15 results about "Endoderm cell" patented technology

Described are small molecule cocktails that enable de novo derivation and long-term culture of bovine extraembryonic endoderm cells (bXENs). Methods of using the small molecule cocktails to generate bXENs are also described. Methods of using the bXENs to form blastocyst models or to maintain the stemness of bovine ESCs and prevent them from differentiation are also described.

The invention relates to the technical field of biology, in particular to a method for efficiently obtaining human definitive endoderm cells in vitro, which comprises the following steps: S1, inoculating human pluripotent stem cells into matrigel, and performing maintenance culture for 16-24 hours by using a stem cell maintenance culture medium; s2, from Day-2 to Day-1, absorbing and abandoning the stem cell maintenance culture medium, adding a pretreatment culture medium for culture, and changing the pretreatment culture medium once every day; the pretreatment culture medium is a stem cell maintenance culture medium added with dimethyl sulfoxide; s3, carrying out Day0 to Day2, absorbing and abandoning the pretreatment culture medium, adding a definitive endoderm differential culture medium, carrying out induced culture, and changing the definitive endoderm differential culture medium once a day; the definitive endoderm differential culture medium is an RPMI1640 culture medium added with fetal calf serum and activin A; s4, Day3, obtaining the required human definitive endoderm cells, and solving the problems that the existing definitive endoderm differentiation efficiency and cell population purity are limited and are influenced by batch-to-batch variation.

The present application relates to the technical field of biology, and particularly discloses a method for culturing and obtaining foregut endoderm cell spheroids and application thereof. The method for culturing and obtaining foregut endoderm cell spheroids of the present application obtains foregut endoderm cell spheroids by inducing differentiation of stem cells on a culture surface with a PDA-matrigel coating. The culture method of the present application can ensure the differentiation efficiency of DE and AFE adherent cells and spheroids, promote the generation of AFE cell spheroids and the separation from the adherent cell layer, reduce the edge rolling of AFE adherent cells, prolong the production time of AFE cell spheroids, significantly improve the yield of AFE cell spheroids, and provide a basis for subsequent differentiation and culture of lung organoids.

While studies have been made on a method of selectively inducing differentiation from pluripotent stem cells into enterocyte-like cells, provided is an excellent and high-functionality enterocyte-like cell population that can be stably tested for multi-drug metabolism and permeability, and also provided an efficient method of producing cells. The method of inducing differentiation from pluripotent stem cells into enterocyte-like cells includes the following steps (1) and (2): (1) a step of inducing differentiation from pluripotent stem cells into definitive endoderm cells; and (2) a step of culturing the definitive endoderm cells in a system containing CHIR99021, followed by induction of differentiation into intestinal progenitor cells. A cell population of enterocyte-like cells is more effectively obtained by seeding and culturing enterocyte-like cells produced by the method of inducing differentiation, in a base material for organoid production or a base material for two-dimensional culture.

The present disclosure provides cell-based compositions for treating diabetes, methods for identifying cells that preferentially differentiate into endoderm cells, and methods for preparing insulin-producing pancreatic cells, as well as related methods of use for treating diseases related to insulin deficiency.

Embodiments of the present disclosure relate to novel compositions and methods for generating thymocytes. In some embodiments, thymocytes can be differentiated from pluripotent stem cells (PSCs), anterior primitive streak (APS) cells, definitive endoderm (DE) cells, anterior foregut endoderm (AFE) cells, pharyngeal endoderm (PE) cells, ventral pharyngeal endoderm (VPE) cells, and third pharyngeal pouch endoderm (TPPE) cells using the compositions and methods disclosed herein. In certain embodiments, thymocytes generated by the compositions, systems, and methods disclosed herein can be used to treat health conditions.

The present invention relates to methods of efficiently generating pancreatic endoderm from human pluripotent stem (PS) cell derived human definitive endoderm. The present invention also relates to pancreatic endoderm cells obtained by the methods of the invention. Finally, the present invention relates to culture medium and composition comprising a RAR antagonist and uses of said RAR antagonist in the induction of pancreatic endoderm cells. The present invention provides a more homogenous and synchronised pancreatic cell population, with increased efficiency.

The invention discloses a method for preparing intestinal organs from human induced pluripotent stem cells, and belongs to the technical field of biomedicine. A related culture differentiation system is optimized, stem cells are efficiently induced to be sequentially differentiated into endoderm cells and midgut and posterior gut cells, and finally intestinal organs with a complete intestinal tissue structure are formed. The method comprises the following key steps: amplifying stem cells by using a special culture medium, taking 3-5 generations of cells, and carrying out synergistic induction on the cells by Activin A and Wnt3a to differentiate into an endoderm; fGF-4, CHIR99021 and Y-27632 are used for promoting the formation of middle and posterior intestine spheres and the maturation of precursor cells; and finally, forming the organoid in a Matrigel three-dimensional scaffold and a compound factor culture medium of EGF (Epidermal Growth Factor), Noggin, RSPO1, N2 and B27. The method can be used for intestinal development mechanism research, disease model construction, drug screening and regenerative medicine, and particularly can be used for establishing a model by utilizing the specific stem cells of the patient in personalized medicine, so that precise drug testing and treatment scheme optimization are realized.

The present invention relates to a method for accelerated and cost-effective generation of insulin-producing beta cells from pluripotent stem cells using a fully defined, small molecule-based differentiation protocol. Further, the present invention relates to a method for obtaining pancreatic endoderm cells from posterior foregut cells. The use of the cells obtained with the method according to the invention is described as well.

The present invention provides: a method for producing pancreatic endoderm cells, the method comprising a step of culturing pancreatic endoderm cells in a culture medium containing a ROCK inhibitor, and KGF and / or EGF; and pancreatic endoderm cells produced by said method.

The invention relates to the field of biomedical engineering and disease model construction, in particular to a construction method and application of a full-spectrum liver organoid. The construction method comprises the following steps: respectively differentiating induced pluripotent stem cells from human umbilical cord to obtain transitional hepatic endoderm cells, endothelial progenitor cells, red myeloid progenitor cells, hepatic stellate cells and diaphragm mesenchymal stem cells; transition hepatic endoderm cells, endothelial progenitor cells, red myeloid progenitor cells, hepatic stellate cells and diaphragm mesenchymal stem cells are inoculated into a 3D culture system according to the cell number ratio of (9-11): (6-8): (1-2): (1-2): (1-2); and performing self-assembly co-culture on the seed cells in a 3D culture system until the liver function is mature, thereby obtaining the full-spectrum liver organoid. The technical problem that physiological authenticity, functional integrity and application universality of an existing liver model need to be improved is solved.

The invention relates to the technical field of biology, and particularly discloses a method for inducing front anterior intestine endoderm cells to differentiate to form lung progenitor cells and application of the method. The method for inducing anterior front intestine endoderm cells to differentiate to form the lung progenitor cells comprises the step of inducing anterior front intestine endoderm single cells into the lung progenitor cells by using a lung progenitor cell culture medium in a solidified 3D culture composite adhesive, the 3D culture composite adhesive is prepared from 3D culture hydrogel and polydopamine nanoparticles modified by polyethylene glycol. The cell three-dimensional culture system constructed by mixing the PEG-PDA NPs and the 3D culture hydrogel can promote differentiation of the human pluripotent stem cells to the lung progenitor cells, the differentiation efficiency and quality are improved, and a good foundation is laid for subsequent efficient formation of mature lung organs with trachea and alveolar characteristics.

This disclosure provides cell-based compositions for the treatment of diabetes, methods for identifying cells that preferentially differentiate into endoderm cells, and methods for preparing insulin-producing pancreatic cells, as well as related methods of use for the treatment of insulin deficiency-related diseases.

The invention discloses a universal type definitive endoderm culture medium for inducing pluripotent stem cells to differentiate into definitive endoderm cells and a culture method, and relates to the technical field of stem cell biology and regenerative medicine. The universal typing endoderm culture medium comprises a basic culture medium, polyvinyl alcohol, an animal-origin-free B-27 supplement, a TGF-beta activator, a Wnt signaling pathway activator, a JNK-JUN inhibitor, a PI3K / mTOR inhibitor, L-ascorbic acid and a ROCK inhibitor, wherein the basic culture medium is used for providing nutritional ingredients required by cell growth; the universal typified endoderm culture medium comprises a typified endoderm culture medium 1 and a typified endoderm culture medium 2, according to the culture method, the general type typing endoderm culture medium is constructed to obtain the typing endoderm cell balls, and the general type typing endoderm culture medium is definite in component and does not contain animal-derived components, so that the general type typing endoderm culture medium meets the requirement of a drug supervision institution on chemical component limitation of cell treatment products; high-density culture is carried out on the typified endoderm cells through suspension culture, so that the method is more suitable for production requirements.

Among the various aspects of the present disclosure is the provision of methods for the generation of stem cell-derived beta cells (SC-β), stem cell-derived islets (SC-islets), definitive endoderm cell, pancreatic progenitor cells, and uses thereof. The various cells are generated by contacting human pluripotent stem cells (hPSCs) with an amount of a cytoskeleton depolymerizing agent during initial stem cell differentiation into definitive endoderm cells. A method of treatment of a subject diagnosed with a diabetes condition by transplanting administering a therapeutically effective amount of the stem cell-derived islets (SC-islets) into the subject is also disclosed.