Method for inducing the differentiation of mesenchymal stem cells of human embryo livers into islet beta-like cells and stably expressing insulin and special induced liquid thereof

A technology of mesenchymal stem cells and cell differentiation, applied in the field of induced stem cell differentiation, can solve safety problems and other problems, and achieve the effect of avoiding biological safety problems
CN101580818AInactive Publication Date: 2009-11-18JINAN UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JINAN UNIVERSITY
Publication Date
2009-11-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for inducing the differentiation of mesenchymal stem cells of human embryo livers into islet beta-like cells and stably expressing insulin and special induced liquid thereof. The method comprises the following steps: separating, purifying and amplifying mesenchymal stem cells of human embryo livers; then taking out cells formed after the third generation and inducing the cells by the special induced liquid to obtain islet beta-like cell mass capable of expressing the insulin stably. The induced liquid can be obtained by adding TAT-PDX1 fusion protein in cell culture fluid. The method can realize in-vitro induction of mesenchymal stem cells of human embryo livers into islet beta-like cells capable of secreting the insulin stably. When transplanted in the body of a diabetic patient, the obtained islet beta-like cells can further adjust blood sugar level so as to realize cell therapy of diabetes. Therefore, research in the field has broad clinic application prospect and can bring greater economic benefit and social benefit.
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Description

technical field

[0001] The invention relates to the technical field of inducing stem cell differentiation, in particular to a method for inducing human fetal liver mesenchymal stem cells to differentiate into islet β-like cells and stably expressing insulin and a special inducing solution thereof. Background technique

[0002] Diabetes has become the main killer threatening human life and health in the 21st century. At present, taking drugs and injecting insulin is a commonly used method in various treatment measures for the etiology and pathology of diabetes, but taking drugs and injecting insulin will not only bring a heavy economic burden, but also cause serious complications; in recent years, transplantation Pancreatic islets are initially effective in treating diabetes, but they face two major problems: insufficient tissue sources and immune rejection. Gene / cell therapy has attracted more and more attention. Pancreatic-duodenal homeobox 1 gene (PDX1) is a major regula...

Claims

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