Method for rapidly breeding potted carnation through tissue culture

A tissue culture, carnation technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as provenance degradation, inter-variety hybridization, and flower color confusion, and achieve the effect of high work efficiency and simple procedures.

Active Publication Date: 2013-08-28
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In traditional production, potted Carnation mainly relies on lateral bud cuttings to expand propagation. During this long-term vegetative propagation process, it is easy to cause problems such as virus infection and provenance degradation, and sexual reproduction of seeds is prone to hybridization between varieties, resulting in flower color. confusion

Method used

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  • Method for rapidly breeding potted carnation through tissue culture
  • Method for rapidly breeding potted carnation through tissue culture
  • Method for rapidly breeding potted carnation through tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] [embodiment 1] a kind of method of tissue culture rapid breeding potted Carnation, it comprises the following steps:

[0023] S1: Explant explants are cut and sterilized, which includes the following sub-steps:

[0024] (1) Select the side buds of potted Carnation without disease, wash them in running water for 1 hour, and then absorb the water;

[0025] (2) Put it into the ultra-clean workbench and disinfect it with 70% alcohol for 35 seconds;

[0026] (3) Rinse 4 times with sterile water, put in 0.1% mercuric chloride to sterilize for 8 minutes;

[0027] (4) Rinse 4 times with sterile water and place on sterile paper to dry.

[0028] S2: Primary culture: cut the sterilized lateral buds into 1.5cm-diameter stems with buds, and inoculate them in the primary medium for culture, wherein the primary medium is MS+6-BA1.5mg / L+NAA 0.2mg / L + sucrose 30g / L + agar 8g / L, the culture conditions are: light intensity 2200LX, light time 15h, temperature 23°C, incub...

Embodiment 2

[0032] [embodiment 2] a kind of method of tissue culture rapid breeding potted carnation, it may further comprise the steps:

[0033] S1: Explant explants are cut and sterilized, which includes the following sub-steps:

[0034] (1) Select the side buds of potted Carnation without disease, wash them in running water for 2 hours, and then absorb the water;

[0035] (2) Put it into the ultra-clean workbench and disinfect it with 75% alcohol for 28 seconds;

[0036] (3) Rinse 5 times with sterile water, put in 0.2% mercuric chloride to sterilize for 5 minutes;

[0037] (4) Rinse 6 times with sterile water and dry on sterile paper.

[0038] S2: Primary culture: Cut the sterilized lateral buds into 2.5cm-diameter stems with buds and inoculate them in the primary medium for culture, wherein the primary medium is MS+6-BA1.5mg / L+NAA 0.2mg / L + sucrose 30g / L + agar 8g / L, the culture conditions are: light intensity 1800LX, light time 20h, temperature 25°C, incubation ti...

Embodiment 3

[0042] [embodiment 3] a kind of method of tissue culture rapid breeding potted Carnation, it comprises the following steps:

[0043] S1: Explant explants are cut and sterilized, which includes the following sub-steps:

[0044] (1) Select the side buds of potted Carnation without disease, wash them in running water for 1.5 hours, and then absorb the water;

[0045] (2) Put it into the ultra-clean workbench and disinfect it with 72.5% alcohol for 30 seconds;

[0046] (3) Rinse 5 times with sterile water, put in 0.15% mercuric chloride to sterilize for 6 minutes;

[0047] (4) Rinse 5 times with sterile water and dry on sterile paper.

[0048]S2: Primary culture: Cut the sterilized lateral buds into 2cm-diameter stems with buds, and inoculate them in the primary medium for culture, wherein the primary medium is MS+6-BA1.5mg / L+NAA 0.2mg / L L + sucrose 30g / L + agar 8g / L, the culture conditions are: light intensity 2000LX, light time 18h, temperature 24°C, incubation...

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Abstract

The invention discloses a method for rapidly breeding potted carnation through tissue culture. The method comprises the following steps of: cutting an explant and sterilizing; carrying out primary culture, wherein a primary culture medium comprises MS (Murashige and Skoog), 1.5mg/L of 6-BA (6-Benzylaminopurine), 0.2mg/L of NAA (Naphthalene Acetic Acid), 30g/L of sucrose and 8g/L of agar; carrying out subculture, wherein a subculture medium comprises MS, 0.8mg/L of 6-BA, 0.2mg/L of NAA, 30g/L of sucrose and 8g/L of agar; carrying out rooting culture, wherein a rooting culture medium comprises MS, 0.2mg/L of NAA, 0.1mg/L of IAA(Indole Acetic Acid), 30g/L of sucrose and 8g/L of agar; and washing the culture mediums away and then holding the temperature and humidity for seedling hardening and transplanting. The method is simple and easy to operate, simple in procedure and high in working efficiency, and is capable of effectively holding excellent properties of parents. According to the method, the differentiation rate of a tissue culture seedling is increased. The method has the characteristics of high multiplication speed, high rooting rate, high survival rate and low cost.

Description

technical field [0001] The invention relates to a method for rapid propagation of potted carnation by tissue culture. Background technique [0002] Carnation (Dianthus caryophyllus L), also known as carnation, is a perennial herbaceous perennial plant of the family Caryophyllaceae. Native to Northern and Southern Europe. There are about 80 species of plants in this genus. They are native plants of British Ireland, Northern Europe and Southern Europe. They like a cool, dry, sunny and well-ventilated ecological environment. Carnation has good cold resistance, but poor heat resistance. The optimum growth temperature is 14-21°C. When the temperature exceeds 27°C or is lower than 14°C, the plant grows slowly. Suitable for planting in humus-rich, well-drained calcareous soil, likes fertilizer. [0003] Carnation is one of the world's famous and precious flower varieties because of its delicate and fragrant flowers and long flowering period. It has a broad market prospect. Pott...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马建华冯爱云罗丽君晏强王小辉
Owner SICHUAN COLORLINK CO LTD
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