64 results about "Cell differentation" patented technology

A method for treating cells and/or nuclear transfer units and/or stem cells in culture with such compounds, individually or in combinations, is described. The method results in a globally hypomethylated genome and a restoration of cell differentiation and/or developmental potential, or potentiality. In addition, a method for the in vitro production of reprogrammed cells which have had differentiation potential (totipotential, pluripotential, or multipotential) restored by demethylating the genome is described.

An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Also, methods for improving nuclear transfer efficiency by genetically altering donor cells to inhibit apoptosis, select for a specific cell cycle and/or enhance embryonic growth and development are provided.

The invention provides aging-resistant eye cream. The aging-resistant eye cream is prepared from 4-8 parts by mass of witch hazel extract, 5-10 parts by mass of pomegranate seed extract, 2-4 parts by mass of soybean extract, 2-4 parts by mass of Cyanotis arachnoidea CB.Clarke extract, 4-6 parts by mass of apple leaf extract, 4-8 parts by mass of aloe gel, 10-15 parts by mass of vitamin E, 2-4 parts by mass of alpha-arbutin, 4-8 parts by mass of hyaluronic acid and 10-20 parts by mass of glycerin. The aging-resistant eye cream is rich in pure natural plant extract, does not contain any harmful components and is mild and non-irritant. In use, the aging-resistant eye cream with the volume the same as that of soya bean is taken, the aging-resistant eye cream is smeared on skin around the eyes and the skin is massaged gently. The aging-resistant eye cream can improve skin elasticity by promoting cell differentiation and collagen synthesis, and can prevent and reduce eye wrinkle by promoting regeneration of skin around the eyes. The whitening component of the aging-resistant eye cream can effectively remove melanin around eyes, eliminate dark eye circles and make eyes bright.

The invention relates to a method for inducing the differentiation of mesenchymal stem cells of human embryo livers into islet beta-like cells and stably expressing insulin and special induced liquid thereof. The method comprises the following steps: separating, purifying and amplifying mesenchymal stem cells of human embryo livers; then taking out cells formed after the third generation and inducing the cells by the special induced liquid to obtain islet beta-like cell mass capable of expressing the insulin stably. The induced liquid can be obtained by adding TAT-PDX1 fusion protein in cell culture fluid. The method can realize in-vitro induction of mesenchymal stem cells of human embryo livers into islet beta-like cells capable of secreting the insulin stably. When transplanted in the body of a diabetic patient, the obtained islet beta-like cells can further adjust blood sugar level so as to realize cell therapy of diabetes. Therefore, research in the field has broad clinic application prospect and can bring greater economic benefit and social benefit.

The invention relates to a caspase-1-ES cell line induced by mifepristone. The collection number of the caspase-1-ES cell line is CCTCC No.C201193. The invention also provides a preparation method and application of the cell line. The invention has the advantages that: caspase-1 is transferred into an embryonic stem (ES) cell for the first time, and the differential potential of the cell is not influenced; the caspase-1 is highly expressed in the ES cell for the first time, it is proved that activated caspase-1 initiates DNA damage to cause death of undifferentiated caspase-1-ES cells in vitro, and a risk of nodule formation caused by ES cell transplantation is reduced; it is discovered that the highly expressed caspase-1 in the differentiated ES cell does not obviously influence the cell for the first time, and the treatment effect of the ES cell is maintained; and the safety of embryonic stem cell transplantation is improved, the effect of treating parkinson disease and other neurologic diseases is remained, and the caspase-1-ES cell line can be widely applied to stem cell transplantation.

An improved method of nuclear transfer involving the transplantation of donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation.

The invention provides a method for obtaining human Flooor Plate (FP) cells in vitro. The method comprises the following steps: a human embryonic stem cell line H9 is modified through a gene, and an NKX2.2-CDS region and an inducible Tet-on expression system are inserted into a human chromosome 19 to obtain a human embryonic stem cell line H9-iOE-NKX2.2, of which the NKX2.2 expression level accurately depends on exogenous DOX dosage; and on the basis of H9-iOE-NKX2.2, the H9-iOE-NKX2.2 is cultured for 12 days by using a specific nerve induction culture medium, and meanwhile, the expression of NKX2.2 is induced at a low level in the first 6 days. According to the invention, the functional human Floor Plate cells secreting SHH can be rapidly obtained, and the human Floor Plate cells obtained by differentiation can be applied to in-vitro research of human neural circuit constitution, treatment of various brain development disorder diseases caused by FP cell abnormality and expansion of in-vitro brain cell differentiation methods.

The invention provides a method for directionally differentiating induced multipotent stem cells into inner ear hair cell-like cells. A culture medium with definite chemical components is mainly utilized, small molecule compounds are added at proper time to induce and differentiate iPSC into inner ear progenitor cells, and then the inner ear progenitor cells and chick embryo elliptical capsule cells are co-cultured, so that the inner ear hair cell-like cells are obtained. According to the method, the induced multipotent stem cells can be effectively and directionally differentiated into the inner ear hair cell-like cells, and the inner ear hair cell-like cells obtained through differentiation have cilia bundle structures, express inner ear hair cell specific proteins and have hair cell electro-physiological functions.

The invention provides a marker for evaluating hepatic lineage cell maturity, a biomics kit and a construction method. According to the invention, a microRNA set and a gene set which have time sequence characteristics and have a regulatory relationship in cell development are elaborated from a new perspective for the first time, a transcription factor-microRNA-target gene regulatory network related to hepatocyte differentiation and maturation regulation is established, and a gene tag and a microRNA tag of hepatolineage specificity are obtained, wherein these tags or combinations thereof can be used as potential new markers for hepatic lineage cells. The invention also provides a biomics kit for the hepatic lineage cells, and a new scheme is provided for large-scale amplification of stable hepatic lineage cells and monitoring of in-vitro culture states of the hepatic lineage cells.

The invention provides a culture method for producing adventitious buds through one-step induction of Blumea balsamifera root cell differentiation, which comprises the following steps: selecting a root segment of Blumea balsamifera as an explant for culture, designing an adventitious bud induction culture medium, and inducing the explant to directly differentiate the adventitious buds only by using the culture medium; according to the method, the blank of using the blumea balsamifera root as the explant is filled, and the effective explant source of the blumea balsamifera in the propagation process is increased. Besides, the root cells of the blumea balsamifera are directly differentiated to form the adventitious buds, and the process of forming calluses is not needed, so that the technical links are simplified, the variation of regenerated buds and the generation of chimeras are reduced, the accuracy of directional mutagenesis or genetic transformation of the blumea balsamifera is improved, and the method can be applied to breeding of the blumea balsamifera and can be applied to breeding of the blumea balsamifera. And time and labor cost can be remarkably saved, and the working efficiency is improved.