The invention relates to a method for inducing differentiation into NK cells from human embryonic stem cells, belonging to the technical field of genetic engineering; the method includes the recovery and passage of human embryonic stem cells, inducing mesodermal cells, and inducing CD34 cells + Hematopoietic stem cells, isolation and purification of CD34 + Hematopoietic stem cells, induced to obtain NK cells, expanded and cultured; the human embryonic stem cells are human embryonic stem cell line H9; the recovery and passage of the human embryonic stem cells, the human embryonic stem cells are revived and passaged, passed to the second generation, and the cells are confluent When the ratio is more than 80%, it is used to induce mesodermal cells; the present invention uses human embryonic stem cells to induce differentiation to obtain NK cells, and when the effect-target ratio is 20:1, the killing rate of HELA tumor cell line The killing rate of the LOVO tumor cell line was 91.24%.