Rooting induction method of test-tube plantlet of cunninghamia lanceolata

A technology for test-tube seedlings and Chinese fir, which is applied in the field of rooting induction of fir test-tube seedlings, can solve the problems of shortening the rooting time of Chinese fir, non-uniform rooting of Chinese fir seedlings, and a small number of roots, so as to facilitate root germination, fast rooting speed, and strong root system Effect

Active Publication Date: 2013-08-07
GUANGXI FORESTRY RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After adopting this method to carry out Chinese fir rooting culture, can shorten the rooting time of Chinese fir, improve the rooting rate of Chinese fir, but still can't solve the problem that Chinese fir seedlings are not uniform in rooting, the number of roots is few and the root system is not strong

Method used

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  • Rooting induction method of test-tube plantlet of cunninghamia lanceolata
  • Rooting induction method of test-tube plantlet of cunninghamia lanceolata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for inducing rooting of test-tube shoots of Chinese fir. Cut 1-2 cm seedlings for proliferation. A small mass of callus is retained when the seedlings are cut. The seedlings are inoculated in 1 / 2MS as the basic medium, and NAA 0.02mg / L, 2.5% sucrose and 0.7% agar in a pre-rooting medium. Cultivate in an environment with a temperature of 23±2℃, light intensity of 2200-2400LX, light time of 13h and humidity of 40-45%.

[0026] After 15 days of pre-rooting culture, the seedlings were transferred to NH 4 NO 3 The content is 660 mg / L, FeSO 4 ·7H 2 The modified 1 / 2 MS basic medium with O content of 27.8 mg / L also includes rooting medium composed of IBA 0.10 mg / L, IAA 0.05 mg / L, sucrose 2.5% and agar 0.7%. Cultivate in an environment with a temperature of 23±2℃, light intensity of 2200-2400LX, light time of 13h and humidity of 40-45%. The rooting rate of the test-tube plantlets was 88.9%, the average number of roots / strip was 4.98, and the adventitious root forming abi...

Embodiment 2

[0028] A method for inducing rooting of test-tube seedlings of Chinese fir. Cut 1-2 cm seedlings for proliferation and sub-generation. When cutting the seedlings, keep a small mass of callus, and inoculate the seedlings in 1 / 2MS as the basic medium, which also includes NAA 0.06mg / L, 2.5% sucrose and 0.7% agar in a pre-rooting medium. Cultivate in an environment with a temperature of 23±2℃, light intensity of 2200-2400LX, light time of 13h and humidity of 40-45%.

[0029] After 20 days of pre-rooting culture, the seedlings were transferred to NH 4 NO 3 The content is 660 mg / L, FeSO 4 ·7H 2 The modified 1 / 2 MS basic medium with an O content of 27.8 mg / L also includes rooting medium composed of IBA 0.15 mg / L, IAA 0.05 mg / L, sucrose 2.5% and agar 0.7% agar. Cultivate in an environment with a temperature of 23±2℃, light intensity of 2200-2400LX, light time of 13h and humidity of 40-45%. The rooting rate of the test-tube plantlets was 90.1%, the average number of roots / strip was 5.02...

Embodiment 3

[0031] A method for inducing rooting of test-tube shoots of Chinese fir. Cut 1-2 cm seedlings for proliferation and sub-generation. When cutting the seedlings, keep a small mass of callus, and inoculate the small mass of callus in 1 / 2MS as the basic medium, including NAA 0.08mg / L, sucrose 2.5% and agar 0.7% pre-rooting medium. Cultivate in an environment with a temperature of 23±2℃, light intensity of 2200-2400LX, light time of 13h and humidity of 40-45%.

[0032] After 20 days of pre-rooting culture, the seedlings were transferred to NH 4 NO 3 The content is 660 mg / L, FeSO 4 ·7H 2 The modified 1 / 2 MS basic medium with an O content of 30.9 mg / L also includes rooting medium composed of IBA 0.20 mg / L, IAA 0.06 mg / L, sucrose 2.5% and agar 0.7%. Cultivate in an environment with a temperature of 23±2℃, light intensity of 2200-2400 LX, light time of 13h and humidity of 40-45%. The rooting rate of test-tube seedlings was 93.1%, the average number of roots / strip was 5.89, and the advent...

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Abstract

The invention discloses a rooting induction method of a test-tube plantlet of cunninghamia lanceolata. The rooting induction method comprises the steps of: cutting a 1-2cm multiplication subculture plantlet, inoculating in a pre-rooting culture medium for culturing, wherein the pre-rooting culture medium uses 1/2MS-MS as a base culture medium, and also comprises 0.02-0.08mg/L of NAA (Naphthalene Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; carrying out rooting culture after pre-rooting and culturing for 15-25 days, wherein a rooting culture medium uses improved 1/2 MS as a basic culture medium, and also comprises 0.10-0.25mg/L, 0.05-0.08mg/L of IAA (Indole-3-Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; and placing in a proper environment for culturing to obtain a rooted test-tube plantlet. After the cunninghamia lanceolata is rooted and cultured by adopting the rooting induction method, the rooting rate, the root system quantity and the root system germination uniformity of the test-tube plantlet of the cunninghamia lanceolata can be increased, more excellent rooted test-tube plantlets are provided for production and application, and good economic benefit and social benefit are obtained.

Description

Technical field [0001] The invention relates to asexual reproduction technology, in particular to a method for inducing rooting of test tube seedlings of Chinese fir. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) Is an evergreen tree of the Chinese fir family. It has a history of more than a thousand years of cultivation in my country and plays an important role in the development of forestry. It can be called the "king of trees" in southern China. Cunninghamia lanceolata is the main afforestation tree species in southern my country. It has a large cultivation area and a wide range of uses. It is favored by the masses. Therefore, vigorously developing Chinese fir seedling technology to meet the market's demand for high-quality seedlings is one of the important issues currently facing. Tissue culture technology is a kind of rapid asexual reproduction technology. The use of tissue culture to breed Chinese fir seedlings is an important means to accelerate the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄开勇郝海坤陈琴蓝肖唐庆兰戴俊张照远
Owner GUANGXI FORESTRY RES INST
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