The invention discloses a constructing device and a constructing method of an
artificial liver lobule functional unit. The constructing device comprises a liver plate basal layer, a
liver parenchyma cell zone and a fluid passageway layer, wherein a liver plate patterning groove is formed in the liver plate basal layer through
processing; the fluid passageway layer comprises a fluid inlet passageway, an endothelial
cell oriented growth zone and a fluid outlet passageway. The constructing method corresponding to the constructing device comprises the following steps: paving collagen containing
liver parenchyma cells to the inner part of the liver plate patterning groove, bonding the liver plate basal layer with the fluid passageway layer after solidifying the collagen, pouring an endothelialcell suspension solution to the endothelial
cell oriented growth zone, and enabling a
cell culture medium solution to unidirectionally flow in the fluid inlet passageway, the endothelial cell orientedgrowth zone and the fluid outlet passageway after increasing endothelial cells to a certain amount. According to the constructing device and the constructing method, disclosed by the invention, reallayer-to-layer position relationship between
endodermis and
liver parenchyma cells is realized under dynamic culturing, the response of the liver
parenchyma cells to physicochemical and biochemical
irritation under the protection of the
endodermis of blood vessels more tends to reality, and a more accurate result can be obtained when the
artificial liver lobule functional unit is applied to
drug screening, case research and therapy detection.