Lithocarpus elmerrillii tissue culture seedling rooting induction method

A Wanningke group, pre-rooting technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of not strong root system, inconsistent rooting, small number of root systems, etc., to shorten the rooting cycle and root system. The effect of many, fast rooting speed

Inactive Publication Date: 2017-02-01
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select subcultured buds of Wanningke that have been cultured for 35 to 36 days in conventional tissue culture, and after disinfecting the surface of the bottle, select single buds that grow robustly and have a height of 1 to 2 cm in the aseptic space on the ultra-clean workbench. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+vitamin C6g / L++VC 15mg / L+lactose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for rooting culture. The raw mater...

Embodiment 2

[0026] Select subcultured buds of Wanningke that have been cultured for 36 to 37 days in conventional tissue culture, and after disinfecting the surface of the bottle, select single buds that grow robustly and have a height of 1 to 2 cm in the aseptic space on the ultra-clean workbench. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+vitamin C6g / L++VC 15mg / L+lactose 25g / L+agar 5.0g / L.

[0027]After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for rooting culture. The raw materi...

Embodiment 3

[0030] Select the subcultured buds of Wanningke that have been cultured for 37-38 days in the conventional tissue culture. After disinfecting the surface of the bottle, in the aseptic space on the ultra-clean workbench, select a single bud cluster that grows robustly and has a height of 1-2 cm. For buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+vitamin C6g / L++VC 15mg / L+lactose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium, and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for rooting culture. Th...

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Abstract

The invention discloses a lithocarpus elmerrillii tissue culture seedling rooting induction method. The method comprises the steps that lithocarpus elmerrillii subculture buds subjected to bud strengthening culture in conventional tissue culture for 35 d to 40 d are selected and then further selected and pruned, a pre-rooting culture medium is inoculated with the pruned lithocarpus elmerrillii subculture buds, and after pre-rooting culture is conducted for 30 d to 35 d, the buds are transferred into a rooting culture medium to be cultured. According to the method, the rooting cycle of the lithocarpus elmerrillii subculture buds is shortened, the rooting rate is high, more root systems are generated, the quality is good, the transplanting survival rate of rooted tissue culture seedlings is high, lithocarpus elmerrillii tissue culture industrialization seedling raising can be achieved, high-quality seedlings are provided for construction of man-made clonal forests, and the good economic benefit, social benefit and ecological benefit are achieved.

Description

technical field [0001] The invention relates to the asexual reproduction technique of Wanningke, in particular to a method for inducing rooting of Wanningke tissue cultured seedlings. Background technique [0002] Wanningke ( Lithocarpus elmerrillii ), is a tree of the family Fagaceae (Lithocarpus), up to 25 meters, with longitudinal grooves on the branches in the current year, glabrous, dark brown after drying, gray-white lenticels scattered on the 2 or 3-year-old branches, and semicircular bud scales Shaped or broad triangular, oily and shiny after drying. Leaf thin leathery, long elliptic or rarely obovate elliptic, 10-17cm long, 3-6cm wide, apex acuminate, base attenuate long, extending down to base along petiole, entire, lateral Veins 9-12 on each side, sharply curved upward near the leaf margin and submerged, branch veins are slender, with a dense wax scale layer on the back of the leaf, grayish white after drying; petiole 2-2.5 cm long. Female flowers are solitary ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD
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