Immune sensor for detecting picloram in compost, and production and use method thereof

An immune sensor and picloram technology, applied in the application field of detection and analysis, can solve the problems of environmental pollution, inconvenient analysis system integrated signal output, and inability to perform real-time detection, so as to improve sensitivity and anti-interference ability, solve real-time On-Line Monitoring Problems, Effects of Accurate Target Identification Capabilities

Inactive Publication Date: 2008-12-03
HUNAN UNIV
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Problems solved by technology

[0003] At present, liquid chromatography, gas chromatography and other methods are widely used for the determination of picloram content. These methods generally require the detection object to be of high purity, and the sample needs to be separated before detection. The separation process usually requires pretreatment. It is cumbersome and time-consuming; in addition, the detection equipment is expensive and inconvenient to carry, and cannot perform real-time detec

Method used

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  • Immune sensor for detecting picloram in compost, and production and use method thereof
  • Immune sensor for detecting picloram in compost, and production and use method thereof
  • Immune sensor for detecting picloram in compost, and production and use method thereof

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Embodiment

[0031] Utilize immunosensor and detection method of the present invention to measure 3 groups of compost leachate samples containing picloram, the detection steps and detection results are as follows:

[0032] 1. Preparation of immunosensor

[0033] like figure 1 As shown, first make a carbon paste electrode, put a carbon rod 3 in the PVC pipe 2, put a magnet 4 close to the end of the carbon rod 3 and 8mm away from the nozzle of the PVC pipe 2, and form a magnetic region, and then use the carbon paste 5 to seal the pipe The mouth of the carbon rod 3 is closed, and the other end of the carbon rod 3 is led out of the PVC pipe 2 through the wire 1 to obtain a magnetic carbon paste electrode; the surface of the electrode is polished, and then the surface of the electrode is washed with water, and then HNO 3 (mass fraction is 50%), acetone, water carry out ultrasonic cleaning, finally rinse with phosphate buffer saline, dry naturally; Then under nitrogen protection, prepare Fe 3...

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Abstract

The invention discloses an immuno-sensor for detecting picloram in compost and a manufacturing method and a using method thereof. The immuno-sensor comprises a carbon paste electrode and a picloram antibody -Fe3O4 magnetic nanoparticle crosslinking body coated on the surface of the carbon paste electrode. By utilizing the enzyme-linked immune technology and an electrochemical analysis mean, a linear regression equation of current change and the content of the picloram is established, and then the content of the picloram in the compost is rapidly and sensitively detected according to the regression equation. The method of the invention is applied to the detection of the content of the picloram in the compost and has the advantages of rapid and simple operation, rapid response, and good selectivity, etc. Furthermore, the method is a rapid picloram detection technology with low cost which can be applied to a control system of municipal solid waste compost.

Description

technical field [0001] The invention relates to an immune sensor and a preparation method thereof, and also relates to the application of the immunoelectrophoresis method in the detection and analysis of organic pollutants. Background technique [0002] Persistent organic pollutants (POPs) are special pollutants with environmental persistence, bioaccumulation, long-distance migration and high biological toxicity. They are extremely harmful to the global ecological environment and human health, and are stealthily eroding human , they have carcinogenicity, mutagenicity, teratogenicity, neurotoxicity, reproductive toxicity, endocrine disrupting properties, immunosuppressive properties, etc. In 2001, in the "Stockholm Convention on Persistent Organic Pollutants" signed in Stockholm, the capital of Sweden, the first batch of 12 types of organic pollutants included in the convention control belonged to organochlorine compounds. Organochlorine compounds are widely used organic com...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/561G01N27/327
Inventor 章毅曾光明汤琳陈立娟梁婕张长黄国和
Owner HUNAN UNIV
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