A kind of bilberry tissue culture proliferation medium and preparation method thereof

A proliferation medium and tissue culture technology, which is applied in the field of medium for the proliferation of bilberry tissue culture seedlings, can solve the problems of slow growth rate, low multiplier of philodendron, and influence on the rapid development of bilberry

Active Publication Date: 2015-10-14
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the use of the existing conventional tissue culture rapid propagation technology, the multiplication factor of philodendron is low and the growth rate is slow, which affects the rapid development of bilberry. It is extremely important to optimize the formula of bilberry culture medium.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The improved WPM medium includes the following components:

[0030] Large elements: 893mg · L -1 Potassium nitrate, 119 mg · L -1 Ammonium sulfate, 370 mg · L -1 Magnesium sulfate, 278 mg · L -1 Calcium nitrate, 170mg · L -1 Potassium dihydrogen phosphate;

[0031] Trace element: 22.3 mg · L -1 Manganese sulfate, 8.6 mg · L -1 Zinc sulfate, 0.025 mg · L -1 Copper sulfate, 0.25mg · L -1 Sodium molybdenum, 6.2 mg · L -1 Boric acid; 0.415 mg · L -1 Potassium iodide;

[0032] Iron salt: 74.6mg · L -1 Tetharine tetharium sodium, 55.6 mg · L -1 Sulfate sulfate;

[0033] Organic material: 0.5 mg · L -1 Tobacco acid, 0.5 mg · L -1 Hydrochloride, 0.5 mg · L -1 Pharium hydrochloride, L00 mg · L -1 Musoss, 2 mg · L -1 Glycine.

[0034] The above -mentioned proliferation and cultivation system preparation methods are as follows:

[0035] (1) Preparation of improved WPM medium:

[0036] (11) Preparation of 50 times a large amount of element mother liquid: Take 44.65g of potassium nitrat...

Embodiment 2

[0049] The improved WPM medium includes the following components:

[0050] Large elements: 893mg · L -1 Potassium nitrate, 119 mg · L -1 Ammonium sulfate, 370 mg · L -1 Magnesium sulfate, 278 mg · L -1 Calcium nitrate, 170mg · L -1 Potassium dihydrogen phosphate;

[0051] Trace element: 22.3 mg · L -1 Manganese sulfate, 8.6 mg · L -1 Zinc sulfate, 0.025 mg · L -1 Copper sulfate, 0.25mg · L -1 Sodium molybdenum, 6.2 mg · L -1 Boric acid; 0.415 mg · L -1 Potassium iodide;

[0052] Iron salt: 74.6mg · L -1 Tetharine tetharium sodium, 55.6 mg · L -1 Sulfate sulfate;

[0053] Organic material: 0.5 mg · L -1 Tobacco acid, 0.5 mg · L -1 Hydrochloride, 0.5 mg · L -1 Pharium hydrochloride, L00 mg · L -1 Musoss, 2 mg · L -1 Glycine.

[0054] The method of proliferation and cultivation qualifications is as follows:

[0055] (1) Preparation of improved WPM medium:

[0056] (11) Preparation of 50 times a large amount of element mother liquid: Take 44.65g of potassium nitrate, 5.95g ammonium su...

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PUM

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Abstract

The invention discloses a proliferation medium for Sierra cowberry blueberry tissue culture. 1L of the proliferation medium comprises modified WPM (woody plant medium), 0.5mg of NAA (Naphthalene Acetic Acid), 0.2mg of GA, 2-3mg of zeatin (ZT), 30g of saccharose, and 7g of agar, and has pH value of 5.2. 1L of the modified WPM contains macroelements, iron salts, microelements and organic substances, wherein the macroelements comprise 893mg of potassium nitrate, 119mg of ammonium sulfate, 370mg of magnesium sulfate, 278mg of calcium nitrate tetrahydrate, and 170mg of monopotassium phosphate; the ferric salts comprise 74.6mg of ethylene diamine tetraacetic acid, and 55.6mg of ferrous sulfate; the microelements comprise 22.3mg of manganese sulfate, 8.6mg of zinc sulfate, 0.025mg of copper sulfate, 0.25mg of sodium molybdate, 6.2mg of boric acid, 0.415mg of potassium iodide; and the organic substances comprise 0.5mg of niacin, 0.5mg of thiamine hydrochloride, 0.5mg of pyridoxine hydrochloride, 100mg of inositol and 2mg of glycine. The proliferation rate can reach more than 12 after the medium is used for 30 days.

Description

Technical field [0001] The present invention involves the field of fast complexity in plant groups, mainly a medium that is suitable for the proliferation of Xili Yueyan Group. Background technique [0002] Yue Orange is the Ericaceae family.Its fruits are blue or red, moderate sweet and sour, low -energy fruits and containing a variety of antioxidant ingredients, with high nutritional value and medical care effects.The biological active substances in the orange fruit have the functions of eyesight, eliminating eye fatigue, and enhancing vision.At present, scientific research units and enterprises across the country have carried out a large number of research on the cultivation of the blue drug groups, and it can quickly promote the new and excellent varieties to become the main method of breeding of blueberry seedlings.Because there are many varieties of blueberries, the applicable proliferation medium is not the same. At present, many factory production uses a unified medium fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 王姗王永平颜志明鲍华鹏许建民
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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