10 results about "Cell differentation" patented technology

The current disclosure provides methods and compositions that provide for the insertion of transgenes in stem or progenitor cells without the deleterious effects to T cell differentiation by in vitro T cell differentiation methods. To accomplish this, the inventors have discovered that expression of the transgenes under the control of promoter regions, such as CD8a or CD8b, allows for the coordinated expression pattern that provides for both: 1) high expression of the transgene in mature T cells and 2) a coordinated level of expression of the transgene throughout the in vitro differentiation method that allows for the production of a population of mature T cells.

The invention relates to the technical field of cell differentiation and the technical field of myocardial tissue regeneration medicine, in particular to an induction culture medium and a method for rapidly obtaining myocardial balls capable of jumping spontaneously, hiPSC-sourced myocardial cells are obtained through co-culture with hMSC, and a differentiation culture medium is combined, so that myocardial cell clones harvested by D7 are more independent, and the differentiation efficiency is improved. The myocardial cells are naturally separated from other non-myocardial cells and can be rapidly cloned, screened and purified by using physical and enzyme-free reagents, so that the step of screening and purifying a culture medium in a traditional method is omitted, and the time and differentiation cost are saved.

This invention provides a concentration gradient cell culture system that solves the problem of large errors in manual drug addition and the difficulty in constructing a stable, continuous concentration gradient in studies on the control of osteoclast progenitor cell differentiation using propionic acid-GPR43. [Solution] The system comprises a housing assembly with an inner container, a first liquid storage cylinder 2, a second liquid storage cylinder 3, a tuned crossbeam 4, an adjustable screw rod 5, a flow-dividing branch pipe array frame, a spiral mixing unit, a porous diffusion plate, and a culture tray 9. The tuned crossbeam is driven by the adjustable screw rod to synchronously supply liquid from both liquid storage cylinders. After gradient distribution by the flow-dividing branch pipe array frame and mixing by the spiral mixing unit, the porous diffusion plate forms a continuous and stable propionic acid concentration gradient field on the culture tray. This avoids manual errors, provides accurate concentration gradients to RAW264.7 and BMMs cells in a single experiment, and supports the calculation of dose-effect curves and IC50, as well as the elucidation of the mechanism of osteoclast differentiation control by propionic acid-GPR43 signaling.

This invention discloses a specific expression regulatory element for the hornless target gene RXFP2 in goats and its application. The sequence of the regulatory element is SEQ ID No. 1. Through single-cell gene expression and chromatin accessibility analysis of fetal bovine horn bud tissue, this invention discovered a specific, open regulatory element (chr12:29279059-29279559) upstream of RXFP2 in horn bud tissue. This element possesses a binding motif for RUNX2, a core transcription factor promoting osteoprogenitor cell differentiation, and is highly conserved in horned animals. Its location in the goat genome is chr12:57440948-57441449. Dual-luciferase assays verified that this regulatory element mediates the interaction between transcription factors RUNX2 and RXFP2, achieving specific high expression of RXFP2 in horn bud tissue. By knocking out the regulatory element region (chr12:57440800-57441678) in goat embryos using CRISPR-Cas9, goat embryos with gene-edited knockout of the hornless target gene RXFP2 specifically expressing regulatory elements were successfully prepared. This invention further elucidates the origin, evolution, and skin ossification mechanisms of horn organs, providing new targets for hornless livestock breeding.

The technology relates in part to methods for detecting the activity of IL-40 and modified versions thereof. The technology also relates in part to uses of IL-40 for promoting cell differentiation.

The utility model relates to the technical field of calculus bovis cultivation, and discloses a bioreactor for in-vitro cultivation of calculus bovis, which comprises a reactor body, a material uniformizing mechanism arranged in an inner cavity of the reactor body, a plurality of groups of oxygen supply tanks arranged at the upper end of the reactor body, and a first electromagnetic valve arranged at the lower end of each group of oxygen supply tank, an oxygen supply mechanism is arranged at the bottom of the inner cavity of the reactor body and is arranged below the refining mechanism; through cooperation of the oxygen supply mechanism, the reactor body and the material uniformizing mechanism, oxygen can be uniformly introduced into the bottom of the inner cavity of the reactor body through the oxygen supply mechanism, so that the contact range between the oxygen and cells is expanded, and meanwhile, under the action of the material uniformizing mechanism, the oxygen can flow in the reactor body along with bile, so that the bile is uniformly dispersed. Therefore, the oxygen can be uniformly supplied to the cells, and the mixing uniformity of the bile and various raw materials for promoting cell differentiation is improved, so that the cultivation efficiency of the calculus bovis is improved.

The invention discloses a system for promoting in-vitro differentiation of chicken oogonial stem cells and forming blastocysts, and belongs to the technical field of stem cell differentiation culture. According to the invention, retinoic acid (RA) and vitamin C (VC) are added on the basis of an oogonial stem cell culture medium to induce cells to start meiosis, and then follicle stimulating hormone (FSH), human chorionic gonadotropin (HCG), glial cell-derived neurotrophic factor (GDNF), progesterone (P4) and rosiglitazone are further added on the basis of the culture medium containing RA and VC to promote the recovery of meiosis, so that the effect of promoting meiosis is achieved. A complete system that the chicken oogonial stem cells are differentiated in vitro and form blastocysts is creatively established by inducing formation of secondary oocytes, direct evidence is provided for in-vitro reconstruction of poultry ovum generation, and a technical basis and application prospects are provided for preservation of female germ cells and large-scale production of the chicken oocytes.

The invention discloses application of fructo-oligosaccharide in improvement of growth performance and intestinal development of suckling piglets and a nutrition regulator of the fructo-oligosaccharide, the fructo-oligosaccharide is applied to the suckling piglets as an early nutrition intervention means, the intestinal villus height of the suckling piglets is remarkably increased, the crypt depth is reduced, and the growth performance of the suckling piglets is improved. The improvement of the activity of small intestine disacidase and the change of small intestine stem cell differentiation marker gene mRNA indicates that the fructo-oligosaccharide improves the growth performance of the suckling piglets by promoting the intestinal tissue morphology, digestion and absorption functions and small intestine stem cell differentiation capacity of the suckling piglets.

The invention discloses an adipogenic induced differentiation culture medium suitable for natural immortalized cells of chick embryos, an adipogenic differentiation method and application of the adipogenic induced differentiation culture medium and the adipogenic differentiation method, and belongs to the field of poultry cell differentiation. The chick embryo naturally immortalized cells are obtained by naturally immortalizing and screening 9-11-day-old SPF chick embryos, and auxiliary factors of the induced differentiation medium comprise dexamethasone, insulin, IBMX, rosiglitazone, oleic acid, glutamine, transferrin and BSA. The culture medium suitable for the natural immortalized cells of the chick embryos has a good adipogenic differentiation effect, almost all cells have lipid droplets after the natural immortalized cells of the chick embryos are subjected to in-vitro induced differentiation for 48 hours, the cell differentiation efficiency reaches about 95% or above, the differentiation efficiency of the natural immortalized cells of the chick embryos to an adipocyte lineage is remarkably improved, and the differentiation rate of the natural immortalized cells of the chick embryos to the adipocyte lineage is greatly improved. And the differentiation time is greatly shortened, so that the method has important significance on the research on the lipogenic differentiation mechanism of the fiber-derived cells and the preparation of cell culture meat.