Rooting induction method of test-tube plantlet of cunninghamia lanceolata

A technology for test-tube seedlings and Chinese fir, which is applied in the field of rooting induction of fir test-tube seedlings, can solve the problems of shortening the rooting time of Chinese fir, non-uniform rooting of Chinese fir seedlings, and a small number of roots, so as to facilitate root germination, fast rooting speed, and strong root system Effect
CN103229723AActive Publication Date: 2013-08-07GUANGXI FORESTRY RES INST
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI FORESTRY RES INST
Publication Date
2013-08-07

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Abstract

The invention discloses a rooting induction method of a test-tube plantlet of cunninghamia lanceolata. The rooting induction method comprises the steps of: cutting a 1-2cm multiplication subculture plantlet, inoculating in a pre-rooting culture medium for culturing, wherein the pre-rooting culture medium uses 1 / 2MS-MS as a base culture medium, and also comprises 0.02-0.08mg / L of NAA (Naphthalene Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; carrying out rooting culture after pre-rooting and culturing for 15-25 days, wherein a rooting culture medium uses improved 1 / 2 MS as a basic culture medium, and also comprises 0.10-0.25mg / L, 0.05-0.08mg / L of IAA (Indole-3-Acetic Acid), 2.5 percent of cane sugar and 0.7 percent of agar; and placing in a proper environment for culturing to obtain a rooted test-tube plantlet. After the cunninghamia lanceolata is rooted and cultured by adopting the rooting induction method, the rooting rate, the root system quantity and the root system germination uniformity of the test-tube plantlet of the cunninghamia lanceolata can be increased, more excellent rooted test-tube plantlets are provided for production and application, and good economic benefit and social benefit are obtained.
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Description

Technical field

[0001] The invention relates to asexual reproduction technology, in particular to a method for inducing rooting of test tube seedlings of Chinese fir. Background technique

[0002] Chinese fir( Cunninghamia lanceolata ) Is an evergreen tree of the Chinese fir family. It has a history of more than a thousand years of cultivation in my country and plays an important role in the development of forestry. It can be called the "king of trees" in southern China. Cunninghamia lanceolata is the main afforestation tree species in southern my country. It has a large cultivation area and a wide range of uses. It is favored by the masses. Therefore, vigorously developing Chinese fir seedling technology to meet the market's demand for high-quality seedlings is one of the important issues currently facing. Tissue culture technology is a kind of rapid asexual reproduction technology. The use of tissue culture to breed Chinese fir seedlings is an important means to accelerate the ...

Claims

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