Rooting induction method for tissue-cultured seedlings of Lithocarpus hancei (Benth.) Rehd

A technique for tissue cultured seedlings and Keke keel, applied in the field of induction of rooting of kebab tissue cultured seedlings, can solve problems such as uneven rooting, not thick root system, and small number of roots, so as to achieve more roots, shorten the rooting cycle, and develop roots fast effect

Inactive Publication Date: 2017-01-04
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the subcultured buds of Coryne sylvestris that have been cultured for 35-36 days in the conventional tissue culture. After the surface of the bottle is sterilized, in the aseptic space on the ultra-clean workbench, select the single bud cluster that grows robustly and has a height of 1-2 cm. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+vitamin C6g / L++VC 15mg / L+glucose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for rooting cult...

Embodiment 2

[0026] Select the subcultured buds of Keke keel that have been cultured for 36-37 days in conventional tissue culture. After disinfecting the surface of the bottle, in the aseptic space on the ultra-clean workbench, select a single bud cluster that grows robustly and has a height of 1-2 cm. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+vitamin C6g / L++VC 15mg / L+glucose 25g / L+agar 5.0g / L.

[0027] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for rooting culture. The raw m...

Embodiment 3

[0030] Select the subcultured buds of Coryne sylvestris that have been cultured for 37-38 days in the conventional tissue culture, and after the surface of the bottle is disinfected, in the aseptic space on the ultra-clean workbench, select a single bud cluster that grows robustly and has a height of 1-2 cm. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+vitamin C6g / L++VC 15mg / L+glucose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium, and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for rootin...

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PUM

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Abstract

The invention discloses a rooting induction method for tissue-cultured seedlings of Lithocarpus hancei (Benth.) Rehd. According to the method, the proliferation buds of Lithocarpus hancei (Benth.) Rehd, which are cultured for 35-40d from strong buds in conventional tissue culture, are selected and further pruned, and then inoculated into a pre-rooting culture medium for 30-35d pre-rooting culture before being transferred into a rooting culture medium for culture. The rooting induction method has the following advantages: the rooting period of the proliferation buds of Lithocarpus hancei (Benth.) Rehd is shortened, and a high rooting percentage, abundance of root system, good quality and a high transplanting survival rate of rooting tissue-cultured seedlings are achieved; industrialized tissue-culture seedling raising of Lithocarpus hancei (Benth.) Rehd can be realized to provide good-quality seedlings for development of an artificial clonal forest; and the method has better economical, social and ecological benefits.

Description

technical field [0001] The invention relates to the technique of asexual propagation of Fructus fruticosa, in particular to a method for inducing rooting of Fructus fragrans tissue cultured seedlings. Background technique [0002] hard shell Ke ( Lithocarpus hancei (Benth.) Rehd ) also known as Chipigang (Taiwan), Jiuyan (Jiangxi), Bangzhang (Hunan), Shishanzhao (Guangxi), belongs to Fagaceae (Fagaceae) Lithocarpus (Lithocarpus), tree, rarely more than 15 meters high, All parts glabrous except inflorescence rachis and cupule gray pubescent. Branchlets yellowish gray or gray, often with a thin transparent waxy layer. Leaves are papery to hard leathery, ovate, obovate, broadly elliptic, obovate-elliptic, narrowly oblong-elliptic or lanceolate. The flowering period is from April to June, and the fruit matures from September to December of the following year. Bark dark gray or brownish gray, 6-10 mm thick, irregularly lobed longitudinally, peeled in small flakes, inner bark ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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