A method for promoting the rooting of Eucalyptus durn tissue culture seedlings

A tissue culture seedling and tissue culture technology, applied in the field of plant reproduction, can solve the problems such as the difficulty of rooting in Eucalyptus Dunn tissue culture, and achieve the effects of obvious advancement, vigorous vitality, and improved rooting rate and transplanting survival rate.

Inactive Publication Date: 2018-01-05
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Dunn Eucalyptus tissue culture rooting is more difficult

Method used

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  • A method for promoting the rooting of Eucalyptus durn tissue culture seedlings
  • A method for promoting the rooting of Eucalyptus durn tissue culture seedlings
  • A method for promoting the rooting of Eucalyptus durn tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The 8-year-old Dunn's Eucalyptus tree selected and bred in Hechi, Guangxi was used as the mother plant, and the young shoots of the year were collected for grafting on the stock. After the grafting survived, it was moved to the nursery of the Institute of Biology, Guangxi Academy of Forestry for management. According to the national industry standard for the production of Eucalyptus tissue cultured seedlings, routine inoculation, disinfection and subculture were carried out, and the subcultured buds of 15 times / 30 days of subculture were used as experimental materials to carry out the rooting treatment of Eucalyptus dunen tissue cultured seedlings.

[0028] Transfer the Eucalyptus dunen tissue-cultured shoots with an average height of 2.0-3.0cm to medium I, at a temperature of 25±1°C, with a light source of 650nm red light:450nm blue light=1.5:1 red and blue LED lights, and a light intensity of 1500 -2000lx, under the light and temperature conditions of light time 16h / d,...

Embodiment 2

[0034] The 8-year-old Dunn's Eucalyptus tree selected and bred in Hechi, Guangxi was used as the mother plant, and the young shoots of the year were collected for grafting on the stock. After the grafting survived, it was moved to the nursery of the Institute of Biology, Guangxi Academy of Forestry for management. According to the national industry standard for the production of Eucalyptus tissue cultured seedlings, routine inoculation, disinfection and subculture were carried out, and the subcultured buds of 20 subcultures / 30 days of subculture were used as experimental materials to carry out the rooting treatment of Eucalyptus dunen tissue cultured seedlings.

[0035] Transfer the Eucalyptus dunen tissue-cultured shoots with an average height of 2.0-3.0cm to medium I, at a temperature of 25±1°C, with a light source of 650nm red light:450nm blue light=1.5:1 red and blue LED lights, and a light intensity of 1500 -2000lx, under the light and temperature conditions of light time ...

Embodiment 3

[0041] The 8-year-old Dunn's Eucalyptus tree selected and bred in Hechi, Guangxi was used as the mother plant, and the young shoots of the year were collected for grafting on the stock. After the grafting survived, it was moved to the nursery of the Institute of Biology, Guangxi Academy of Forestry for management. According to the national industry standard for the production of Eucalyptus tissue cultured seedlings, routine inoculation, disinfection and subculture were carried out, and the subcultured buds of 30 times / subculture cycle 30 days were used as experimental materials to carry out the rooting treatment of Eucalyptus dunen tissue cultured seedlings.

[0042] Transfer the Eucalyptus dunen tissue-cultured shoots with an average height of 2.0-3.0cm to medium I, at a temperature of 25±1°C, with a light source of 650nm red light:450nm blue light=1.5:1 red and blue LED lights, and a light intensity of 1500 -2000lx, under the light and temperature conditions of light time 16h...

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Abstract

The invention provides a method for promoting the rooting of Eucalyptus dunnii Maiden tissue culture seedlings. The method comprises the steps of robust seedling cultivation, adventitious root induction, adventitious root elongation and seedling transplanting, wherein Eucalyptus dunnii Maiden tissue culture successive-generation cluster buds are transferred into a culture medium I for the robust seedling cultivation; then, the obtained successive-generation buds are sequentially inoculated in a culture medium II and a culture medium III to obtain rooted regenerated plants by using a two-step rooting method; finally, the rooted regenerated plants are transplanted into a seedling medium to obtain the rooted Eucalyptus dunnii Maiden tissue culture seedlings. According to the invention, based on a mechanism that Eucalyptus dunnii Maiden is difficult to root, root-promoting polyphenols are added to the culture media, so that the culture media are more scientific, easier to promote the rooting of Eucalyptus dunnii Maiden, and significant in effect; the Eucalyptus dunnii Maiden tissue culture seedlings obtained by the method have a rooting rate up to 93.2% and a transplanting survival rate of 94.0%, have significant advancement, and have good economic, social and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to tissue culture seedling raising technology, in particular to a method for promoting the rooting of Eucalyptus dunen tissue culture seedlings. Background technique [0002] In the early 1950s, my country began to build large-scale eucalyptus plantations. In the early 1980s, a large number of eucalyptus species were introduced, improved and cultivated in southern my country. The research on eucalyptus in the past 30 years has made eucalyptus the fastest growing forest resource in southern my country and the best economic benefit, and has become a development tree species generally accepted by enterprises and forest farmers. Guangxi is the main eucalyptus plantation area in my country, but the vast majority of eucalyptus plantations are concentrated in southern Guangxi. This shows that the development of eucalyptus plantations in the north and south of Guangxi is extremely...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姚瑞玲王胤
Owner GUANGXI FORESTRY RES INST
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