Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea

A cultivation method and a technique for regenerating buds, which are applied in the field of plant biology and can solve problems such as poor growth of regenerated buds of Echinacea purpurea

Active Publication Date: 2014-01-01
广州市泰丰源实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a kind of medium that promotes the growth of echinacea regenerated buds in order to overcome the defects of poor growth of echinacea regenerated buds in the prior art

Method used

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  • Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea
  • Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea
  • Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea

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Experimental program
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Effect test

Embodiment 1

[0025] S1. Preparation of regenerated buds: first obtain the petiole explant of Echinacea purpurea, the petiole explant comes from the diploid aseptic seedling of Echinacea purpurea under tissue culture conditions (the preparation of the explant can refer to the conventional technical method in the art), and then the petiole The explants were inoculated on regeneration medium (regeneration medium containing 30 g / L sucrose, 0.3 mg / L BA, 0.01 mg / L NAA, solidified with 4.5 g / L agar, in 1.4 kg cm -2 Sterilized MS medium for 20 min). When the regenerated shoot grows to about 2 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated shoot.

[0026] S2. Cultivation of regenerated shoots: cut out the shoots obtained from the diploid Echinacea purpurea petiole explants in step S1, and inoculate them in the culture medium supplemented with different concentrations of DA-6 (0, 0.08, 0.16, 0.32 mg / L) ...

Embodiment 2

[0032] S1. Preparation of regenerated buds: first obtain the petiole explants of Echinacea purpurea, and the petiole explants are from triploid aseptic seedlings of Echinacea purpurea under tissue culture conditions (the preparation of explants can refer to the conventional technical methods in the art), and then the petiole The explants were inoculated on the regeneration medium (the regeneration medium contained 30 g / L sucrose, 0.3 mg / L BA, 0.01 mg / L NAA, solidified with 4.5 g / L agar, in 1.4 kg cm -2 Sterilized MS medium for 20 min). When the regenerated shoot grows to about 2 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated shoot.

[0033] S2. Cultivation of regenerated shoots: cut out the shoots obtained from the petiole explants of triploid Echinacea purpurea described in step S1, and inoculate them with different concentrations of DA-6 (0, 0.08, 0.16, 0.32 mg / L) On th...

Embodiment 3

[0038] S1. Preparation of regenerated buds: firstly obtain the petiole explants of Echinacea purpurea, the petiole explants are from the tetraploid aseptic seedlings of Echinacea purpurea under tissue culture conditions (the preparation of explants can refer to the conventional technical method in the art), and then the petiole The explants were inoculated on regeneration medium (regeneration medium containing 30 g / L sucrose, 0.3 mg / L BA, 0.01 mg / L NAA, solidified with 4.5 g / L agar, in 1.4 kg cm -2 Sterilized MS medium for 20 min). When the regenerated shoot grows to about 2 cm high with leaves, the bud is cut off from the explant tissue, which can be used as the material used in the present invention—the regenerated shoot.

[0039] S2. Cultivation of regenerated shoots: cut out the shoots obtained from the petiole explants of tetraploid Echinacea purpurea described in step S1, and inoculate them into the plants supplemented with different concentrations of DA-6 (0, 0.08, ...

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Abstract

The invention belongs to the field of plant biotechnologies and particularly discloses a culture medium and culture method for promoting the growth of regeneration buds of echinacea purpurea. According to the culture medium, DA-6 (diethyl aminoethyl hexanoate) of corresponding concentration is particularly added to an MS formula in accordance with that the regeneration buds of different ploidies have different sensitivities to DA-6, besides adding 15-60g/L of saccharose, 3-9g/L of agar and 0.01-0.2mg/L of NAA (naphthalene acetic acid). The culture medium has the advantage that the height, weight, the weight, diameter and number of roots, the length of primary roots, total root length, root cap ratio and transplanting survival rate of the regeneration buds all can be increased remarkably. By applying the culture medium and the culture method, the development of the scientific research work related to the biotechnological breeding of echinacea purpurea can be promoted.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a culture medium and a culture method for promoting the growth of regenerated buds of Echinacea purpurea. Background technique [0002] Echinacea ( Echinacea purpurea L.) is a perennial herb of Compositae, which has a good immune regulation effect, and its extract is an immune enhancer and immune regulator that has received widespread attention in the world in recent years. Echinacea has become a world-renowned medicinal plant due to its precise efficacy. In order to obtain multiple varieties of Echinacea purpurea, people usually use tissue culture technology for regeneration or regenerative cloning. Regeneration or regenerative cloning of plants using tissue culture techniques usually involves two steps. The first step is to induce adventitious shoots from explants. The second step is to grow the regenerated shoots into complete plants ready for transplanting. For Echina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈晓鹭杨跃生吴鸿李栋梁
Owner 广州市泰丰源实业有限公司
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