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Rooting method of tung oil tree tissue culture seedling in bottle

A technology for tissue culture seedlings and tung tree, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of small number of roots, uneven rooting, and not strong root system, etc., and achieve fast rooting speed and strong root system. More, the effect of shortening the rooting cycle

Inactive Publication Date: 2017-05-31
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the secondary buds of the tung tree that have been cultured for 35-40 days in the conventional tissue culture, and after the surface of the bottle is disinfected, in the aseptic space on the ultra-clean workbench, select the vigorous growth of the secondary buds and the height of 1-2cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and the raw material content of the pre-rooting medium was: 1 / 2 improved ER medium+IBA 1.0mg / L+vitamin C 6g / L++VC 2.0mg / L + carrageenan 25g / L + agar 5.0g / L, carry out pre-rooting culture in an environment with a temperature of 20±1°C, a humidity of 55-60%, a light intensity of 2000lux, and a light of 16h / d. After the single bud has been pre-rooted for 30-35 days, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 improved ER medium + IAA 1.0mg / L + ABT1#0.5 mg / L + sucrose 25g / L...

Embodiment 2

[0025] Select the secondary buds of the tung tree that have been cultured for 35-40 days in the conventional tissue culture, and after the surface of the bottle is disinfected, in the aseptic space on the ultra-clean workbench, select the vigorous growth of the secondary buds and the height of 1-2cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. Inoculate the pruned single buds in the pre-rooting medium. The raw material content of the pre-rooting medium is: 1 / 2 improved ER medium+IBA 1.5mg / L+vitamin C 6g / L++VC 1.0mg / L + carrageenan 25g / L + agar 5.0g / L, carry out pre-rooting culture in an environment with a temperature of 20±1°C, a humidity of 60-65%, a light intensity of 2000lux, and a light of 15h / d. After the single bud has been pre-rooted for 30-35 days, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 improved ER medium + IAA 1.5mg / L + ABT1#1.0 mg / L+sucrose 25g / L+agar 5.0g / L,...

Embodiment 3

[0028] Select the secondary buds of the tung tree that have been cultured for 35-40 days in the conventional tissue culture, and after the surface of the bottle is disinfected, in the aseptic space on the ultra-clean workbench, select the vigorous growth of the secondary buds and the height of 1-2cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium. The raw material content of the pre-rooting medium was: 1 / 2 improved ER medium+IBA 2.0mg / L+vitamin C 6g / L++VC 1.5mg / L + carrageenan 25g / L + agar 5.0g / L, carry out pre-rooting culture in an environment with a temperature of 20±1°C, a humidity of 65-70%, a light intensity of 2500lux, and a light of 15h / d. After the single bud has undergone pre-rooting culture for 30-35 days, the single bud is transferred into the rooting medium. The raw material content of the rooting medium is: 1 / 2 improved ER medium + IAA 2.0mg / L + ABT1#2.0 mg / L + s...

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Abstract

The invention discloses a rooting method of a tung oil tree tissue culture seedling in a bottle. According to the method, tung oil tree subculture buds subjected to bud strengthening culture for 35 to 40d in conventional tissue culture are selected; after disinfection and trimming, pre-rooting culture is firstly performed; after pre-rooting culture for 30 to 35d, rooting culture is performed; the buds are cultured in an environment with the temperature being 20+ / -1 DEG C, the humidity being 55 to 70 percent; the illumination intensity being 2000 to 2500lux and the illumination being 15 to 16h / d; tissue culture seedlings with roots are obtained. The method has the advantages that the rooting period of the tung oil tree subculture buds is shortened; the rooting rate is high; the root systems are more; the quality is good; the rooting tissue culture seedling transplanting survival rate is high; the tung oil tree tissue culture industrialized seedling culture can be realized; high-quality seedlings are provided for the building of artificial clonal forest; better economic benefits, social benefits and ecological benefits are realized.

Description

technical field [0001] The invention belongs to the asexual propagation technology of tung tree, and relates to a method for rooting in a bottle of tung tree tissue culture seedling. Background technique [0002] Tung tree (Vernicia fordii (Hemsl.) Airy Shaw), Euphorbiaceae Tung genus, is a deciduous tree with gray bark, thick branches, oval leaves, monoecious flowers, calyx densely covered with brown puberulent ; petals white, with light red veins, obovate, ovary densely pilose, drupe nearly globose, seed coat woody. The flowering and fruiting period is from March to September. Distributed in Shaanxi, Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Hubei, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Yunnan and other provinces and regions in China. Tung, together with camellia oleifera, walnut, and black tallow, are called the four major woody oil plants in China. Tung tree is born in the area above 1000 meters, likes warmth and avoids severe cold. A te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳
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