Artificial liver organ and preparation method and application thereof

Abstract

The invention relates to the field of biological materials and biomedical engineering, and particularly relates to an artificial liver organ and a preparation method and application thereof. The artificial liver organ comprises an endothelial cell layer, a hepatic stellate cell layer and a hepatic parenchyma cell layer; the different cell layers are arranged in a bionic and close mode, and the distance between different kinds of adjacent cells is smaller than 30 micrometers; the mechanical property of the artificial liver organ is 500Pa-1MPa; and the characteristic length of the artificial liver organ is 50-1000 micrometers. The constructed liver organ comprises liver cells, endothelial cells and hepatic stellate cells, the structure and physiological function of the liver Disse gap can be simulated, and the requirements of in-vitro liver physiological / pathological research, drug testing and drug development, tissue engineering and regenerative medicine are met. The artificial liver organ can be induced into various liver disease models at the same time, and corresponding pathological models can be constructed according to requirements.

Description

technical field [0001] The invention relates to the fields of biomaterials and biomedical engineering, in particular to an artificial liver organoid and its preparation method and application. Background technique [0002] The liver is the largest internal organ in the human body, and it undertakes physiological functions such as metabolism of toxic substances, metabolism and synthesis of lipids and carbohydrates. The space of disse in the liver is the physiological structure of the liver, which is composed of hepatic parenchymal cells, hepatic sinusoidal endothelial cells, hepatic stellate cells and extracellular matrix components. Hepatic parenchymal cells undertake multiple functions such as biosynthesis, lipid metabolism and detoxification. The microvessel structure formed by hepatic sinusoidal endothelial cells is the site for material exchange between hepatocytes and blood, and blood flow stimulation in microvessels plays an important role in the process of liver stru...

AI Technical Summary

Problems solved by technology

At present, there are many reports on the construction of liver organoids based on liver stem cells, mesenchymal stem cells, endothelial cells, hepatic cell lines, hepatic stellate cell lines, embryonic stem cells, and induced pluripotent stem cells, but liver Disse gap organoids are still scarce.

In addition, at present, the method of directly embedding large pieces of hydrogel is mostly used in the construction of organoids. This method affects the supply of nutrients and oxygen, resulting in low production efficiency and poor uniformity of organoids.

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