Immune sensor for detecting picloram in compost, and production and use method thereof

Abstract

The invention discloses an immuno-sensor for detecting picloram in compost and a manufacturing method and a using method thereof. The immuno-sensor comprises a carbon paste electrode and a picloram antibody -Fe3O4 magnetic nanoparticle crosslinking body coated on the surface of the carbon paste electrode. By utilizing the enzyme-linked immune technology and an electrochemical analysis mean, a linear regression equation of current change and the content of the picloram is established, and then the content of the picloram in the compost is rapidly and sensitively detected according to the regression equation. The method of the invention is applied to the detection of the content of the picloram in the compost and has the advantages of rapid and simple operation, rapid response, and good selectivity, etc. Furthermore, the method is a rapid picloram detection technology with low cost which can be applied to a control system of municipal solid waste compost.

Description

technical field [0001] The invention relates to an immune sensor and a preparation method thereof, and also relates to the application of the immunoelectrophoresis method in the detection and analysis of organic pollutants. Background technique [0002] Persistent organic pollutants (POPs) are special pollutants with environmental persistence, bioaccumulation, long-distance migration and high biological toxicity. They are extremely harmful to the global ecological environment and human health, and are stealthily eroding human , they have carcinogenicity, mutagenicity, teratogenicity, neurotoxicity, reproductive toxicity, endocrine disrupting properties, immunosuppressive properties, etc. In 2001, in the "Stockholm Convention on Persistent Organic Pollutants" signed in Stockholm, the capital of Sweden, the first batch of 12 types of organic pollutants included in the convention control belonged to organochlorine compounds. Organochlorine compounds are widely used organic com...

AI Technical Summary

Problems solved by technology

[0003] At present, liquid chromatography, gas chromatography and other methods are widely used for the determination of picloram content. These methods generally require the detection object to be of high purity, and the sample needs to be separated before detection. The separation process usually requires pretreatment. It is cumbersome and time-consuming; in addition, the detection equipment is expensive and inconvenient to carry, and cannot perform real-time detection

Now radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) are also used to measure picloram content, but because RIA needs the label of radioactive elements, it is easy to pollute the environment and cause harm to the health of operators; and ELISA requires multiple washing and reagent transfer processes, with many analysis steps and large errors, which is not convenient for analysis system integration, miniaturization and timely output of signals

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