The invention relates to the field of foods and beverages, and discloses podocarpus macrophyllus fruit wine and a preparation method thereof. The podocarpus macrophyllus fruit wine is prepared from two fresh fruits of podocarpus macrophyllus fruits and pawpaw and five medicinal and edible traditional Chinese medicines of hawthorns, codonopsis pilosula, small red beans, agastache rugosus and jujubes. The preparation method comprises the following steps: mashing two fresh fruits, performing enzymolysis to obtain self-flowing juice, crushing the hawthorns, the small red beans and the jujubes, performing steaming, preparing liquid medicines from the codonopsis pilosula and the agastache rugosus, mixing the self-flowing juice, the steamed products and the liquid medicines, and performing alcoholic fermentation, distilling, sealing and aging, and blending to obtain the fruit wine. The podocarpus macrophyllus fruit wine opens up a new application of the podocarpus macrophyllus fruits, the fruit wine prepared by the method does not contain any additive, no toxic substance is generated in the preparation process, the operation is simple, and the obtained fruit wine is delicious in taste, bright in color, strong in fruity flavor, rich in nutrition and unique in flavor. The podocarpus macrophyllus fruit wine has the health-care effects of promoting the production of body fluid to relievethirst, tonifying middle-Jiao, benefitting Qi, clearing away heat and toxic materials, maintaining beauty, keeping young, delaying senescence, relieving fatigue, helping sleep and the like, and has wide market application prospects.