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44results about How to "No variation" patented technology

Method for cultivating seedlings by means of green branches under full exposure and light mist condition

The invention belongs to the field of crop growing and particularly relates to a method for cultivating seedlings by means of green branches under the full exposure and light mist condition. According to the method for cultivating seedlings by means of green branches under the full exposure and light mist condition, seedling cultivation facilities are simple, investment is low, the influence on blooming, in the next year, of a female parent is small, plants which can grow under natural condition can be cultivated directly at a time, and the survival rate can reach 90%. The method for cultivating the seedlings by means of green branches under the full exposure and light mist condition comprises the following steps of (1) seedbed manufacturing, (2) branch selection and ear trimming, (3) hole forming and cuttage, (4) mist spraying control, (5) management after cuttage, and (6) seedling hardening, wherein according to mist spraying control, after cuttage, secondary sterilization is conducted, a high-pressure light mist control system is started at the same time, five billion mist particles are generated by a nozzle per second, the diameter of each mist particle ranges from 0.5 micron to 5 microns, and a water film exist on the surface of each leaf all the time under the full exposure condition; according to management after cuttage, sterilization is conducted in the day when cuttage is conducted, mist spraying is conducted every seven days since then, mist spraying is conducted every ten to fifteen days after the seedlings strike roots, mist spraying is stopped in the later stage, foliage dressing is conducted once every ten days, embryogenic calluses appear after seven to eight days generally, and a large amount of roots are generated after fourteen days.
Owner:WUHAN ZHONGXING OASIS ECOLOGICAL AGRI & FORESTRY SCI & TECH DEV CO LTD

Quick breeding method for root of kudzu vine

The quick breeding method of kudzu vine includes the following steps: cutting kudzu vine with leaf into 5-8mm long bud section, soaking the bud section in breeding liquid for 1-2 hr, sealing the end cuts of bud section with the compounded cut treating material, setting the bud section in nursery bed with spread lower perlite grain layer of 3cm thickness and upper fine sand layer of 3cm thickness and covering arched shed, growing the bud section in the shed with controlled temperature 15-33deg.c, humidity of 70 % and illumination rate of 30 % to form complete regenerated plant within 20 days. The method is simple, fast in breeding speed, and suitable for fast breeding of kudzu vine.
Owner:陈清虎

Centrifugal disc type micro-fluidic chip and application method thereof

The invention provides a centrifugal disc type micro-fluidic chip which comprises a chip body and a magnetic bead transferring module, a plurality of centrifugal units are arranged on the chip body, the centrifugal units comprise different fluid chambers, each fluid chamber comprises a reaction reagent, magnetic beads and an organic reagent, the magnetic beads can move among different fluid chambers, the organic reagent is used for reducing the viscosity and interfacial tension of the reaction reagent, so that the magnetic beads can be conveniently separated from the reaction reagent and thenmove to other fluid chambers, the magnetic bead transferring module is used for providing magnetic driving force to enable the magnetic beads to be transferred into different fluid chambers. Accordingto the invention, the viscosity and interface tension of the reaction reagent are reduced by adding the organic reagent, the problem that magnetic beads are difficult to transfer in the prior art issolved, and particularly, the problem of difficulty in transferring the micro magnetic beads is solved, and the e magnetic beads can be transferred without increasing magnetic force and the magnetic bead amount.
Owner:DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD

Simple device for expanding deep holes and cooling and chip removal method

The invention discloses a simple device for expanding deep holes and a cooling and chip removal method. The simple device comprises a machine body, a working table, a movable drilling platform and a pulsed compressed air source; the machine body is provided with a longitudinal screw pair and a feed motor; the working table is provided with a table base, a handwheel, a horizontal screw pair and a platform; the movable drilling platform is provided with a speed reducer, a drilling motor and a drilling stem, and the drilling stem is provided with an auger bit; the pulsed compressed air source is a device for supplying pulsed airflow which comprises an air pump, an electromagnetic valve, an intermittent switching control circuit, a flexible output tube and a blowing tube. The cooling and chip removal method is that during hole expansion, the pulsed airflow supplied by the pulsed compressed air source blows into a flow passage from the hole-expanding feed end of the auger bit, so that chips produced in hole expansion are blown out from the other end of the flow passage. The simple can achieve the object of reducing cost and increasing quality and efficiency for the expansion of deep holes of heat exchange plates.
Owner:宁海国基模材有限公司

Edgeworthia chrysantha Lindl. cutting breeding method and Edgeworthia chrysantha Lindl. transplant method

The invention relates to the field of seedling planting, and especially relates to an Edgeworthia chrysantha Lindl. cutting breeding method and an Edgeworthia chrysantha Lindl. transplant method. The cutting breeding method comprises preparing a cutting medium, processing cuttings, performing cutting and performing management after cutting; the process of preparing the cutting medium comprises preparing an original cutting medium and performing disinfection on the original cutting medium, wherein the original cutting medium comprises coconut shell powder, peat soil and perlite with a volume ratio of (2-3):(1-2):(1-2); the process of processing cuttings comprises processing the base parts of the cuttings by a rooting agent, wherein the rooting agent comprises at least one of naphthylacetic acid, indole acetic acid and indolebutyric acid; the process of management after cutting comprises moisture management and temperature management; and cutting seedling transplant can be performed 50-65 days after the cutting breeding, and then transplant management is performed. The cutting breeding method can ensure fast rooting of the Edgeworthia chrysantha Lindl. cuttings, and the rooted cutting seedlings have strong living ability after the transplant, so that the rooting and survival rate of the transplant are increased. Cutting breeding can keep excellent characters of female parents; no variation occurs; and thus uniformity of seedlings is kept.
Owner:HUAYUAN LANDSCAPE ARCHITECTURE CO LTD

Seedling raising method of full light green branches and micro mist

The invention belongs to the field of crop growing and particularly relates to a method for cultivating seedlings by means of green branches under the full exposure and light mist condition. According to the method for cultivating seedlings by means of green branches under the full exposure and light mist condition, seedling cultivation facilities are simple, investment is low, the influence on blooming, in the next year, of a female parent is small, plants which can grow under natural condition can be cultivated directly at a time, and the survival rate can reach 90%. The method for cultivating the seedlings by means of green branches under the full exposure and light mist condition comprises the following steps of (1) seedbed manufacturing, (2) branch selection and ear trimming, (3) hole forming and cuttage, (4) mist spraying control, (5) management after cuttage, and (6) seedling hardening, wherein according to mist spraying control, after cuttage, secondary sterilization is conducted, a high-pressure light mist control system is started at the same time, five billion mist particles are generated by a nozzle per second, the diameter of each mist particle ranges from 0.5 micron to 5 microns, and a water film exist on the surface of each leaf all the time under the full exposure condition; according to management after cuttage, sterilization is conducted in the day when cuttage is conducted, mist spraying is conducted every seven days since then, mist spraying is conducted every ten to fifteen days after the seedlings strike roots, mist spraying is stopped in the later stage, foliage dressing is conducted once every ten days, embryogenic calluses appear after seven to eight days generally, and a large amount of roots are generated after fourteen days.
Owner:WUHAN ZHONGXING OASIS ECOLOGICAL AGRI & FORESTRY SCI & TECH DEV CO LTD

Efficient seedling cultivating method for leafbuds of Yunshan 2# grape novel variety

The invention discloses an efficient seedling cultivating method for leafbuds of a Yunshan 2# grape novel variety. The method comprises the following steps: (1) automatic control of environmental factors; (2) maternal plant plantation management of Yunshan 2# grapes; (3) preparation of an in vitro material; (4) treatment of the in vitro material; (5) seedbed inoculation and management; and (6) on-site hardening-seedling and transplantation. By using semi-lignified leafbuds as the in vitro material to cultivate seedlings, the seedling cultivating method disclosed by the invention breaks through the conventional seedling cultivating technological forms such as seeds, cuttage, tissue culture and the like so as to form technical innovation of Yunshan 2# seedling cultivation. The seedling cultivating method disclosed by the invention can realize the following technical indexes: characteristics of the original variety Ziwuhua grape: high yield and bumper crop, stress resistance and the like are ensured, and no variation is available; the seedling cultivating period is shortened to 60 days, and the highest seedling cultivating number of times reaches 6 time; the seedling survival rate reaches over 95%; the cost of Yunshan 2# seedlings can be lowered to 30 yuan/strain.
Owner:QINGDAO LEYI MODERN AGRI SCI & TECH DEMONSTRATION BASE CO LTD

Establishment method of turbot hepatic parenchymal cell line and turbot hepatic parenchymal cell line

The invention relates to an establishment method of turbot hepatic parenchymal cell line and the turbot hepatic parenchymal cell line. The establishment method of the turbot hepatic parenchymal cell line comprises the following steps: obtaining turbot liver tissue; cutting the liver tissue into tissue blocks, and carrying out washing; carrying out digestion treatment by adopting type 4 collagenase, and carrying out washing again; carrying out digestion treatment on the tissue blocks obtained after the digestion treatment by adopting trypsin again; adding a primary complete medium to terminate digestion to obtain treated tissue blocks; uniformly attaching the treated tissue blocks into a culture bottle, placing the attached culture bottle into an incubator at 23 DEG C for 6 hours; inverting the culture bottle for 6 hours; adding 4 ml of a primary complete culture medium after the culture bottle is upright; performing primary culture; and then, performing subculture. The turbot hepatic parenchymal cell line established by the method can realize continuous passage so as to provide a large number of turbot hepatic cells; and the established stable and sustainable turbot hepatocyte line can be applied to the molecular cell biology research of turbot nutrition metabolism, thereby overcoming defects in the prior art at the cellular level.
Owner:OCEAN UNIV OF CHINA

Method for removing Leifsonia xyli subsp.xyli and quickly breeding healthy cane seedling

InactiveCN101361459BSalient Features and Notable ImprovementsEfficient and thorough removalHorticulture methodsPlant tissue cultureComing outDisease
The invention relates to the propagation production of cane seedlings, and provides a method for removing cane perennial root dwarfing germs and rapidly propagating healthy cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated plantlet by carrying out speckle enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after coming out from nursery, cane stem juice is extracted by sampling, and RSD virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. Themethod has the advantages that: dwarfing disease pathogenic bacteria of the perennial root of the cane seedling can be thoroughly removed, annually propagation coefficient can reach as high as more than 10 thousand times, furthermore, the seedling has no variation, has the advantages of being fast, high-efficient and safe, and is suitable for the industrialized and standardized production of the cane healthy seedlings on scale.
Owner:GUANGZHOU SUGARCANE IND RES INST

Traditional Chinese medicine for treating hepatitis

The invention discloses a traditional Chinese medicine for treating hepatitis, and the traditional Chinese medicine is prepared from the following raw materials: radix isatidis, folium isatidis, cordate houttuynia, radix rehmanniae praeparata, cornus officinalis, ginseng, radix salviae miltiorrhizae, radix astragali, honeysuckle flowers, pseudo-ginseng, poria with hostwood, platycladi seeds, radix asparagi, rhizoma ligustici wallichii, fructus schizandrae, liquorice, polygala tenuifolia, cinnabar, and the like. The traditional Chinese medicine disclosed by the invention can be used for treating both symptoms and root causes, has the effects of diminishing inflammation and resisting viruses, nourishing liver, nourishing yin and clearing heat, tonifying Qi and replenishing blood, nourishing heart and calming nerves, eliminating evil and strengthening healthy energy, is proved to have obvious effects of inhibiting and clearing hepatitis viruses, regulating the immunologic functions of a human body, restoring liver form and function and preventing and treating liver fibrosis and canceration, has the characteristics of low treatment cost, less dosage, no toxic or side effect and fast enzyme reduction on bilirubin abnormity, glutamic-pyruvic transaminase, glutamic oxalacetic transaminase and r-glutamyltranspeptidase abnormity which are caused on a hepatitis virus carrier and achieves high negative conversion ratio of the viruses without the phenomena of rebound, drug resistance and variation after being stopped from being taken.
Owner:CHENGDU JIANJIANG PHARMA FACTORY
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