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Pathological specimen processing method

A treatment method and pathological technology, applied in the field of specimen treatment, can solve problems such as long time, no effective improvement in safety, and impact on treatment efficiency, and achieve extensive antibacterial effects, avoid sensitization effects, and strengthen the effects of fixation

Inactive Publication Date: 2017-06-30
LUOHE MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The processing method of the above specimens still uses formaldehyde for immediate fixation, and the time is long, which affects the processing efficiency, and the safety has not been effectively improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~ Embodiment 5

[0023] Embodiment 1~Example 5: Take the pathological tissue taken from human liver as an example

[0024] A method for processing pathological specimens, which is to sequentially fix, dehydrate, transparent, soak in wax, embed, section, stain and seal the pathological tissues obtained to obtain the pathological specimens, wherein:

[0025] Fixing: the pathological tissue was fixed with a fixative solution for 6 hours, and the composition of the fixative solution is shown in Table 1;

[0026]Dehydration: Use 50% ethanol, 60% ethanol, 75% ethanol and absolute ethanol as dehydrating agents in order of volume concentration, and gradually replace the water in the fixed pathological tissue. The dehydration treatment time of the above concentrations is 1~ 3 hours;

[0027] Transparency: The dehydrated pathological tissue is sequentially immersed in 50% xylene and 100% xylene solution for transparency treatment, wherein the 50% xylene solution transparency treatment time is 2 to 4 ho...

Embodiment 6~ Embodiment 11

[0035] Embodiment 6~Example 11: Take the lung pathological tissue taken from the human body as an example

[0036] A method for processing pathological specimens, which is to sequentially fix, dehydrate, transparent, soak in wax, embed, section, stain and seal the pathological tissues obtained to obtain the pathological specimens, wherein:

[0037] Fixation: Fix the pathological tissue with a fixative solution for 8 hours. The fixative solution is composed of 13g of atractyl alcohol, 7ml of ethoxylated acetylene glycol, 13g of chlorogenic acid, 40g of polyvinylpyrrolidone, and sodium chloride. 32g, ethyl acetate 6ml, pure water to 1000ml;

[0038] Dehydration: Use 50% ethanol, 60% ethanol, 75% ethanol and absolute ethanol as dehydrating agents in order of volume concentration, and gradually replace the water in the fixed pathological tissue. The dehydration treatment time of the above concentrations is kept for 2~ 4 hours;

[0039] Transparency: The dehydrated pathological t...

Embodiment 12~ Embodiment 17

[0047] Embodiment 12~Example 17: Taking the brain pathological tissue taken from the human body as an example

[0048] A method for processing pathological specimens, which is to sequentially fix, dehydrate, transparent, soak in wax, embed, section, stain and seal the pathological tissues obtained to obtain the pathological specimens, wherein:

[0049] Fixation: Fix the pathological tissue with a fixative solution for 10 hours. The fixative solution is composed of 13g of atractyl alcohol, 7ml of ethoxylated acetylene glycol, 13g of chlorogenic acid, 40g of polyvinylpyrrolidone, and sodium chloride. 32g, ethyl acetate 6ml, pure water to 1000ml;

[0050] Dehydration: Use 50% ethanol, 60% ethanol, 75% ethanol and absolute ethanol as dehydrating agents in order of volume concentration, gradually replace the water in the fixed pathological tissue, and keep the dehydration treatment time of the above concentrations for 3 hours ;

[0051] Transparency: The dehydrated pathological t...

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Abstract

The invention discloses a pathological specimen processing method and belongs to the field of specimen processing technology. According to the processing method, sampled pathological tissues successively undergo fixation, dehydration, transparency, wax impregnation, embedding, slicing, staining and mounting so as to obtain a pathological specimen. The fixation is to fix the pathological tissues by the use of a fixative for 4-15 h. The fixative is composed of atractylol, ethoxylated acetylenediol, chlorogenic acid, polyvinylpyrrolidone, sodium chloride, ethyl acetate and pure water. The mounting is to carry out mounting on the stained pathological tissues by the use of a mixture of polyacrylamide, polyvinylpyrrolidone and xylene. According to the invention, toxicity of reagents used in the invention is obviously reduced, the fixation effect is good, degradation and transparency degree are easy to control. Then, the tissues are not easy to deform, embrittle or harden, and mounting is complete and staining is remarkable.

Description

technical field [0001] The invention belongs to the technical field of specimen processing, and in particular relates to a method for processing pathological specimens. Background technique [0002] Biopsy pathological diagnosis is the first diagnosis in surgery and is the gold standard. Doctors can make a correct diagnosis based on the test results of the specimens and formulate the next treatment plan for the disease. The accuracy of pathology reports plays a decisive role in the diagnosis and treatment of diseases to a considerable extent. Correct handling of pathological specimens can not only provide an effective guarantee for pathological diagnosis and clinical diagnosis, but also maintain the patient's body and mind, and prevent disputes caused by improper specimen handling. Existing surveys have shown that the current specimen handling methods in the operating room cannot effectively prevent nursing errors. [0003] The most critical steps in the processing of pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 申现锋苏艳
Owner LUOHE MEDICAL COLLEGE
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