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Method for castanea henryi tissue culture seedling rooting in bottle

A technology for tissue culture seedlings and chestnuts, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of not thick roots, small number of roots, and uneven rooting, so as to shorten the rooting cycle and achieve root growth. The effect of fast speed and many roots

Inactive Publication Date: 2017-06-06
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the secondary buds of Castanea henryi that have been cultured with strong buds for 35-40 days in conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select single buds that grow robustly and have a height of 1 to 2 cm in the secondary bud cluster. , Cut at 2~3mm below the node to remove the base leaves and petioles. The pruned single buds were inoculated into the pre-rooting medium. The raw material content of the pre-rooting medium was: 1 / 2 modified ER medium + NAA 1.0 mg / L + vitamin C 6g / L++VC 2.0 mg / L+sugar 15g / L+agar 5.0g / L, in the environment of temperature 20±1℃, humidity 55~60%, light intensity 2000lux, light 16h / d for pre-rooting culture. After the single bud has undergone 30-35d pre-rooting culture, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 modified ER medium+IAA 1.0mg / L+ABT6#0.5 mg / L+sucrose 25g / L+agar 5.0g / L, root...

Embodiment 2

[0025] Select the secondary buds of Castanea henryi that have been cultured with strong buds for 35-40 days in conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select single buds that grow robustly and have a height of 1 to 2 cm in the secondary bud cluster. , Cut at 2~3mm below the node to remove the base leaves and petioles. The pruned single shoots were inoculated into the pre-rooting medium. The raw material content of the pre-rooting medium was: 1 / 2 modified ER medium + NAA 1.5 mg / L + vitamin C 6 g / L + VC 1.0 mg / L+sugar 15g / L+agar 5.0g / L, in the environment of temperature 20±1℃, humidity 60-65%, light intensity 2000lux, light 15h / d for pre-rooting culture. After the single bud has undergone 30-35d pre-rooting culture, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 modified ER medium+IAA 1.5mg / L+ABT6#1.0 mg / L+sucrose 25g / L+agar 5.0g / L, ...

Embodiment 3

[0028] Select the secondary buds of Castanea henryi that have been cultured with strong buds for 35-40 days in conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select single buds that grow robustly and have a height of 1 to 2 cm in the secondary bud cluster. , Cut at 2~3mm below the node to remove the base leaves and petioles. The pruned single buds were inoculated into the pre-rooting medium. The raw material content of the pre-rooting medium was: 1 / 2 modified ER medium + NAA 2.0 mg / L + vitamin C 6g / L + VC 1.5 mg / L+sugar 15g / L+agar 5.0g / L, in the environment of temperature 20±1℃, humidity 65~70%, light intensity 2500lux, light 15h / d for pre-rooting culture. After the single bud has undergone 30-35d pre-rooting culture, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 modified ER medium+IAA 2.0mg / L+ABT6#2.0 mg / L+sucrose 25g / L+agar 5.0g / L, roo...

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Abstract

The invention discloses a method for castanea henryi tissue culture seedling rooting in a bottle. According to the method, castanea henryi subculture buds subjected to bud strengthening culture in conventional tissue culture for 35 to 40d are selected; after disinfection and trimming, pre-rooting culture is firstly performed; after pre-rooting culture for 30 to 35d, rooting culture is performed; the buds are cultured in environment with the temperature being 20+ / -1 DEG C, the humidity being 55 to 70 percent, the illumination intensity being 2000 to 2500lux and the illumination being 15 to 16h / d; tissue culture seedlings with roots are obtained. The method has the advantages that the castanea henryi subculture bud rooting period is shortened; the rooting rate is high; the root systems are many; the quality is good; the transplanting survival rate of the rooting issue culture seedling is high; the castanea henryi tissue culture industrialized seedling culture can be realized; high-quality seedlings can be provided for the manual clonal forest building; better economic benefits, social benefits and ecological benefits are realized.

Description

Technical field [0001] The invention belongs to the asexual propagation technology of Castanea henryi, and relates to a method for rooting in a tissue culture seedling bottle of Castanea henryi. Background technique [0002] Castanea henryi (Skam) Rehd. et Wils., Castanea henryi (Skam) Rehd. et Wils., alias: Castanea henryi, sharp chestnut, arrow chestnut, rotundus, chestnut, etc., deciduous tree, up to 30 meters, diameter at breast height up to 1 meter . The leaves are alternate, ovate-lanceolate, 8-17 cm long and 2-5 cm wide, with a pointed tip, round base, and serrated leaf margins. Male inflorescences have lower leaf axils of branchlets, and female inflorescences have upper leaf axils of branchlets. The cupule is spherical, with a thorny diameter of 2-3.5 cm; the nut is solitary in a cupule and is ovoid. Flowering period from May to July, fruit period from September to October. It is widely distributed in the south of the southern slope of the Qinling Mountains and north ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳
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