Preparation method of plant tissue culture medium of large cherry rootstock Gisela No. 7

Abstract

The invention discloses a preparation method of a plant tissue culture medium of a large cherry rootstock Gisela No. 7. A formula and proportions of a multiplication culture medium are as follows: a basic culture medium MS, 0.3 to 0.8mg / L 6-benzylaminoadenine, 0.5 to 1.0mg / L indole-3-butyric acid, 20 to 40g / L of sucrose and 3.5 to 4.5g / L agar powder; a formula and proportions of a rooting culturemedium are as follows: a basic culture medium 1 / 2MS, 0.1 to 0.5mg / L indole-3-butyric acid, 0.1 to 0.5mg / L 1-naphthylacetic acid, 20 to 40g / L sucrose and 3.5 to 4.5g / L agar powder. By adopting the formula of the multiplication culture medium, the culture period is greatly shortened, nearly no mutation is caused and produced seedlings have high uniformity; the formula of the rooting culture medium has a high rooting rate; after the rooting culture medium is used for culturing for 12 days, the rooting rate is 92 to 97 percent and the number of roots of each seedling is 4 to 10; especially, the roots directly grow from a seedling basic part and calluses are not formed between the roots and the seedling; normal roots grow and puffed roots do not grow; the domestication survival rate can be greatly improved through the two characteristics so that the cost of tissue culture seedlings is reduced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for configuring a plant tissue culture medium. Background technique [0002] At present, the propagation methods of Gisela No. 7 rootstock mainly include tissue culture rapid propagation and twig cuttings. The root system of young shoot cuttings is weak, the life span is short, and the propagation is limited by seasons. In contrast, tissue culture and rapid propagation are more suitable for the propagation of Gisela No. 7 rootstock. However, in some tissue culture rooms, due to the inappropriate formulation of the proliferation medium and rooting medium, the differentiation factor is not high, only about 2 times, so the propagation speed is slow; The callus grows first, and then takes root from the callus, so that the upper and lower parts of the seedlings cannot conduct nutrients smoothly; some roots are a kind of puffed root, which cannot fully exert the ...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is exactly in order to solve above-mentioned problem, provides a kind of plant tissue culture medium configuration method, to solve existing culture medium formula unreasonable, bottle seedling differentiation multiple is not high, propagation speed is slow; Low survival rate, increased seedling production costs and other issues; at the same time solve the problem that apple rootstocks are needed in some cold regions