3results about How to "Reduce nitrate nitrogen content" patented technology

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The invention belongs to the field of soil pollution treatment, and relates to a method for jointly relieving toxicity of microplastics to a soil-plant system based on a nitrification inhibitor-carbon nanomaterial. The method comprises the following steps: applying a nitrification inhibitor and a carbon nanomaterial to plant planting soil; the nitrification inhibitor is DMPP, and the carbon nanomaterial is one or more of a multi-walled carbon nanotube and a nanodiamond. The method can technically slow down microplastic pollution, improve soil health and guarantee quality safety of agricultural products.

This invention relates to a self-protective secondary salinization soil remediation microbial agent, its preparation method, and its application. The agent comprises the following raw materials by weight percentage: 5%–15% *Bacillus megaterium* fermentation broth, 30%–45% nitrate-containing industrial wastewater, 30%–40% organic carbon source, and the balance being water; wherein the nitrate concentration in the nitrate-containing industrial wastewater is greater than 30%; and the effective viable bacteria count is ≥200 million / mL. The preparation method involves adding the organic carbon source to the treated nitrate-containing industrial wastewater in a specific ratio and mixing thoroughly. Then, *Bacillus megaterium* fermentation broth is added in a specific ratio, and water is added to bring the total to 100%. After mixing thoroughly, the mixture is allowed to stand for at least 12 hours. For application, the soil remediation microbial agent is first prepared by fertilization, then diluted and applied as a base fertilizer or top dressing to the secondary salinization soil requiring remediation. The soil remediation microbial agent of this invention is in liquid form and can be stored for up to 3 years in a high-salt environment, exhibiting good self-protection and salt tolerance.