpMTL-GFP can also accumulate cadmium in protoplast vacuoles of leaves, the yield and quality of rice grains are not influenced, and the yield and quality of the rice grains are greatly improved. Cd accumulation of grains is reduced, and Cd is enriched into straws at non-edible parts, so that industrial treatment is facilitated. The cadmium-resistant rice with low grain cadmium accumulation can reduce cadmium pollution in a food chain, so that the harm of cadmium to a human body is reduced.

The invention discloses a novel single-cell CRISPR screening library. The invention provides a double-gRNA expression cassette, a double-gRNA expression cassette vector for single-cell CRISPR (clustered regularly interspaced short palindromic repeats) screening and a construction method of a single-cell CRISPR screening library. According to the CRISPR screening library constructed by the method, the single-cell CRISPR screening range can be expanded from a protein coding gene to ncRNA, and the ncRNA can be knocked out.

The present application relates to the technical field of genetic engineering, in particular to a rice G1 gene mutant g1-1437, its molecular marker and application. The rice G1 gene mutant g1-1437 provided by the present application takes rice G1 gene as reference sequence, the nucleotide sequence of the rice G1 gene is shown as SEQ ID NO. 8; the gene mutant g1-1437 comprises a mutation site that base C at the 112th position is mutated into base T. The mutation causes the chaff homologous transformation of rice into glume shell substances, which can be used as a phenotype screening marker of transgenic rice. The present application further provides a molecular marker identification method of the mutant, which has a good application prospect in the transference of new germplasm.

This invention discloses the application of the OsCAD3 protein and its encoding gene in regulating resistance to bacterial blight in rice, relating to the field of plant genetic engineering. The amino acid sequence of this gene protein is shown in SEQ ID NO.3. Cloning the gene editing site sequence, constructing a gene editing recombinant vector, and transforming it into rice can improve rice resistance to bacterial blight and ensure stable rice yield. The OsCAD3 gene editing vector constructed in this invention and the new OsCAD3 allele formed through genome editing can be applied to rice breeding for resistance to bacterial blight. Constructing an overexpression recombinant vector of the above gene coding region sequence and transforming it into rice can reduce rice resistance to bacterial blight and ensure stable rice yield. The above gene overexpression vector can be used in rice breeding to regulate bacterial blight. This invention can be used in the fields of rice germplasm resource improvement and genetic breeding, and is of great significance for breeding new rice varieties resistant to bacterial blight.

The utility model discloses a kind of dynamic wind-resistant flexible photovoltaic support, including support, load cable and photovoltaic panel. Photovoltaic panel is ridden on the load cable's cable sleeve by the back of ∩ shape or annular saddle, forms hinged non-fixed connection, can independently rotate around load cable. Each photovoltaic panel is configured with the buffer reset mechanism of spring and / or lever composition, spring has preset yield point, maintains photovoltaic panel stationary power generation when breeze, buffers swing amplitude and automatically resets when strong wind. The utility model uses dynamic wind-resistant route, wind resistance is strong, span is large, pile foundation is less, applicable to agricultural light complementary scene, and the shadow of its fast movement can stimulate crop growth.

The invention relates to the technical field of plant genetic engineering and molecular breeding, in particular to application of CmBt and CmBr genes in regulation and control of bitter taste of muskmelon fruits. Physical interaction exists between muskmelon fruit bitter taste regulation factors CmBt and CmBr proteins, and expression of a bitter taste synthesis key gene CmBi is synergistically activated; the CmbtCmbr double mutants are constructed through a gene editing technology, it is proved that double-gene knockout has a synergistic effect, and the content of cucurbitacine B in all tissue of muskmelon can be more remarkably reduced; field trials show that under induction of forchlorfenuron, bitter taste of fruits of the CmbtCmbr double mutant completely disappears, the effect of the CmbtCmbr double mutant is remarkably superior to that of a single-gene mutant, and quality characters such as fruit weight and sugar degree are not negatively influenced; effective gene resources and germplasm materials are provided for solving the industrial problem that fruits become bitter due to the fact that plant growth regulators are used in muskmelon production.

The invention provides a method for controlling slag overflow in a ladle during aluminum alloying of 38CrMoAl steel, which specifically comprises the following steps: adjusting the composition of alloy elements other than Al element to within the range of process requirements in a refining furnace at a later stage, and adjusting the argon flow rate of the ladle to (100-150) NL / min after the adjustment; immediately increasing the argon flow rate to (300-350) NL / min after an aluminum block is added to the ladle, performing a melting reaction for 4.5-5.5 minutes, adding fluorite during the reaction, and then immediately adjusting the argon flow rate to (50-100) NL / min, continuing to melt for 4.5-5.5 minutes, and then increasing the argon flow rate to (200-300) NL / min until the melting reaction is completed; compared with the prior art, the invention does not affect the output of a single furnace, and effectively solves the problem of slag overflow in the ladle during aluminum alloying of 38CrMoAl steel.

The present invention belongs to the technical field of well completion engineering for unconventional oil and gas reservoirs such as petroleum and natural gas, and specifically relates to a mandrel-removable soluble bridge plug and a salvage tool used to replace the wellhead after the fracturing construction of the oil and gas well is completed, aiming to solve the problem that the existing bridge plug cannot be used for wellhead replacement operations without injecting liquid into the wellbore. The present invention provides a mandrel-removable soluble bridge plug, including a soluble bridge plug body and a mandrel arranged in the bridge plug body; the bridge plug body and the mandrel are positioned at a locking position by a limiting mechanism, and the limiting mechanism can be disengaged from the locking position under a squeezing force. The present invention is suitable for wellhead replacement operations, and there is no need to pump liquid into the well. The fracturing wellhead can be replaced with a gas production wellhead in a manner that does not pollute the reservoir and does not affect the production, thereby saving operation costs and time.

The invention discloses a non-transgenic method for producing a low-nicotine tobacco mutant plant, which comprises the following steps: infecting a tobacco plant by using an engineered RNA (Ribonucleic Acid) virus vector carrying CRISPR / Cas nuclease, and editing one or more genes of a tobacco berberine bridging enzyme-like protein gene family in a targeted manner by nuclease; carrying out in-vitro tissue culture on tobacco cells infected by viruses to regenerate tobacco mutant plants; homozygous tobacco mutant plants which are obviously reduced in nicotine content, normal in growth and development and free of influence on yield are screened. The method has the following beneficial effects that the EMDV can efficiently and stably express Cas9 protein and guide RNA in infected tobacco tissues, so that multi-copy gene editing is realized; an exogenous DNA sequence does not need to be introduced into a to-be-modified cell in the gene editing process, and the risk of exogenous gene integration does not exist; nicotine of a / b / d1 / d2 / e and a / b / e mutants is obviously reduced, and the yield is not influenced.

The invention relates to the field of compound preparation, in particular to a method for preparing thiamine hydrochloride. The method comprises the following steps: carrying out contact reaction on thiamine nitrate, ammonium chloride and hydrogen chloride under the condition of an organic solvent, and separating reaction liquid to obtain thiamine hydrochloride solid. Compared with the prior art, ammonium chloride is used for replacing part of hydrochloric acid to participate in the reaction in the thiamine hydrochloride production process, so that the dosage of hydrochloric acid is reduced, equipment corrosion can be reduced, subsequent wastewater treatment steps are simplified, the product yield is not influenced, the content of other impurities is not increased, new production equipment is not added, and the production cost is effectively saved; furthermore, the materials are recycled, so that a more environment-friendly and cost-saving process route can be realized.

The invention discloses a short-day strawberry breeding target gene sequence screening method, which comprises: S1, RNA extraction: taking leaves of short-day strawberries and four-season strawberries before and after flower bud differentiation, and extracting RNA by using a plant RNA rapid extraction kit; s2, carrying out integrity verification on the extracted RNA, detecting the purity and the concentration, and sequencing the RNA; s3, carrying out reverse transcription on the extracted RNA, designing a qRT-PCR (quantitative reverse transcription-polymerase chain reaction) primer, and carrying out PCR reaction to obtain a FaELF3 gene; and S4, cloning the FaELF3 gene in vitro and verifying the deletion mutant function recovery plasmid. The method effectively overcomes the limitation of traditional phenotype screening, fills up the market blank of the current high-temperature-resistant strawberry variety, has a wide application prospect, and is particularly suitable for the strawberry planting industry in tropical and subtropical regions. According to the method disclosed by the invention, the strawberries can also normally bloom at a relatively high temperature, and other important agronomic characters are not influenced.

The application discloses an integrated anti-freezing pressure measuring and sampling device, which comprises a pressure measuring part and a sampling part. The pressure measuring part is characterized in that an upper circular groove is formed in the upper part of a main body, the upper circular groove is connected with a union joint through an anti-freezing shell and a thin tube sleeve in an upward direction, and the union joint is connected with a pressure gauge sleeve in an upward direction. The sampling part is characterized in that a side circular groove is formed in one side of the main body and opens outward, a center hole of the side circular groove is connected with a through hole, and a sampling shell is sleeved on the inner wall of the side circular groove. A piston rod is sleeved in the axial hole of the sampling shell. An outer section of the piston rod is sleeved with a stop nut, the stop nut is sleeved with the outer section of the sampling shell, an outer end of the piston rod that extends out of the stop nut is provided with a hand wheel, and a sampling port is formed in the axial hole of the sampling shell in a downward direction. The application belongs to the technical field of petroleum and chemical equipment and solves the problem that sampling cannot be performed due to the freezing of pressure measuring and sampling ports in the prior art.

The invention relates to the technical field of corn dehydration, in particular to application of salicylhydroxamic acid in promoting corn kernel dehydration. The embodiment of the invention provides application of salicylhydroxamic acid in promoting dehydration of corn kernels for the first time. When the salicylhydroxamic acid is applied to the corn, on the premise that the yield of the corn is not affected, the grain filling and maturing process can be accelerated, the dehydration rate of the corn grains is remarkably increased, and the water content of the grains is effectively reduced.

The invention belongs to the field of biological genes, and discloses a rice monosaccharide transporter gene OsPLT1 and application of a transporter of the rice monosaccharide transporter gene OsPLT1 in regulation and control of the growth period of rice, and the nucleotide sequence of the rice monosaccharide transporter gene OsPLT1 is shown as SEQ ID NO: 1. The invention further discloses an amplification primer for the rice monosaccharide transporter gene OsPLT1. The invention provides a novel application of a rice OsPLT1 gene and an encoding protein thereof in regulating and controlling the growth period of rice. When the OsPLT1 is knocked out from the wild type ZH11 through a CRISPR / Cas9 method, the growth process of the rice can be accelerated, and the yield of the rice is not influenced, so that the OsPLT1 gene can be used for regulating the growth period of the plant and improving the annual yield of the plant, and has relatively high economic and application values.

This invention discloses a method for fermenting mung bean-based light-aroma baijiu in a gradient-temperature controlled earthen vat, comprising: S1 Raw material pretreatment, in which shelled mung beans are soaked and steamed at low temperature and then mixed with red sorghum at a mass ratio of 15-20:80-85, followed by high-temperature soaking and steaming to gelatinize; S2 Gradient-temperature controlled earthen vat fermentation, in which light-aroma low-temperature koji is added to the vat for 28 days of fermentation, with the temperature controlled at 18-22℃ in the early stage, rising to 25-28℃ and maintaining it in the middle stage, and then dropping to 15-18℃ in the later stage, with the peak temperature ≤32℃ throughout the process; S3 Distillation, in which steamed rice husks are added and the mixture is distilled, with 65% vol of the middle section of the liquor collected. This invention, through the synergistic effect of mung bean pretreatment and three-stage gradient temperature control, imparts the characteristic light aroma of mung beans while maintaining the typical style of light-aroma baijiu, resulting in a mellow, sweet, and long-lasting liquor with strong process controllability, suitable for large-scale production.

The invention belongs to the technical field of rape breeding and molecular biology, and particularly relates to a gene for assisting low-palmitic-acid rape breeding, a molecular marker and application. The invention firstly provides a gene and / or a gene composition for reducing the content of palmitic acid in rape seeds, wherein the gene composition comprises any one or more of the following genes: BnaA08. DAP, BnaA08. PAA1, BnaAO8. DUF106 and BnaC03. DAP. The invention also provides a method for preparing the gene composition for reducing the content of palmitic acid in the rape seeds, and the gene composition comprises any one or more of the following genes: BnaA08. DAP, BnaA08. PAA1, BnaAO8. DUF106 and BnaC03. DAP. Based on the gene and / or the gene composition, four low-SPAC haplotypes are identified, and a molecular marker for assisting low-palmitic acid breeding is developed at the same time. The invention is of great significance to molecular breeding of brassica napus.

The invention discloses a method for improving drought resistance and sugar content of sugarcane through genetic transformation, and belongs to the technical field of genetic engineering. By optimizing the AtDreb1A gene sequence and overexpressing the AtDreb1Asc gene in the sugarcane, the result shows that compared with a control group, the overexpressed AtDreb1Asc gene can improve the drought tolerance of the sugarcane, the sugar content is obviously higher than that of the control group, and the sugarcane can be stably inherited in the next generation. According to the invention, the transgenic sugarcane material with drought tolerance and sugar content superior to those of a control group and without influence on growth and yield is screened and cultivated, and a foundation is laid for cultivation of excellent sugarcane varieties with drought resistance, high sugar content and without influence on growth.

The utility model discloses a work cabin for magnetron sputtering target material replacement, which comprises a cabin main body, the cabin main body is of an upper opening structure, a hollow floor is arranged in the cabin main body, the hollow floor divides the cabin main body into an upper work space and a lower negative pressure space, the hollow floor is provided with a work table located in the work space, and the work table is arranged on the lower negative pressure space. A fan filter unit for communicating the outside with the negative pressure space is arranged on the cabin main body; and a cabin door for communicating the outside with the working space is arranged on the cabin main body. The dustproof workbench has the advantages that the workbench is isolated from the outside through the combination of the section bar and the transparent baffle, dust is sucked away from the position below the workbench through the fan filter unit and the hollowed-out floor, dust spreading is avoided, it is guaranteed that the cleanliness, the yield and the rework rate of a workshop are not affected, and meanwhile the risk that workers inhale ITO dust is reduced.

The invention provides a agriculture, light and wind complementary system. The agriculture, light and wind complementary system comprises a wind-light same-cable power generation string which is formed by connecting a plurality of wind driven generators and a plurality of photovoltaic cell assemblies in series and shares the same group of upper suspension cables and lower suspension cables. The photovoltaic cell assembly has a certain inclination angle and is used for guiding air flow to the wind driven generator. The wind-solar co-cable power generation strings are suspended above the ground surface such as farmland at intervals. The system does not occupy farmland and other land resources, does not interfere with agricultural machinery operation and other normal production, can relieve the contradiction of wind power land shortage, and can promote the development of agriculture, forestry and animal husbandry and clean energy.

The invention discloses a low-cadmium rice cultivation method giving consideration to grain safety production and soil cadmium extraction and remediation and application, and belongs to the technical field of genetic engineering. According to the method, specific expression of OsHMA3 in rice stems and leaves is driven by utilizing transgenic and genetic transformation technologies, so that the content of cadmium in rice is remarkably reduced. The key function of the OsHMA3 in regulation and control of cadmium accumulation of the overground part of the rice is proved for the first time, cadmium is directionally intercepted in stem and leaf tissues by inducing specific expression of the OsHMA3 in rice stems and leaves, transfer of the cadmium to grains is blocked, enrichment of cadmium in non-edible parts is promoted on the premise that absorption of soil cadmium by root systems and absorption of atmospheric sedimentation cadmium by leaf surfaces are not inhibited, and the cadmium accumulation of the overground part of the rice is promoted. The method not only ensures safe production of grains, but also realizes a mode of'simultaneous production and restoration 'for extracting cadmium from soil through straw removal, and solves the problem of how to effectively deal with atmospheric sedimentation cadmium pollution and simultaneously extract and restore cadmium from soil while reducing the cadmium content of rice.

The invention discloses a seed coating agent for improving the utilization rate of a fertilizer and a pesticide in a direct seeding rice seedling stage, a coating method and application. The coating agent adopts a framework comprising a film-forming bonding framework, a soil nutrient controlled-release component and a plant protection component. When the coated rice seeds prepared by the method are directly sown, the germination rate and the field seedling rate of the coated rice seeds are respectively increased by about 6.0% and 20.0% compared with those of uncoated rice seeds; the reduction of 10% of nitrogen application amount in any period and the reduction of 10-15% of phosphate fertilizer or 10% of potash fertilizer application amount in the whole growth period have no obvious influence on the yield. On the premise of guaranteeing the fertilizer, the reduction of 30% of pesticide has no obvious influence on the yield. Under the condition that the yield is not influenced, the base fertilizer is optimally reduced by 10% and the pesticide is optimally reduced by 20%, and the yield of the rice is not obviously different among three treatments, namely the base fertilizer is reduced by 10%, the tillering fertilizer is reduced by 10%, and the base fertilizer is reduced by 5% and the tillering fertilizer is reduced by 5%.

This invention belongs to the field of animal husbandry and deep processing of agricultural products, specifically relating to a method and its application for cultivating low-cadmium, selenium-rich mealworms using cadmium-containing feed. Specifically, this invention involves adding a low concentration of selenium to a cadmium-containing bran and rice bran feed to raise 3rd-4th instar mealworms. In the later stages of rearing, the mealworms are transferred to a selenium-only feed for cadmium removal, ensuring that the cadmium content in the mealworms does not exceed relevant standards and achieving the goal of efficiently treating cadmium-containing agricultural waste and obtaining low-cadmium, selenium-rich mealworm products. Finally, lipids in the mealworm powder are extracted in one step using low-boiling-point petroleum ether to obtain low-cadmium, selenium-rich mealworm protein powder. In summary, this invention is simple, low-cost, and can efficiently process cadmium-containing materials while simultaneously yielding low-cadmium, selenium-rich, high-quality mealworm products and mealworm protein powder, without affecting mealworm yield. It achieves multiple benefits and has good practical application value.

The present invention discloses a plugging and oil production device for a multi-point water-producing oil well and a plugging and oil production method, comprising a seating and squeezing integrated tool and a drillable pipe-inserted oil production bridge plug, a seating ring is provided below the step of the upper end convex ring of the seating and squeezing bridge plug core shaft, and a seating mechanism is provided between the seating ring and the guide head; a seating and squeezing push tube is sleeved on the outer periphery of the core shaft convex ring and abuts against the upper part of the seating and squeezing push tube, and the upper end of the seating and squeezing push tube is screwed to the outer periphery of the lower liquid cylinder piston; a tension ring is screwed into the internal thread of the core shaft convex ring, and the upper end of the tension ring is connected to the seating and squeezing lower core shaft, and the upper outer wall of the seating and squeezing lower core shaft is sealed with the inner wall of the lower liquid cylinder piston. The oil production method comprises the following steps in sequence: determining that a leaking section appears above the casing cementing section and a water-producing layer appears in the casing cementing section; completing the plugging of the leaking section above the casing cementing section; completing the plugging of the water-producing layer of the casing cementing section; carrying out oil production in the adjacent oil layer below the water-producing layer; and continuing to carry out oil production in the next oil layer. The present invention can reduce the number of times of pulling in and out the pipe string and save the operation cycle.