Lithocarpus carolinae (Skan) Rehd tissue cultured seedling rooting induction method

Abstract

The invention discloses a Lithocarpus carolinae (Skan) Rehd tissue cultured seedling rooting induction method. According to the Lithocarpus carolinae (Skan) Rehd tissue cultured seedling rooting induction method, Lithocarpus carolinae (Skan) Rehd subcultured buds obtained via 35 to 40d of culturing of strong buds obtained in conventional tissue culturing are selected; further selection is carried out, clipping is carried out, a pre-rotting culture medium is inoculated with the obtained Lithocarpus carolinae (Skan) Rehd subcultured buds for 30 to 35d of pre-rooting culturing, and then the Lithocarpus carolinae (Skan) Rehd subcultured buds are delivered into a rooting culture medium for culturing. The Lithocarpus carolinae (Skan) Rehd tissue cultured seedling rooting induction method is capable of shortening rooting period of Lithocarpus carolinae (Skan) Rehd subcultured buds; rooting efficiency is high; the obtained root systems are well developed; quality is high; transplanting survival rate of rooting tissue cultured seedlings is high; industrialized seedling of Lithocarpus carolinae (Skan) Rehd tissue culturing can be realized; high quality seedlings are provided for construction of artifical clonal forests; and excellent economic, social, and ecological benefits are achieved.

Description

technical field

[0001] The invention relates to the asexual reproduction technology of Akesia chinensis, in particular to a method for inducing rooting of tissue-cultured seedlings of Akesia chinensis. Background technique

[0002] Heart Ke ( Lithocarpus carolinae (Skan) Rehd ), is a tree of the family Fagaceae (Fagaceae) of the genus Lithocarpus, about 20 meters high, 40 cm in diameter at breast height, and the top bud scales are triangular and long-lanceolate. Raw lenticels, branches, bud scales, and leaves glabrous. Leaves thick papery, oblong, sparsely obovate-oblong, 13-18 cm long, 4-6 cm wide, apex caudate shortly pointed, base wedge-pointed, midrib slightly raised on leaf surface, or upper segment flat , 15-20 on each side of the lateral veins, reaching to the tooth end of the leaf margin, or sharply curved upward near the leaf margin, gradually slender and submerged, with stellate trichomes in the vein axils, the lower part of the leaf margin is entire, and the upp...

Examples

Embodiment 1

[0022] Select the secondary buds of Hongxinke that have been cultured for 35-36 days in the conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the sterile space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for rooting culture. The raw material co...

Embodiment 2

[0026] Select the secondary buds of Hongxinke that have been cultured for 36-37 days in conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the sterile space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0027]After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for rooting culture. The raw material content...

Embodiment 3

[0030] Select the secondary buds of Hongxinke that have been cultured for 37-38 days in conventional tissue culture. After disinfecting the surface of the bottle, select a single bud that grows robustly and is 1-2 cm in height in the sterile space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium, and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for rooting culture. The raw material content of...

technical field

[0001] The invention relates to the asexual reproduction technology of Akesia chinensis, in particular to a method for inducing rooting of tissue-cultured seedlings of Akesia chinensis. Background technique

[0002] Heart Ke ( Lithocarpus carolinae (Skan) Rehd ), is a tree of the family Fagaceae (Fagaceae) of the genus Lithocarpus, about 20 meters high, 40 cm in diameter at breast height, and the top bud scales are triangular and long-lanceolate. Raw lenticels, branches, bud scales, and leaves glabrous. Leaves thick papery, oblong, sparsely obovate-oblong, 13-18 cm long, 4-6 cm wide, apex caudate shortly pointed, base wedge-pointed, midrib slightly raised on leaf surface, or upper segment flat , 15-20 on each side of the lateral veins, reaching to the tooth end of the leaf margin, or sharply curved upward near the leaf margin, gradually slender and submerged, with stellate trichomes in the vein axils, the lower part of the leaf margin is entire, and the upp...

Examples

Embodiment 1

[0022] Select the secondary buds of Hongxinke that have been cultured for 35-36 days in the conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the sterile space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for rooting culture. The raw material co...

Embodiment 2

[0026] Select the secondary buds of Hongxinke that have been cultured for 36-37 days in conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the sterile space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0027]After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for rooting culture. The raw material content...

Embodiment 3

[0030] Select the secondary buds of Hongxinke that have been cultured for 37-38 days in conventional tissue culture. After disinfecting the surface of the bottle, select a single bud that grows robustly and is 1-2 cm in height in the sterile space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+vitamin C6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium, and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for rooting culture. The raw material content of...