Proliferation medium for Sierra cowberry blueberry tissue culture and preparation method thereof

A proliferation medium and tissue culture technology, which is applied in the field of medium for the proliferation of bilberry tissue culture seedlings, can solve the problems of slow growth rate, low multiplier of philodendron, and influence on the rapid development of bilberry
CN104082142AActive Publication Date: 2014-10-08JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
Publication Date
2014-10-08
Patent Text Reader

Abstract

The invention discloses a proliferation medium for Sierra cowberry blueberry tissue culture. 1L of the proliferation medium comprises modified WPM (woody plant medium), 0.5mg of NAA (Naphthalene Acetic Acid), 0.2mg of GA, 2-3mg of zeatin (ZT), 30g of saccharose, and 7g of agar, and has pH value of 5.2. 1L of the modified WPM contains macroelements, iron salts, microelements and organic substances, wherein the macroelements comprise 893mg of potassium nitrate, 119mg of ammonium sulfate, 370mg of magnesium sulfate, 278mg of calcium nitrate tetrahydrate, and 170mg of monopotassium phosphate; the ferric salts comprise 74.6mg of ethylene diamine tetraacetic acid, and 55.6mg of ferrous sulfate; the microelements comprise 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate, 6.2mg of boric acid, 0.415mg of potassium iodide; and the organic substances comprise 0.5mg of niacin, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. The proliferation rate can reach more than 12 after the medium is used for 30 days.
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Description

technical field

[0001] The invention relates to the technical field of plant tissue culture and rapid propagation, and mainly relates to a medium suitable for multiplication of bilberry tissue culture seedlings. Background technique

[0002] Lingonberry is a small berry fruit tree belonging to the genus Vaccinium of the Rhododendron family (Ericaceae). Its fruit is blue or red, moderately sweet and sour, low in energy and contains a variety of antioxidant components, and has extremely high nutritional value and health care. The biologically active substances in the lingonberry fruit have the functions of improving eyesight, eliminating eye fatigue and enhancing eyesight. At present, scientific research institutes and enterprises all over the country have carried out a large number of studies on the tissue culture and rapid propagation of blueberry, which can quickly promote new and excellent varieties and become the main means of raising blueberry seedlings. Due to the lar...

Claims

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