The invention discloses a pathological specimen processing method and belongs to the field of specimen processing technology. According to the processing method, sampled pathological tissues successively undergo fixation, dehydration, transparency, wax impregnation, embedding, slicing, staining and mounting so as to obtain a pathological specimen. The fixation is to fix the pathological tissues by the use of a fixative for 4-15 h. The fixative is composed of atractylol, ethoxylated acetylenediol, chlorogenic acid, polyvinylpyrrolidone, sodium chloride, ethyl acetate and pure water. The mounting is to carry out mounting on the stained pathological tissues by the use of a mixture of polyacrylamide, polyvinylpyrrolidone and xylene. According to the invention, toxicity of reagents used in the invention is obviously reduced, the fixation effect is good, degradation and transparency degree are easy to control. Then, the tissues are not easy to deform, embrittle or harden, and mounting is complete and staining is remarkable.