Proliferation medium for Sierra cowberry blueberry tissue culture and preparation method thereof

A proliferation medium and tissue culture technology, which is applied in the field of medium for the proliferation of bilberry tissue culture seedlings, can solve the problems of slow growth rate, low multiplier of philodendron, and influence on the rapid development of bilberry

Active Publication Date: 2014-10-08
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the use of the existing conventional tissue culture rapid propagation technology, the multiplication factor of philodendron is low and the growth rate is slow, which affects the rapid development of bilberry. It is extremely important to optimize the formula of bilberry culture medium.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A proliferation medium for bilberry tissue culture, comprising the following components: improved WPM medium+NAA 0.5 mg / L+GA 0.2 mg / L+ZT 2mg L -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , the rest is distilled water, pH is 5.2;

[0030] Wherein the improved WPM medium comprises the following components:

[0031] Macroelement: 893mg·L -1 Potassium nitrate, 119 mg·L -1 Ammonium sulfate, 370 mg·L -1 Magnesium sulfate, 278 mg·L -1 Calcium nitrate, 170mg·L -1 Potassium dihydrogen phosphate;

[0032] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg·L -1 Sodium molybdate, 6.2 mg·L -1 Boric acid; 0.415 mg·L -1 Potassium iodide;

[0033] Iron salt: 74.6mg·L -1 Disodium edetate, 55.6 mg·L -1 ferrous sulfate;

[0034] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 glycine.

[0035] The above-mentioned prolif...

Embodiment 2

[0050] A proliferation medium for bilberry tissue culture, comprising the following components: improved WPM medium+NAA 0.5 mg / L+GA 0.2 mg / L+ZT 3mg L -1 + 30 g·L sucrose -1 + agar 7 g·L -1 , the rest is distilled water, pH is 5.2;

[0051] Wherein the improved WPM medium comprises the following components:

[0052] Macroelement: 893mg·L -1 Potassium nitrate, 119 mg·L -1 Ammonium sulfate, 370 mg·L -1 Magnesium sulfate, 278 mg·L -1 Calcium nitrate, 170mg·L -1 Potassium dihydrogen phosphate;

[0053] Trace elements: 22.3 mg·L -1 Manganese sulfate, 8.6 mg·L -1 Zinc sulfate, 0.025 mg·L -1 Copper sulfate, 0.25mg·L -1 Sodium molybdate, 6.2 mg·L -1 Boric acid; 0.415 mg·L -1 Potassium iodide;

[0054] Iron salt: 74.6mg·L -1 Disodium edetate, 55.6 mg·L -1 ferrous sulfate;

[0055] Organic matter: 0.5 mg·L -1 Niacin, 0.5 mg·L -1 Thiamine hydrochloride, 0.5 mg·L -1 Pyridoxine hydrochloride, l00 mg·L -1 Inositol, 2 mg·L -1 glycine.

[0056] Proliferation medium prepa...

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PUM

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Abstract

The invention discloses a proliferation medium for Sierra cowberry blueberry tissue culture. 1L of the proliferation medium comprises modified WPM (woody plant medium), 0.5mg of NAA (Naphthalene Acetic Acid), 0.2mg of GA, 2-3mg of zeatin (ZT), 30g of saccharose, and 7g of agar, and has pH value of 5.2. 1L of the modified WPM contains macroelements, iron salts, microelements and organic substances, wherein the macroelements comprise 893mg of potassium nitrate, 119mg of ammonium sulfate, 370mg of magnesium sulfate, 278mg of calcium nitrate tetrahydrate, and 170mg of monopotassium phosphate; the ferric salts comprise 74.6mg of ethylene diamine tetraacetic acid, and 55.6mg of ferrous sulfate; the microelements comprise 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate, 6.2mg of boric acid, 0.415mg of potassium iodide; and the organic substances comprise 0.5mg of niacin, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. The proliferation rate can reach more than 12 after the medium is used for 30 days.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, and mainly relates to a medium suitable for multiplication of bilberry tissue culture seedlings. Background technique [0002] Lingonberry is a small berry fruit tree belonging to the genus Vaccinium of the Rhododendron family (Ericaceae). Its fruit is blue or red, moderately sweet and sour, low in energy and contains a variety of antioxidant components, and has extremely high nutritional value and health care. The biologically active substances in the lingonberry fruit have the functions of improving eyesight, eliminating eye fatigue and enhancing eyesight. At present, scientific research institutes and enterprises all over the country have carried out a large number of studies on the tissue culture and rapid propagation of blueberry, which can quickly promote new and excellent varieties and become the main means of raising blueberry seedlings. Due to the lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王姗王永平颜志明鲍华鹏许建民
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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