Rootage induction method of lithocarpus amygdalifolius tissue culture seedlings

A kind of technology of Xingyeke group and Xingyeke, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of small number of roots, not thick roots, uneven rooting, etc., and achieve fast rooting speed , shorten the rooting cycle, the effect of more roots

Inactive Publication Date: 2017-02-22
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the secondary buds of Apricot yeke that have been cultured for 35-36 days in conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select a single shoot that grows robustly and has a height of 1-2 cm in the secondary bud cluster. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+activated carbon 6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d...

Embodiment 2

[0026] Select the secondary buds of Apricot yeke that have been cultured for 36-37 days in the conventional tissue culture. After the surface of the bottle is disinfected, in the aseptic space on the ultra-clean workbench, select a single shoot that grows robustly and has a height of 1-2 cm in the secondary bud cluster. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+activated carbon 6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0027] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and lig...

Embodiment 3

[0030] Select the secondary buds of Apricot foliaceae that have been cultured for 37-38 days in conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select a single shoot that grows robustly and has a height of 1-2 cm in the secondary bud cluster. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+activated carbon 6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light ...

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PUM

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Abstract

The invention discloses a rootage induction method of lithocarpus amygdalifolius tissue culture seedlings. The method comprises the steps of selecting lithocarpus amygdalifolius subculture buds from strong buds cultured for 35-40d in conventional tissue culture, inoculating to a pre-rootage culture medium after further selecting and trimming, and transferring to a rootage culture medium for culture after pre-rootage culture for 30-35d. The method shortens a rootage cycle of the lithocarpus amygdalifolius subculture buds; the subculture buds have a high rootage rate, many root systems and good quality; the rootage tissue culture seedlings have a high transplanting survival rate; and the method can achieve industrial seedling raising of lithocarpus amygdalifolius tissue culture, provides the high quality seedlings for an artificial clonal forest and has better economic benefits, social benefits and ecologic benefits.

Description

technical field [0001] The invention relates to the asexual propagation technology of Xingyeke, in particular to a method for inducing rooting of tissue cultured seedlings of Xingyeke. Background technique [0002] Apricot leaves ( Lithocarpus amygdalifolius (Skan) Hayata ), also known as Lithocarpus apricot leaf, red peony, and Lingmei, is an arbor of the genus Lithocarpus in the family Fagaceae. The new branches and young leaves extracted in spring and summer are densely covered with yellow-brown curly pilose, and all the hairs fall off after autumn . Male spikes axillary in single spike or multiple spikes arranged in panicles, rachis densely pilose; female flowers in clusters of 3, sometimes scattered single. The cupule is nearly spherical, full of nuts, in a discontinuous ring; the wall of the nut is slightly thicker than that of the cupola, and the top of the nut is puberulent. Distributed in central and southern Taiwan, southern Fujian, southeastern Guangdong about ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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