Artificial high-efficiency clonal propagation method of dendrobium anosmum
A technology of Dendrobium sandalwood and clones, applied in the field of ornamental plant reproduction, can solve the problems of difficulty in large-scale production and promotion in large areas, low rate of protocorm differentiation into seedlings, large variation of seedlings, etc., so as to improve the survival rate of seedling domestication. , the effect of shortening the breeding cycle and fast proliferation
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[0053] Example 1
[0054] A method for artificial and efficient clonal propagation of Dendrobium spp. includes the following steps:
[0055] 1. Obtaining explants: select robust plants with good growth vigor, no diseases and insect pests, and no deformities. Take the stems below the tip of the stem, including the 2nd to 3rd sections, and cut them into long stems of about 1.0cm with a scalpel.
[0056] 2. After removing the leaves from the stems with knots in step 1 and washing them in running water, soak them in a detergent powder solution with a mass ratio of 15% for 10 minutes, rinse them with running water for 30 minutes, and place them in an ultra-clean workbench; soak them in a 75% ethanol solution by volume 10-15s, then sterilize with a 0.1% mercury liter aqueous solution for 6 minutes, during which the bottle body is shaken continuously to achieve the best sterilization effect, and finally rinsed with sterile water 3 times, each time not less than 3 minutes, and placed on the ...
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[0067] Example 2
[0068] A method for artificial and efficient clonal propagation of Dendrobium spp. includes the following steps:
[0069] 1. Obtaining explants: select robust plants with good growth vigor, no pests and diseases, and no deformities. Take the stem section below the stem tip, including the second to the fourth section, and cut it into about 1.5cm long stem section with a scalpel.
[0070] 2. After removing the leaves from the stems with knots in step 1 and washing them in running water, soak them in a detergent powder solution with a mass ratio of 15% for 10 minutes, rinse them with running water for 30 minutes, and place them in an ultra-clean workbench; soak them in a 75% ethanol solution by volume 10-15s, then sterilize with a 0.1% mercury liter aqueous solution for 6 minutes, during which the bottle body is shaken continuously to achieve the best sterilization effect, and finally rinsed with sterile water 3 times, each time not less than 3 minutes, and placed on ...
Example Embodiment
[0080] Example 3
[0081] A method for artificial and efficient clonal propagation of Dendrobium spp. includes the following steps:
[0082] 1. Obtaining explants: select robust plants with good growth potential, no diseases and insect pests, and no deformities. Take the stems under the apex, including the 2nd to 5th nodes, and cut them into about 2.0cm long stems with a scalpel.
[0083] 2. After removing the leaves from the stems with knots in step 1 and washing them in running water, soak them in a detergent powder solution with a mass ratio of 15% for 10 minutes, rinse them with running water for 30 minutes, and place them in an ultra-clean workbench; soak them in a 75% ethanol solution by volume 10-15s, then sterilize with a 0.1% mercury liter aqueous solution for 6 minutes, during which the bottle body is shaken continuously to achieve the best sterilization effect, and finally rinsed with sterile water 3 times, each time not less than 3 minutes, and placed on the filter paper ...
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