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Rooting induction method of test-tube plantlet of cunninghamia lanceolata

A test-tube seedling and Chinese fir technology, which is applied in the field of fir test-tube seedling rooting induction, can solve the problems of shortening the rooting time of Chinese fir, non-uniform rooting of Chinese fir seedlings, and a small number of root systems, and achieves the advantages of being conducive to root germination, fast rooting, and strong root system. Effect

Active Publication Date: 2014-07-30
GUANGXI FORESTRY RES INST
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

After adopting this method to carry out Chinese fir rooting culture, can shorten the rooting time of Chinese fir, improve the rooting rate of Chinese fir, but still can't solve the problem that Chinese fir seedlings are not uniform in rooting, the number of roots is few and the root system is not strong

Method used

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  • Rooting induction method of test-tube plantlet of cunninghamia lanceolata
  • Rooting induction method of test-tube plantlet of cunninghamia lanceolata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for inducing rooting of Chinese fir test-tube seedlings. Cut out 1-2cm proliferating and subcultured seedlings, keep a small group of callus tissue when cutting the seedlings, and inoculate the seedlings on 1 / 2MS as the basic medium, and also include NAA0.02mg / L , sucrose 2.5% and agar 0.7% in pre-rooting medium. Place them in an environment with a temperature of 23±2°C, a light intensity of 2200-2400LX, a light time of 13h / d, and a humidity of 40-45%.

[0026] After 15 days of pre-rooting culture, the seedlings were transferred to NH 4 NO 3 The content is 660mg / L, FeSO 4 ·7H 2 The modified 1 / 2MS basal medium with O content of 27.8mg / L also included rooting medium composed of IBA0.10mg / L, IAA0.05mg / L, sucrose 2.5% and agar 0.7%. Place them in an environment with a temperature of 23±2°C, a light intensity of 2200-2400LX, a light time of 13h / d, and a humidity of 40-45%. The rooting rate of the test-tube seedlings was 88.9%, the average root number / plant was ...

Embodiment 2

[0028] A method for inducing rooting of Chinese fir test-tube seedlings, cutting out 1-2cm proliferating seedlings, retaining a small group of callus tissue when cutting out the seedlings, inoculating the seedlings on 1 / 2MS as the basic medium, and including NAA0.06mg / L , sucrose 2.5% and agar 0.7% in pre-rooting medium. Place them in an environment with a temperature of 23±2°C, a light intensity of 2200-2400LX, a light time of 13h / d, and a humidity of 40-45%.

[0029] After 20 days of pre-rooting culture, the seedlings were transferred to NH 4 NO 3 The content is 660mg / L, FeSO 4 ·7H 2 The modified 1 / 2 MS basic medium with O content of 27.8mg / L also includes rooting medium composed of IBA0.15mg / L, IAA0.05mg / L, sucrose 2.5% and agar 0.7% agar. Place them in an environment with a temperature of 23±2°C, a light intensity of 2200-2400LX, a light time of 13h / d, and a humidity of 40-45%. The rooting rate of test-tube seedlings was 90.1%, the average root number / plant was 5.02, ...

Embodiment 3

[0031] A method for inducing rooting of Chinese fir test-tube seedlings, cutting out 1-2cm proliferating seedlings, retaining a small group of callus tissue when cutting the seedlings, inoculating the small group of callus tissue on 1 / 2MS as the basic medium, and including NAA0 .08mg / L, sucrose 2.5% and agar 0.7% in pre-rooting medium. Place them in an environment with a temperature of 23±2°C, a light intensity of 2200-2400LX, a light time of 13h / d, and a humidity of 40-45%.

[0032] After 20 days of pre-rooting culture, the seedlings were transferred to NH 4 NO 3 The content is 660mg / L, FeSO 4 ·7H 2 The modified 1 / 2 MS basic medium with O content of 30.9mg / L also includes rooting medium composed of IBA0.20mg / L, IAA0.06mg / L, sucrose 2.5% and agar 0.7%. Place them in an environment with a temperature of 23±2°C, a light intensity of 2200-2400LX, a light time of 13h / d, and a humidity of 40-45%. The rooting rate of test-tube seedlings was 93.1%, the average root number / plant ...

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Abstract

The invention discloses a method for inducing rooting of Chinese fir test-tube seedlings. Firstly, 1-2 cm proliferating seedlings are cut and inoculated in a pre-rooting medium for cultivation. The pre-rooting medium takes 1 / 2 MS-MS as the basic medium, and also includes NAA0.02-0.08mg / L, sucrose 2.5% and agar 0.7%. After 15-25 days of pre-rooting culture, rooting culture is carried out again. The rooting medium is based on the improved 1 / 2MS medium, including IBA0.10-0.25mg / L and IAA0.05-0.08mg / L, sucrose 2.5 % and agar 0.7%, and then cultured in a suitable environment to obtain rooted test-tube plantlets. After the Chinese fir rooting culture is carried out by this method, the rooting rate, root quantity and root germination uniformity of Chinese fir test-tube seedlings can be improved, and more high-quality rooted test-tube seedlings can be provided for production and application, which has good economic and social benefits.

Description

technical field [0001] The invention relates to asexual reproduction technology, in particular to a method for inducing rooting of Chinese fir test-tube seedlings. Background technique [0002] Chinese fir (Cunninghamialanceolata) is an evergreen tree of the family Firaceae. It has been cultivated in my country for more than a thousand years and plays an important role in forestry development. It can be called the "king of trees" in southern China. As the main afforestation tree species in southern my country, fir has a large cultivation area and is widely used, and is favored by the masses. Therefore, it is one of the important problems to vigorously develop fir seedling raising technology to meet the market's demand for high-quality seedlings. Tissue culture technology is a kind of rapid asexual reproduction technology. The use of tissue culture to breed Chinese fir seedlings is an important means to accelerate the popularization and use of Chinese fir varieties and an eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄开勇郝海坤陈琴蓝肖唐庆兰戴俊张照远
Owner GUANGXI FORESTRY RES INST
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