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Proliferation medium for Sierra cowberry blueberry tissue culture and preparation method thereof
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A proliferation medium and tissue culture technology, which is applied in the field of medium for the proliferation of bilberry tissue culture seedlings, can solve the problems of slow growth rate, low multiplier of philodendron, and influence on the rapid development of bilberry
Active Publication Date: 2014-10-08
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology
However, due to the use of the existing conventional tissue culture rapid propagation technology, the multiplication factor of philodendron is low and the growth rate is slow, which affects the rapid development of bilberry. It is extremely important to optimize the formula of bilberry culture medium.
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Embodiment 1
[0029] A proliferation medium for bilberry tissue culture, comprising the following components: improved WPM medium+NAA 0.5 mg / L+GA 0.2 mg / L+ZT 2mg L -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , the rest is distilled water, pH is 5.2;
[0030] Wherein the improved WPM medium comprises the following components:
[0050] A proliferation medium for bilberry tissue culture, comprising the following components: improved WPM medium+NAA 0.5 mg / L+GA 0.2 mg / L+ZT 3mg L -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , the rest is distilled water, pH is 5.2;
[0051] Wherein the improved WPM medium comprises the following components:
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technical field [0001] The invention relates to the technical field of planttissue culture and rapid propagation, and mainly relates to a medium suitable for multiplication of bilberrytissue culture seedlings. Background technique [0002] Lingonberry is a small berryfruit tree belonging to the genusVaccinium of the Rhododendron family (Ericaceae). Its fruit is blue or red, moderately sweet and sour, low in energy and contains a variety of antioxidant components, and has extremely high nutritional value and health care. The biologically active substances in the lingonberry fruit have the functions of improving eyesight, eliminating eye fatigue and enhancing eyesight. At present, scientific research institutes and enterprises all over the country have carried out a large number of studies on the tissue culture and rapid propagation of blueberry, which can quickly promote new and excellent varieties and become the main means of raising blueberry seedlings. Due to the lar...
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王姗王永平颜志明鲍华鹏许建民
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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