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Method for obtaining African daisy regeneration plant through inducing somatic embryo

A technology for somatic embryos and plant regeneration, which is applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of chlorosis and vitrification of leaves of sprouts and seedlings, and achieves high seedling rate, easy access to materials, The effect of shortening the culture period

Inactive Publication Date: 2015-06-24
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A higher concentration of 6-BA is beneficial to increase the proliferation coefficient, but it is prone to vitrification, and a high concentration of NAA will make the leaves of the sprouts turn green, so the most suitable proliferation medium is MS+6-BA 2.0mg / L+NAA 0.3mg / L

Method used

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  • Method for obtaining African daisy regeneration plant through inducing somatic embryo
  • Method for obtaining African daisy regeneration plant through inducing somatic embryo
  • Method for obtaining African daisy regeneration plant through inducing somatic embryo

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1 Obtaining Gerbera Regenerated Plants by Inducing Somatic Embryos

[0051] 1) Prepare the medium for inducing gerbera somatic embryos of the present invention, the formulation of which is shown in Table 3.

[0052] table 3

[0053]

[0054]

[0055] Among them, 35 g / L of sucrose and 6.5 g / L of agar powder were added to the induction medium, and pH=5.8-6.0.

[0056] 2) choose the gerbera young flower bud (diameter is 5-6mm, see figure 1 ), rinse with running water for 30 minutes, then stir and wash with detergent water, then disinfect with 70% (volume ratio) alcohol on the ultra-clean bench for 30-60s, and then use 0.1% (volume ratio) HgCl 2 Disinfect for 10 minutes, and finally rinse 5 times with sterile water, blot the surface moisture and set aside.

[0057] 3) On the ultra-clean bench, peel off the calyx and the small inflorescence on the small flower bud with a scalpel, and cut off its outer layer.

[0058] 4) The whole receptacle is inoculated in...

Embodiment 2

[0067] Example 2 Method for Obtaining Gerbera Regenerated Plants by Inducing Somatic Embryos

[0068] With reference to the cultivation process in Example 1, except that the content of the plant growth regulator in the induction medium in Table 1 was adjusted to the content shown in Table 5, the rest of the steps were the same as in Example 1.

[0069] table 5

[0070] Plant Growth Regulator

Dosagemg /

6-BA

7

KT

0.8

TDZ

0.05

NAA

0.4

PIC

0.3

[0071] Among them, mg / L represents the number of milligrams of each component contained in each liter of medium, sucrose in the medium is 30g / L; agar powder is 6.5, pH=5.8-6.0.

[0072] The induction rate of somatic embryos was 45.5%, and the seedling rate of small buds was 100%.

[0073] Utilizing the present invention to cultivate gerbera tissue culture seedlings can simplify the culture procedure, shorten the culture period, and improve seedling quality, and t...

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Abstract

The invention discloses a method for obtaining an African daisy regeneration plant through inducing a somatic embryo. The method comprises the steps that an African daisy receptacle serving as an explant material is cultured in a somatic embryo inducing culture medium containing 6-benayl aminopurine, kinetin, thidiazuron, naphthylacetic acid, picloram and casein hydrolysate; the somatic embryo is induced; after the somatic embryo germinates, the somatic embryo is subjected to successive transfer culture in a differential medium; and the regeneration plant is obtained. According to the method, the African daisy receptacle serves as an explant, and the regeneration plant is obtained through a somatic embryo approach without a callus stage, so that a situation that explant is mutated due to dedifferentiation inducing callus regeneration can be avoided. By utilizing the method to perform African daisy tissue culture for seedlings, the culture procedure is simplified, the culture cycle is shortened, the quality of the seedlings is improved, and base materials are provided for a large number of high-quality seedlings by in-vitro propagation of African daisy and transgenic research.

Description

technical field [0001] The invention belongs to the technical field of gerbera tissue culture, and in particular relates to a method for obtaining regenerated gerbera plants by inducing somatic embryos. Background technique [0002] Gerbera jamesonii (Gerbera jamesonii), also known as gerbera flower, is native to South Africa and is a perennial herb of the Compositae genus Gerbera. It has the advantages of rich color, unique flower shape, high flower yield and long ornamental period. one. Since Gerbera chrysanthemum is self-fertile, the seedlings have great variation, and division propagation can maintain the original species characteristics without variation, but the seedlings are easily infected by diseases and insect pests during the propagation process, limited by seasons, and the propagation speed is slow, etc. The problem is that it is difficult to adapt to the needs of large-scale and factory production, and there are still problems such as virus accumulation and spe...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 殷丽青孙翊李水根陆锦明张永春周音范晓芬
Owner SHANGHAI ACAD OF AGRI SCI
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