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Artificial high-efficiency clonal propagation method of dendrobium anosmum

A technology of Dendrobium sandalwood and clones, applied in the field of ornamental plant reproduction, can solve the problems of difficulty in large-scale production and promotion in large areas, low rate of protocorm differentiation into seedlings, large variation of seedlings, etc., so as to improve the survival rate of seedling domestication. , the effect of shortening the breeding cycle and fast proliferation

Inactive Publication Date: 2020-11-24
云南华农农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similar to other Dendrobium plants that have been reported, it is not difficult for the seeds of Dendrobium sandalwood to germinate asynchronously with artificial assistance, but the growth is slow, the rate of protocorm differentiation into seedlings is not high, and the seedlings vary greatly, and there are large The proportion of deformed seedlings makes it difficult to carry out large-scale production, standardized (consistent genotype background) planting and large-scale promotion of high-quality seedlings

Method used

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  • Artificial high-efficiency clonal propagation method of dendrobium anosmum
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  • Artificial high-efficiency clonal propagation method of dendrobium anosmum

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Effect test

Embodiment 1

[0054] A kind of artificial high-efficiency clonal propagation method of dendrobium dendrobii comprises the following steps:

[0055] 1. Acquisition of explants: Select healthy plants with good growth potential, no damage by diseases and insect pests, and no deformities, and get the stem section under the tip of the shoot, including the second to third sections, and cut it into about 1.0cm long stem section with a scalpel.

[0056] 2. Remove the leaves from the section of the segmented stem in step 1 and wash it with running water, soak it in a washing powder solution with a mass ratio of 15% for 10 minutes, rinse it with running water for 30 minutes, and place it in an ultra-clean workbench; soak it in an ethanol solution with a volume ratio of 75% 10-15s, then sterilized with 0.1% mercuric chloride aqueous solution by mass ratio for 6 minutes, during which the bottle body was shaken constantly to achieve the best sterilization effect, and finally rinsed with sterile water for...

Embodiment 2

[0068] A kind of artificial high-efficiency clonal propagation method of dendrobium dendrobii comprises the following steps:

[0069] 1. Acquisition of explants: Select a strong plant with good growth potential, no damage by diseases and insect pests, and no deformity, and get the stem section under the tip of the shoot, including the 2nd to 4th sections, and cut it into about 1.5cm long stem section with a scalpel.

[0070] 2. Remove the leaves from the section of the segmented stem in step 1 and wash it with running water, soak it in a washing powder solution with a mass ratio of 15% for 10 minutes, rinse it with running water for 30 minutes, and place it in an ultra-clean workbench; soak it in an ethanol solution with a volume ratio of 75% 10-15s, then sterilized with 0.1% mercuric chloride aqueous solution by mass ratio for 6 minutes, during which the bottle body was shaken constantly to achieve the best sterilization effect, and finally rinsed with sterile water for 3 time...

Embodiment 3

[0081] A kind of artificial high-efficiency clonal propagation method of dendrobium dendrobii comprises the following steps:

[0082] 1. Acquisition of explants: Select robust plants with good growth potential, no damage by diseases and insect pests, and no deformities, and get the stem section under the tip of the shoot, including the 2nd to 5th sections, and cut it into about 2.0cm long stem sections with a scalpel.

[0083] 2. Remove the leaves from the section of the segmented stem in step 1 and wash it with running water, soak it in a washing powder solution with a mass ratio of 15% for 10 minutes, rinse it with running water for 30 minutes, and place it in an ultra-clean workbench; soak it in an ethanol solution with a volume ratio of 75% 10-15s, then sterilized with 0.1% mercuric chloride aqueous solution by mass ratio for 6 minutes, during which the bottle body was shaken constantly to achieve the best sterilization effect, and finally rinsed with sterile water for 3 ti...

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Abstract

The invention provides an artificial high-efficiency clonal propagation method of dendrobium anosmum. The method comprises the following steps: inoculating a dendrobium anosmum stem explant with budssubjected to disinfection sterilization treatment into a culture medium A, and performing culturing under the conditions that the illuminance is 1500-2000lx and the illumination time is 10h / d; starting multiplication culture under the condition that the temperature is controlled to be 25 + / -1 DEG C, so that the base number of the material is increased, and the multiplication coefficient can be about 2.35 after 75 days; shearing the material into a stem segment containing 2-3 nodes, inoculating the stem segment into a culture medium B, and performing culturing for 60 days to form a multi-bud clustered system with a propagation coefficient of 6.0 or above; enabling adventitious roots to be synchronously induced in the culture medium, wherein the rooting rate reaches 100%; and the survival rate of rooted seedlings reaching 100% after the rooted seedlings are subjected to acclimatization for 60 days. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, provides technical support for protecting wild resources and breeding high-quality plantlets, and can produce excellent plantlets with consistent genotype backgrounds in a short time.

Description

technical field [0001] The invention relates to the technical field of ornamental plant propagation, in particular to an artificial high-efficiency clone propagation method of dendrobium dendrobii. Background technique [0002] Dendrobium anosmum Lindl. belongs to the spring Dendrobium species, with small and bright flowers, long flowering period and a faint sandalwood fragrance. It is distributed in Vietnam, Malay Peninsula, Thailand and other Southeast Asian countries; Dendrobium is very ornamental and commercial in Dendrobium orchids It is also an important parental material for breeding, and has become a hot orchid species in Southeast Asia in recent years. [0003] Some orchid cultivation companies in Yunnan, my country introduced about 2,000 Dendrobium sandalwood from Vietnam in 2017-2018. The fruit is a capsule, the seeds are small and have no endosperm, and it is extremely difficult to germinate under natural conditions. Similar to other Dendrobium plants that have ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈建颖曾彪黄衡宇
Owner 云南华农农业有限公司
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