Preparation method of plant tissue culture medium of apple cold-resisting Russian stocks 150

Abstract

The invention discloses a preparation method of a plant tissue culture medium of apple cold-resisting Russian stocks 150. A formula of a proliferation culture medium comprises the following componentsaccording to following proportions: 0.3 to 0.5mg / L of basic culture medium MS, 6-benzylamino adenine, 0.3 to 0.5mg / L of indole-3-butyric acid, 20 to 40g / L of sucrose and 3.5 to 4.5g / L of agar powder;a formula of a rooting culture medium comprises the following components according to following proportions: 0.8 to 1.2mg / L of basic culture medium 1 / 2 MS, indole-3-butyric acid, 0.8 to 1.5mg / L of 1-naphthylacetic acid, 0.2 to 0.5mg / L of chlormequat chloride, 20 to 40g / L of 5 sucrose and 3.5 to 4.5g / L of agar powder. The formula of the proliferation culture medium can be used for greatly shortening a culture period; the formula of the rooting culture medium has a high rooting rate; after the stocks are inoculated for 13 days, the rooting rate is 90 to 93 percent; the domestication survival rate can be greatly improved so that the tissue culture seedling cost is reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for configuring a plant tissue culture medium. Background technique [0002] At present, in the society, most of the propagation methods of Russian Anvil 150 are based on tissue culture and rapid propagation. In other tissue culture rooms, due to the inappropriate formulation of the proliferation medium and rooting medium, the differentiation multiple is not high, only about 2 times. Therefore, The propagation speed is slow; the tissue culture rooted seedlings tend to grow excessively and the quality of the roots is not good, and some calluses grow from the cuts of the seedlings, and then take root from the callus, which causes the upper and lower parts of the seedlings to be blocked when transporting nutrients; Some of the roots that grow are a kind of puffed roots, which cannot give full play to the absorption capacity of the roots. These two situations gr...

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Problems solved by technology

[0003] The object of the present invention is exactly in order to solve above-mentioned problem, provides a kind of plant tissue culture medium configuration method, to solve existing culture medium formula unreasonable, bottle seedling differentiation multiple is not high, propagation speed is slow; Low survival rate, increased seedling production costs and other issues; at the same time solve the problem that apple rootstocks are needed in some cold regions