Rooting induction method of test-tube plantlet of cunninghamia lanceolata

A test-tube seedling and Chinese fir technology, which is applied in the field of fir test-tube seedling rooting induction, can solve the problems of shortening the rooting time of Chinese fir, non-uniform rooting of Chinese fir seedlings, and a small number of root systems, and achieves the advantages of being conducive to root germination, fast rooting, and strong root system. Effect
CN103229723BActive Publication Date: 2014-07-30GUANGXI FORESTRY RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGXI FORESTRY RES INST
Publication Date
2014-07-30

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Abstract

The invention discloses a method for inducing rooting of Chinese fir test-tube seedlings. Firstly, 1-2 cm proliferating seedlings are cut and inoculated in a pre-rooting medium for cultivation. The pre-rooting medium takes 1 / 2 MS-MS as the basic medium, and also includes NAA0.02-0.08mg / L, sucrose 2.5% and agar 0.7%. After 15-25 days of pre-rooting culture, rooting culture is carried out again. The rooting medium is based on the improved 1 / 2MS medium, including IBA0.10-0.25mg / L and IAA0.05-0.08mg / L, sucrose 2.5 % and agar 0.7%, and then cultured in a suitable environment to obtain rooted test-tube plantlets. After the Chinese fir rooting culture is carried out by this method, the rooting rate, root quantity and root germination uniformity of Chinese fir test-tube seedlings can be improved, and more high-quality rooted test-tube seedlings can be provided for production and application, which has good economic and social benefits.
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Description

technical field

[0001] The invention relates to asexual reproduction technology, in particular to a method for inducing rooting of Chinese fir test-tube seedlings. Background technique

[0002] Chinese fir (Cunninghamialanceolata) is an evergreen tree of the family Firaceae. It has been cultivated in my country for more than a thousand years and plays an important role in forestry development. It can be called the "king of trees" in southern China. As the main afforestation tree species in southern my country, fir has a large cultivation area and is widely used, and is favored by the masses. Therefore, it is one of the important problems to vigorously develop fir seedling raising technology to meet the market's demand for high-quality seedlings. Tissue culture technology is a kind of rapid asexual reproduction technology. The use of tissue culture to breed Chinese fir seedlings is an important means to accelerate the popularization and use of Chinese fir varieties and an eff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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