Unlock instant, AI-driven research and patent intelligence for your innovation.
A kind of bilberry tissue culture proliferation medium and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A proliferation medium and tissue culture technology, which is applied in the field of medium for the proliferation of bilberry tissue culture seedlings, can solve the problems of slow growth rate, low multiplier of philodendron, and influence on the rapid development of bilberry
Active Publication Date: 2015-10-14
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
View PDF5 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, due to the use of the existing conventional tissue culture rapid propagation technology, the multiplication factor of philodendron is low and the growth rate is slow, which affects the rapid development of bilberry. It is extremely important to optimize the formula of bilberry culture medium.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0029] The improved WPM medium includes the following components:
[0030] Large elements: 893mg · L -1 Potassium nitrate, 119 mg · L -1 Ammonium sulfate, 370 mg · L -1 Magnesium sulfate, 278 mg · L -1 Calcium nitrate, 170mg · L -1 Potassium dihydrogen phosphate;
[0031] Trace element: 22.3 mg · L -1 Manganese sulfate, 8.6 mg · L -1 Zinc sulfate, 0.025 mg · L -1 Copper sulfate, 0.25mg · L -1 Sodium molybdenum, 6.2 mg · L -1 Boric acid; 0.415 mg · L -1 Potassium iodide;
[0032] Iron salt: 74.6mg · L -1 Tetharine tetharium sodium, 55.6 mg · L -1 Sulfate sulfate;
[0033] Organic material: 0.5 mg · L -1 Tobacco acid, 0.5 mg · L -1 Hydrochloride, 0.5 mg · L -1 Pharium hydrochloride, L00 mg · L -1 Musoss, 2 mg · L -1 Glycine.
[0034] The above -mentioned proliferation and cultivation system preparation methods are as follows:
[0035] (1) Preparation of improved WPM medium:
[0036] (11) Preparation of 50 times a large amount of element mother liquid: Take 44.65g of potassium nitrat...
Embodiment 2
[0049] The improved WPM medium includes the following components:
[0050] Large elements: 893mg · L -1 Potassium nitrate, 119 mg · L -1 Ammonium sulfate, 370 mg · L -1 Magnesium sulfate, 278 mg · L -1 Calcium nitrate, 170mg · L -1 Potassium dihydrogen phosphate;
[0051] Trace element: 22.3 mg · L -1 Manganese sulfate, 8.6 mg · L -1 Zinc sulfate, 0.025 mg · L -1 Copper sulfate, 0.25mg · L -1 Sodium molybdenum, 6.2 mg · L -1 Boric acid; 0.415 mg · L -1 Potassium iodide;
[0052] Iron salt: 74.6mg · L -1 Tetharine tetharium sodium, 55.6 mg · L -1 Sulfate sulfate;
[0053] Organic material: 0.5 mg · L -1 Tobacco acid, 0.5 mg · L -1 Hydrochloride, 0.5 mg · L -1 Pharium hydrochloride, L00 mg · L -1 Musoss, 2 mg · L -1 Glycine.
[0054] The method of proliferation and cultivation qualifications is as follows:
[0055] (1) Preparation of improved WPM medium:
[0056] (11) Preparation of 50 times a large amount of element mother liquid: Take 44.65g of potassium nitrate, 5.95g ammonium su...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a proliferation medium for Sierra cowberry blueberry tissue culture. 1L of the proliferation medium comprises modified WPM (woody plant medium), 0.5mg of NAA (Naphthalene Acetic Acid), 0.2mg of GA, 2-3mg of zeatin (ZT), 30g of saccharose, and 7g of agar, and has pH value of 5.2. 1L of the modified WPM contains macroelements, iron salts, microelements and organic substances, wherein the macroelements comprise 893mg of potassium nitrate, 119mg of ammonium sulfate, 370mg of magnesium sulfate, 278mg of calcium nitrate tetrahydrate, and 170mg of monopotassium phosphate; the ferric salts comprise 74.6mg of ethylene diamine tetraacetic acid, and 55.6mg of ferrous sulfate; the microelements comprise 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate, 6.2mg of boric acid, 0.415mg of potassium iodide; and the organic substances comprise 0.5mg of niacin, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. The proliferation rate can reach more than 12 after the medium is used for 30 days.
Description
Technical field [0001] The present invention involves the field of fast complexity in plant groups, mainly a medium that is suitable for the proliferation of Xili Yueyan Group. Background technique [0002] Yue Orange is the Ericaceae family.Its fruits are blue or red, moderate sweet and sour, low -energy fruits and containing a variety of antioxidant ingredients, with high nutritional value and medical care effects.The biological active substances in the orange fruit have the functions of eyesight, eliminating eye fatigue, and enhancing vision.At present, scientific research units and enterprises across the country have carried out a large number of research on the cultivation of the blue drug groups, and it can quickly promote the new and excellent varieties to become the main method of breeding of blueberry seedlings.Because there are many varieties of blueberries, the applicable proliferation medium is not the same. At present, many factory production uses a unified medium fo...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 王姗王永平颜志明鲍华鹏许建民
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
Login to View More 
Login to View More 
